Abstract
Research has revealed that most chlorophyllous explants/plants in vitro have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Moreover, numerous studies have been conducted on improving the in vitro environment and investigating its effects on growth and development of cultures/plantlets on nearly 50 species since the concept of photoautotrophic micropropagation was developed more than two decades ago. These studies indicate that the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. This paper reviews the development and characteristics of photoautotrophic micropropagation systems and the effects of environmental conditions on the growth and development of the plantlets. The commercial applications and the perspective of photoautotrophic micropropagation systems are discussed.
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References
Afreen F, Zobayed SMA, Kozai T (2001) Mass-propagation of coffee from photoautotrophic somatic embryos. In: Morohoshi N, Komamine A (eds) Molecular breeding of woody plants. Elsevier Science B.V, The Netherlands, pp 355–364
Afreen-Zobayed F, Zobayed SMA, Kubota C, Kozai T (1999) Supporting material affects the growth and development of in vitro sweet potato plantlets cultured photoautotrophically. In Vitro Cell Dev Plant 35:470–474
Aitken-Christie J, Kozai T, Smith MAL (eds) (1995) Automation and environmental control in plant tissue culture. Kluwer Academic Publishers, Dordrecht, p 574
Arigita L, González A, Sánchez Tamés R (2002) Influence of CO2 and sucrose on photosynthesis and transpiration of Actidinia deliciosa explants cultured in vitro. Physiol Plant 115:166–173
Couceiro MA, Afreen F, Zobayed SMA, Kozai T (2006) Enhanced growth and quality of St. John’s wort (Hypericum perforatum L.) under photoautotrophic in vitro conditions. In Vitro Cell Dev Biol Plant 42:278–282
Cui YY, Hahn EJ, Kozai T, Paek KY (2000) Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro. Plant Cell Tiss Organ Cult 62:219–226
DaSilva T, Giang DDT, Tanaka M (2006) Photoautotrophic micropropagation of Spathiphyllum. Photosynthetica 44:53–61
Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. Sci Hort 14:335–345
Ermayanti TM, Imelda M, Tajuddin T, Kubota C, Kozai T (1999) Growth promotion by controlling the in vitro environment in the micropropagation of tropical plant species. In: Proceedings of international workshop on conservation and sustainable use of tropical bioresources, Nov 9–10, Tokyo, Japan, pp 10–25
Fujiwara K, Kozai T (1995) Physical microenvironment and its effects. In: Aitken-Christie J, Kozai T, Smith MAL (eds) Automation and environmental control in plant tissue culture. Kluwer Academic Publishers, Dordrecht, pp 319–369
Fujiwara K, Kozai T, Watanabe I (1987) Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets. J Agr Met 43:21–30
Fujiwara K, Kozai T, Watanabe I (1988) Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages. Acta Hort 230:153–158
Heo J, Kozai T (1999) Forced ventilation micropropagation system for enhancing photosynthesis, growth and development of sweet potato plantlets. Environ Control Biol 37:83–92
Heo J, Wilson SB, Kozai T (2001) A forced ventilation micropropagation system for production of photoautotrophic sweetpotato plug plantlets in a scaled-up culture vessel, I. Growth and uniformity. Hort Technol 11:90–94
Houllou-Kido LM, Silva KS, Rivas R, Dias ALF, Alves GD (2009) Viability of Noppalea cochenilifera (cv. IPA Sertania) photoautotrophic micropropagation. Acta Hort 811:309–313
Jeong BR, Fujiwara K, Kozai T (1995) Environmental control and photoautotrophic micropropagation. Hort Rev 17:125–172
Kirdmanee C, Kitaya Y, Kozai T (1995a) Effects of CO2 enrichment and supporting material on photoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro. In Vitro Cell Dev Biol Plant 31:144–149
