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Large-scale somatic embryogenesis and regeneration of Panax notoginseng

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Abstract

An efficient system for inducing somatic embryogenesis in Panax notoginseng was established using shaker flasks and bioreactor cultures; furthermore, regenerated plantlets were successfully transferred to ex vitro soil conditions. Embryogenic callus was induced from segments of adventitious roots incubated on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 5 weeks of culturing. The highest frequency (100%) of somatic embryogenesis, with a mean of 32.7 somatic embryos per callus, was obtained on embryogenic callus incubated on a medium containing 0.5 mg/L 2,4-D. To scale-up somatic embryo formation, 10 g (~1.65 × 104) of early globular-stage somatic embryos were incubated in a 3 L airlift bioreactor containing 1.5 L 1/2 MS medium without plant growth regulators (PGRs) for a period of 4 weeks; these globular-stage somatic embryos then developed into cotyledonary embryos. When maintained on PGR-free medium, the cotyledonary embryos developed roots but did not develop shoots. However, when they were treated with gibberellic acid (GA3), they continued to germinate and transformed into plantlets after 2 weeks of culture. Plantlets with well-developed shoots and roots were transferred to an autoclaved vermiculite and perlite mixture, acclimatized for a period of 3 months and successfully transferred to forest mountain soil. Following overwintering, these plants produced new growth.

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Acknowledgments

This work was supported by the program of the DaQing Technology Office, and from the WCU project (R33-10157) of the Ministry of Education, Science & Technology (MEST), Republic of Korea.

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Authors and Affiliations

  1. College of Life Sciences, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China

    Xiang Ling You, Xiao Tan, Jin Ling Dai & Yu Hua Li

  2. Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chunchon, 200-701, Republic of Korea

    Yong Eui Choi

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  1. Xiang Ling You
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  2. Xiao Tan
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  3. Jin Ling Dai
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  4. Yu Hua Li
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Correspondence to Yong Eui Choi.

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You, X.L., Tan, X., Dai, J.L. et al. Large-scale somatic embryogenesis and regeneration of Panax notoginseng . Plant Cell Tiss Organ Cult 108, 333–338 (2012). https://doi.org/10.1007/s11240-011-0030-8

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