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Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant

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Abstract

A simple and efficient micropropagation system was developed for Cotoneaster wilsonii through node and shoot tip explants obtained from mature field-grown plants. Of the two explants, node explants were found to be the most effective for axillary shoot proliferation. The highest frequency of shoot induction was achieved when nodal explants were incubated on Murashige and Skoog (MS) medium supplemented with 0.5 mg L−1 thidiazuron (TDZ) and 0.1 mg L−1 α- naphthaleneacetic acid (NAA) with an average of 34 shoots per explant. The microshoots were separated from the multiple shoots and subcultured on MS medium supplemented with 3% (w/v) sucrose and 0.8% (w/v) agar for further shoot growth. Maximum rooting was obtained on half-strength MS medium supplemented with 0.5 mg L−1 indole-3-butyric acid (IBA). The in vitro-grown plantlets were successfully acclimatized in a glasshouse with 98% of survival. High concentrations of TDZ (1.5–2.0 mg L−1) and repeated subcultures resulted hyperhydric shoots. Supplementation of the culture medium with silicon significantly reduced the induction of hyperhydric shoots. Increasing silicon concentration significantly decreased malondialdehyde content of the regenerated shoots. Data indicate that addition of silicon to the culture medium can effectively control hyperhydricity.

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Abbreviations

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

EDXA:

Energy Dispersive X-ray Analysis

MDA:

Malondialdehyde

MS:

Murashige and Skoog medium

NAA:

α- Naphthaleneacetic acid

SEM:

Scanning Electron Microscopy

TBA:

Thiobarbituric acid

TDZ:

Thidiazuron

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Acknowledgments

This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. Project No: 109096-5. Iyyakkannu Sivanesan and Ju Yeon Song were supported by a scholarship from the BK21 Program, the Ministry of Education, Science and Technology, Korea.

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Authors and Affiliations

  1. Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University, Jinju, 660-701, Korea

    Iyyakkannu Sivanesan, Ju Yeon Song & Byoung Ryong Jeong

  2. Department of Horticulture, Gyeongsang National University, Jinju, 660-701, Korea

    Seung Jae Hwang

  3. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660-701, Korea

    Byoung Ryong Jeong

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  1. Iyyakkannu Sivanesan
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  2. Ju Yeon Song
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  3. Seung Jae Hwang
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  4. Byoung Ryong Jeong
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Correspondence to Byoung Ryong Jeong.

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Sivanesan, I., Song, J.Y., Hwang, S.J. et al. Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant. Plant Cell Tiss Organ Cult 105, 55–63 (2011). https://doi.org/10.1007/s11240-010-9841-2

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  • DOI: https://doi.org/10.1007/s11240-010-9841-2

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