Kirdmanee C, Kitaya Y, Kozai T (1995b) Effects of CO2 enrichment and supporting material on growth, photosynthesis and water potential of Eucalyptus shoots/plantlets cultured photoautotrophically in vitro. Environ Control Biol 33:133–141
Kirdmanee C, Kitaya Y, Kozai T (1995c) Rapid acclimatization of eucalyptus plantlets by controlling photosynthetic photon flux density and relative humidity. Environ Control Biol 33:123–132
Kitaya Y, Ohmura Y, Kubota C, Kozai T (2005) Manipulation of the culture environment on in vitro air movement and its impact on plantlets photosynthesis. Plant Cell Tiss Organ Cult 83:251–257
Kool LT, Keng CL, Toe CTK (1999) In vitro rooting of Sentang shoots (Azadirachta excelsa L.) and acclimatization of the plantlets. In vitro Cell Dev Biol Plant 35:396–400
Kozai T (1991) Photoautotrophic micropropagation. In Vitro Cell Dev Biol 27:47–51
Kozai T (1998) Transplant production under artificial light in closed systems. In: Lu HY, Sung JM, Kao CH (eds.) Proceedings of 3rd Asian crop science conference, Taichung, Taiwan, pp 296–308
Kozai T (2007) Propagation, grafting and transplant production in closed systems with artificial lighting for commercialization in Japan. Propagat Ornament Plants 7:145–149
Kozai T, Iwanami Y (1988) Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of Carnation (Dianthus caryophyllus L.,) in tissue culture during the preparation stage. J Jpn Soc Hortic Sci 57:279–288
Kozai T, Kubota C (2001) Development a photoautotrophic micropropagation system for woody plants. J Plant Res 114:525–537
Kozai T, Kubota C (2005) Concepts, definitions, ventilation methods, advantages and disadvantages. In: Kozai T, Afreen F, Zobayed SMA (eds) Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system. Springer, Dordrecht, pp 19–30
Kozai T, Sekimoto K (1988) Effects of number of air exchanges per hour of the closed vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and growth of strawberry plantlets in vitro (in Japanese). Environ Control Biol 26:21–29
Kozai T, Xiao Y (2005) A commercialized photoautotrophic micropropagation system. In: Gupta S, Ibaraki Y (eds) Plant tissue culture engineering. Berlin, Springer, pp 355–371
Kozai T, Koyama Y, Watanabe I (1988) Multiplication and rooting of potato plantlets in vitro with sugar medium under high photosynthetic photon flux. Acta Hort 230:121–127
Kozai T, Kubota C, Heo J, Chun C, Ohyama K, Niu G, Mikami H (1998) Towards efficient vegetative propagation and transplant production of sweetpotato (Ipomoea batatas (L.) Lam.) under artificial light in closed systems. In: Proceedings of international workshop on sweetpotato production system toward 21st Century, Miyazaki, Japan, pp 201–214
Kozai T, Chun C, Afreen F, Ohyama K (2000) Necessity and concept of the closed transplant production system. In: Kubota C, Chun C (eds) Transplant production in the 21st century. Kluwer Academic publishers, Dordrecht, pp 3–19
Kozai T, Afreen F, Zobayed SMA (2005) Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system. Springer, Dordrecht, p 315
Kubota C, Chun C (eds) (2000) Transplant production in the 21st century. Kluwer Academic Publishers, Dordrecht
Kubota C, Kozai T (1992) Growth and Net photosynthetic rate of solanum tuberosum in vitro under forced ventilation. Hort Sci 27:312–1314
Kubota C, Afreen F, Zobayed SMA (2005) Plant species successfully micropropagated photoautotrophically. In: Kozai T, Afreen F, Zobayed SMA (eds) Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system. Springer, Dordrecht, pp 243–266
Kurata K, Kozai T (1992) Transplant production systems. Kluwer Academic Publishers, Dordrecht
Liao F, Wang B, Zhang M, Xu F, Lian F (2007) Response to sucrose-free culture and diffusive ventilation of plantlets in vitro of Gerbera jamesonii and photoautotrophic growth potential. Acta Hort 764:257–264
