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Ectopic overexpression of a salt stress-induced pathogenesis-related class 10 protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco

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Abstract

Pathogenesis-related proteins are induced in plants in response to stress, pathogen attack or abiotic stimuli, thus playing a cardinal role in plant defense system. A cDNA containing the full-length ORF, AhSIPR10 (474 bp, GenBank acc. no. DQ813661), encoding a novel Salinity-Induced PR class 10 protein was isolated from callus cell lines of peanut (Arachis hypogaea). Real-time quantitative reverse transcription PCR (qRT–PCR) data showed rapid upregulation of AhSIPR10 transcription in peanut callus cultures across salinity, heavy metal, cold and mannitol-induced drought stress environments. Likewise, AhSIPR10 expression was also responsive towards defense/stress signaling molecules salicylic acid (SA), methyl jasmonate, abscisic acid (ABA) and H2O2 treatments. Methyl jasmonate or ABA-induced AhSIPR10 expression was, however, antagonized by SA treatment. A functional role of AhSIPR10 in alleviation of abiotic stress tolerance was further validated through its over-expression in tobacco. Analysis of T1 transgenic tobacco plants overexpressing AhSIPR10 gene showed enhanced tolerance to salt, heavy metal and drought stress through leaf disc senescence, chlorophyll content, seed set and germination assays, thus corroborating a role of salt inducible-PR10 protein in mitigation of abiotic stress-induced damage. Transgenic tobacco lines overexpressing AhSIPR10 displayed adequate photosynthetic CO2 assimilation rates under salt, heavy metal and drought stress environments.

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Abbreviations

ABA:

Abscisic acid

AhSIPR10 :

Arachis hypogaea salinity-induced PR class 10

JA:

Jasmonic acid

MeJA:

Methyl jasmonate

MS:

Murashige and Skoog medium

PR (10):

Pathogenesis-related (class 10)

SA:

Salicylic acid

WT:

Wild-type

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Acknowledgments

Research fellowship from Council of Scientific and Industrial Research, India to Shalu Jain is gratefully acknowledged. This research work was supported by Department of Biotechnology, India (Grant no BT/PR10231/AGR/02/555/2007).

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  1. Shalu Jain

    Present address: Department of Plant Sciences, North Dakota State University, Fargo, ND, 58102, USA

  2. Mukesh Jain

    Present address: Agronomy Department, University of Florida, Gainesville, FL, 32610, USA

Authors and Affiliations

  1. Plant Developmental Biology and Transformation Lab, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Shalu Jain, Deepak Kumar, Mukesh Jain, Prerna Chaudhary & Neera Bhalla Sarin

  2. Department of Botany, University of Delhi, Delhi, 110007, India

    Renu Deswal

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  1. Shalu Jain
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Correspondence to Neera Bhalla Sarin.

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Jain, S., Kumar, D., Jain, M. et al. Ectopic overexpression of a salt stress-induced pathogenesis-related class 10 protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco. Plant Cell Tiss Organ Cult 109, 19–31 (2012). https://doi.org/10.1007/s11240-011-0069-6

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