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Direct regeneration of protocorm-like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae)

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Abstract

The present study describes the direct regeneration of protocorm-like bodies (PLBs) in leaf explants of the tropical species Oncidium flexuosum. The explants were inoculated in a solid, modified Murashige and Skoog (MS) medium with different concentrations of the growth regulator thidiazuron (TDZ) and with or without 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid (NAA), and kept away from light or in a 16-h photoperiod. The presence of auxins, 2,4-D, and NAA inhibited the formation of PLBs. The highest frequency of explants that regenerated PLBs (80%) was obtained when they were maintained in a culture medium containing 1.5 μM TDZ under dark conditions. In the same culture medium but under a 16-h photoperiod, 95% of the leaf explants presented necrosis. Therefore, darkness was crucial for the regeneration of PLBs in O. flexuosum leaf explants, which is in disagreement with the literature. PLBs developed from the division of epidermal and subepidermal cells mainly on the adaxial side of the apex region of the explant. Plants with well-developed leaves and roots grew after the PLBs were transferred to growth regulator-free medium under a 16-h photoperiod.

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Acknowledgments

We are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the grants and Mrs. Marli K. M. Soares for her technical assistance. This work is part of the PhD thesis of Juliana Lischka Sampaio Mayer (Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Brazil).

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Authors and Affiliations

  1. Biological Sciences Department, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, P.O. Box 09, 13418-900, Piracicaba, São Paulo, Brazil

    Juliana Lischka Sampaio Mayer & Beatriz Appezzato-Da-Glória

  2. Horticultural Center, Instituto Agronômico, P.O. Box 28, 13012-970, Campinas, São Paulo, Brazil

    Giulio Cesare Stancato

Authors
  1. Juliana Lischka Sampaio Mayer
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  2. Giulio Cesare Stancato
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  3. Beatriz Appezzato-Da-Glória
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Correspondence to Beatriz Appezzato-Da-Glória.

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Mayer, J.L.S., Stancato, G.C. & Appezzato-Da-Glória, B. Direct regeneration of protocorm-like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae). Plant Cell Tiss Organ Cult 103, 411–416 (2010). https://doi.org/10.1007/s11240-010-9782-9

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  • DOI: https://doi.org/10.1007/s11240-010-9782-9

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