Liu W, Yang Q (2008) Integration of mycorrhization and photoautotrophic micropropagation in vitro: feasibility analysis for mass production of mycorrhizal transplants and inoculants of arbuscular mycorrhizal fungi. Plant Cell Tiss Organ Cult 95:131–139
Lovato P, Guillemin JP, Gianinazzi S (1996) Application of commercial abuscular endomycorrhizal fungal inoculants to the establishment of micropropagated grapevine root-stock and pineapple plants. Agronomie 12:673–880
Lucchesini M, Mensuali-Sodi A, Massai R, Gucci R (2001) Development of autotrophy and tolerance to acclimatization of Myrtus communis transplants cultured in vitro under different aeration. Biol Plant 44:167–174
Lucchesini M, Monteforti G, Mensuali-Sodi A, Serra G (2006) Leaf ultrastructure, photosynthetic rate and growth of myrtle plantlets under different in vitro culture conditions. Plant Biol 50:161–168
Majada JP, Fall MA, Tadeo F, Sànchez-Tamés R (2002) Effects of natural ventilation on leaf ultrastructure of Dianthus caryophyllus L. cultured in vitro. In Vitro Cell Dev Biol Plant 38:272–278
Mosaleeyanon K, Zobayed SMA, Afreen F, Kozai T (2005) Relationships between net photosynthetic rate and secondary metabolite contents in St. Jon’s wort. Plant Sci 169:523–537
Nagae S, Takamura T, Watanabe T, Murakami A, Murakami K, Tanaka M (1996) In vitro shoot development of Eucalyptus citriodora on rockwool in the film culture vessel under CO2 enrichment. J For Res 1:227–230
Nguyen QT, Kozai T (2001) Growth of In Vitro Banana (Musa SPP.) shoots under photomixotrophic and photoautotrophic conditions. In Vitro Cell Dev Biol Plant 37:824–829
Nguyen TQ, Kozai T, Nguyen KL, Nguyen UV (1999a) Effects of sucrose concentration, supporting material and number of air exchanges of the vessel on the growth of in vitro coffee plantlets. Plant Cell Tiss Organ Cult 58:51–57
Nguyen TQ, Kozai T, Niu G, Nguyen UV (1999b) Photosynthetic characteristics of coffee (Coffea arabusta) plantlets in vitro in response to different CO2 concentrations and light intensities. Plant Cell Tiss Organ Cult 55:133–139
Nguyen QT, Kozai T, Heo J, Thai DX (2001) Photoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetic photon fluxes under carbon dioxide enriched conditions. Plant Cell Tiss Organ Cult 66:217–225
Nhut DT, Hong ITA, Watanabe H, Goi M, Tanaka M (2002) In vitro growth of banana plantlets cultured under red and blue light-emitting diode (LED) irradiation source. Acta Hort 575:117–123
Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under super bright red and blue light-emitting diodes (LEDs). Plant Cell Tiss Organ Cult 73:43–52
Nishimura M, Kozai T, Kubota C, Chun C (2001) Analysis of electric energy consumption and its cost for a closed-type transplant production system. SHITA J 13:204–209
Niu G, Kozai T (1997) Simulation of the growth of potato plantlets cultured photoautotrophically in vitro. Trans ASAE 40:255–260
Niu G, Kozai T, Kitaya Y (1996) Simulation of the time courses of CO2 concentration in the culture vessel and net photosynthetic rate of Cymbidium plantlets. Trans ASAE 39:1567–1573
Niu G, Kozai T, Hayashi M, Tateno M (1997) Simulation of the time courses of CO2 concentration in the culture vessel and net photosynthetic rate of potato plantlets cultured photoautotrophically and photomixotrophically in vitro under different lighting cycles. Trans ASAE 40:1711–1718
Ohyama K, Kozai T (1998) Estimating electric energy consumption and its cost in a transplant production factory with artificial lighting: a case study. J High Technol Agri 10:96–107
Pospisilova J, Solarova J, Catsky J, Ondrej M, Opatrny Z (1988) The photosynthetic characteristics during the micropropagation of tobacco and potato plants. Photosynthetica 22:205–213
Roberts AV, Smith EF (1990) The preparation in vitro of chrysanthemum for transplantation to soil. I. Protection of roots by cellulose plugs. Plant Cell Tiss Organ Cult 21:129–132
Serret MD, Trillas MI, Matas J, Araus JL (1996) Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation. Plant Cell Tiss Organ Cult 45:1–16
Tanaka M, Nagae S, Fukai S, Goi M (1992) Growth of tissue cultured Spathiphyllum on rockwool in a novel film culture vessel under high CO2. Acta Hort 314:139–146
Tanaka M, Goi M, Higashiura T (1998a) A novel disposable film culture vessel. Acta Hort 226:663–670
Tanaka M, Jinno K, Goi M, Higashiura T (1998b) The use of disposable fluorocarbon polymer film culture vessel in micropropagation. Acta Hort 230:73–80
Tanaka M, Dam TTG, Murakami A (2005) Application of a novel disposable film culture system to photoautotrophic micropropagation of Eucalyptus uro-grandis (Urophylia x grandis). In Vitro Cell Dev Biol Plant 41:173–180
Valero-Aracama C, Wilson SB, Kane ME, Philman NL (2007) Influence of in vitro growth conditions on in vitro and ex vitro photosynthetic rates of easy- and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes. In Vitro Cell Dev Biol Plant 43:237–246
Wilson SB, Heo J, Kubota C, Kozai T (2001) A forced ventilation micropropagation system for photoautotrophic production of sweetpotato plug plantlets in a scaled-up culture Vessel: II. Carbohydrate status. Hort Technol 11:95–99
Xiao Y, Kozai T (2004) Commercial application of a photoautotrophic micropropagation system using large vessels with forced ventilation: plantlet growth and production cost. Hort Sci 39:1387–1391
Xiao Y, Kozai T (2006) In vitro multiplication of statice plantlets using sugar-free media. Sci Hort 109:71–77
Xiao Y, Lok Y, Kozai T (2003) Photoautotrophic growth of sugarcane in vitro as affected by photosynthetic photon flux and vessel air exchanges. In Vitro Cell Dev Plant 39:186–192
Xiao Y, He L, Liu T, Yang Y (2005) Growth promotion of gerbera plantlets in large vessels by using photoautotrophic micropropagation system with forced ventilation. Propagat Ornament Plants 5:179–185
Xiao Y, Zhang Y, Dang K, Wang D (2007) Growth and photosynthesis of Dendrobium candidum plantlets cultured photoautotrophically. Propagat Ornamental Plants 7:89–96
Zhang M, Zhao D, Ma Z, Li X, Xiao Y (2009) Growth and photosynthethetic capability of Momordica grosvenori plantlets grown photoautotrophically in response to light intensity. Hort Sci 44:757–763
Zobayed SMA, Kubota C, Kozai T (1999) Development of a forced ventilation micropropagation system for large-scale photoautotrophic culture and its utilization in sweet potato. In Vitro Cell Dev Biol 34:350–355
Zobayed SMA, Afreen F, Kubota C, Kozai T (2000a) Mass propagation of Eucalyptus camaldulensis in a scaled-up vessel under in vitro photoautotrophic condition. Ann Bot 85:587–592
Zobayed SMA, Afreen F, Kubota C, Kozai T (2000b) Evolution of culture vessel for micropropagation: from test tube to culture room. In: Kubota C, Chun C (eds) Transplant production in the 21st century. Kluwer Academic publishers, Dordrecht, pp 3–19
Zobayed SMA, Afreen F, Kozai T (2001) Physiology of eucalyptus plantlets cultured photoautotrophically under forced ventilation. In Vitro Cell Dev Biol Plant 37:807–813
Zobayed SMA, Afreen F, Xiao Y, Kozai T (2004) Recent advancement in research on photoautotrophic micropropagation using large culture vessels with forced ventilation. In Vitro Cell Dev Biol Plant 40:450–458
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Xiao, Y., Niu, G. & Kozai, T. Development and application of photoautotrophic micropropagation plant system. Plant Cell Tiss Organ Cult 105, 149–158 (2011). https://doi.org/10.1007/s11240-010-9863-9
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DOI: https://doi.org/10.1007/s11240-010-9863-9