Abstract
The objective of this study was to determine the effects of different light-emitting diode (LED) light sources on the growth of upland cotton (Gossypium hirsutum L.) plantlets. Shoot bud apex cuttings of upland cotton (1.0 cm) were transplanted on Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/l 6-benzyladenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA) and cultured in vitro for 45 days. They were exposed to 50 μmol m−2 s−1 photosynthetic photon flux (PPF) and a 12-h photoperiod under six different lights: fluorescent lamp (CON), monochromatic blue LED (B), three blue and red LED mixtures (B:R = 3:1, 1:1, 1:3) and monochromatic red LED (R). The effects of the six light sources on growth and morphogenesis of upland cotton plantlets grown in vitro were investigated. Fresh weight, dry weight, stem length and second internode length were greatest in plantlets cultured under the B:R = 1:1 blue and red LED light, followed by blue LED light, and they were lowest in plantlets cultured under a fluorescent lamp. Chlorophyll content, leaf thickness, palisade tissue length, leaf and stomata area were highest in plantlets cultured under blue LED light. Root activity, sucrose, starch and soluble sugar contents were highest in plantlets cultured under red LED light. Our results showed that larger, healthier plantlets and a greater biomass of upland cotton were produced in the presence of red LED supplemented with a quantity of blue LED light. Blue and red LED (B:R = 1:1) was the most suitable light for the growth of upland cotton plantlets in vitro, and it may be used as alternative light source for an upland cotton culture system.
Similar content being viewed by others
References
Anzelika K, Renata MC, Stase D, Silva Z, Genadij K, Gintautas T, Pavelas D, Arturas Z (2008) In vitro culture of Chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 3:161–167
Arnon DL (1949) Copper enzymer in isolated chloroplast polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15
Aydin Y, Ipekci Z, Talasora T, Zehir A, Bajrovic K, Gozukirmizi N (2004) High frequency somatic embryogenesis in cotton. Biol Plant 48:491–495
Bicakci E, Memon AR (2005) An efficient and rapid in vitro regeneration system for metal resistant cotton. Biol Plant 49:415–417
Bourget CM (2008) An introduction to light-emitting diodes. HortScience 43:1944–1946
Briggs WR, Olney MA (2001) Photoreceptors in plant photomorphogenesis to date, five photochromes, two cryptochrome, one phototropin and one superchrome. Plant Physiol 125:85–88
Briggs WR, Beck CF, Cashmore AR, Christie JM, Hunghes J (2001) The phototropin family of photoreceptors. Plant Cell 13:993–997
Clouse SD (2001) Integration of light and brassinosteroid signals in etiolated seedling growth. Trends Plant Sci 6:443–445
Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2007) Flowering of Euphorbia millii plantlets in vitro as affected by paclobutrazol, light emitting diodes (LEDs) and sucrose. Acta Hort 764:169–173
Doi M, Shigenaga A, Emi T, Kinoshita T, Shimazaki K (2004) A transgene encoding a blue-light receptor, phot1, restores blue light responses in the Arabidopsis phot1phot2 double mutant. J Exp Bot 55:517–523
Duong TN, Hong LTA, Watanabe H, Goi M, Tanaka M (2003) Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hort 616:121–127
Felker FC, Doehlert DC, Eskins K (1995) Effects of red and blue light on the composition and morphology of maize kernels grown in vitro. Plant Cell Tissue Org Cult 42:147–152
Hahn EJ, Bae CH, Lee YB (1998) Growth and leaf-surface characteristics of chrysanthemum plantlets between micropropagation and microponic system. J Kor Soc Hort Sci 39:838–842
Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affects in vitro growth of Rehmannia glutinose plantlets. Plant Biol 43:247–250
Heo JW, Lee CW, Chakrabarty D, Paek KY (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light emitting diode (LED). Plant Growth Regul 38:225–230
Hoenecke ME, Bula RJ, Tibbits TW (1992) Importance of blue photon levels for lettuce seedlings grown under red-light-emitting diodes. HortScience 27:427–430
Jao RC, Fang W (2004) Effects of frequency and duty ratio on the growth of potato plantlets in vitro using light-emitting diodes. HortScience 39:375–379
Jao RC, Lai CC, Fang W, Chang SF (2005) Effects of red light on the growth of Zantedeschia plantlets in vitro and tuber formation using light-emitting diodes. HortScience 40:436–438
Jin S, Zhang X, Nie Y, Guo X, Liang S, Zhu H (2006) Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton. Biol Plant 50:519–524
Kevin W (2000) ‘Photo-Manipulation-Boxes’: an instrument for the study of plant photobiology. Plant Photobiol 26:3–15
Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of Chrysanthemum plantlets in vitro. Sci Hort 101:143–151
Kinoshita T, Doi M, Suetsugu N, Kagawa T, Wada M, Shimazaki K (2001) Phot1 and phot2 mediate blue light regulation of stomatal opening. Nature 414:656–660
Kowallik W (1982) Blue light effects on respiration. Plant Physiol 33:51–72
Kraepiel Y, Mipiniac E (1997) Photomorphogenesis and phytohormones. Plant Cell Environ 20:807–812
Lee AE, Tewari RK, Hahn EJ, Paek KY (2007) Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania somnifera (L.) Dunal. plantlets. Plant Cell Tissue Organ Cult 90:141–151
Lian ML, Murthy HN, Paek KY (2002) Effect of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ’Pesaro’. Sci Hort 94:365–370
Martin AB, Cuadrado Y, Guerra H, Gallego P, Hita O, Martine L, Dorado A, Villalobos N (2000) Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and nonembrogenic calli from Medicago arborea L. Plant Sci 154:143–151
Massa GD, Kim HH, Wheeler RM, Mitchell CA (2008) Plant productivity in response to LED lighting. HortScience 43:1951–1956
Mithilesh K, Bakesh T (2001) Plant regeneration in cotton: a short-term inositol starvation promotes developmental synchrony in somatic embryogenesis. In Vitro Cell Dev Biol Plant 40:294–298
Miyashita Y, Kitaya Y, Kozai T, Kimura T (1995) Effects of red and far-red light on the growth and morphology of potato plantlets in vitro: using light emitting diode as a light source for micropropagation. Acta Hort 393:189–194
Morrow RC (2008) LED lighting in horticulture. HortScience 43:1947–1950
Mortensen LM, Stromme E (1987) Effects of light quality on some greenhouse crops. Sci Hort 33:27–36
Moshe R, Dalia E (2007) On the effect of light on shoot regeneration in petunia. Plant Cell Tissue Org Cult 89:49–54
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Nhut DT, Takamura T, Watanabe H, Murakami A, Murakami K, Tanaka M (2002) Sugar-free micropropagation of Eucalyptus citriodora using light-emitting diode (LEDs) and film-rockwool culture system. Environ Control Biol 40:147–155
Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tissue Org Cult 73:43–52
Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2005) Artificial light source using light-emitting diodes (LEDs) in the efficient micropropagation of Spathiphyllum plantlets. Acta Hort 692:137–142
Outlaw WH Jr (2003) Integration of cellular and physiological functions of guard cells. CRC Crit Rev Plant Sci 22:503–529
Ozyigit II, Gozukirmizi N (2009) Efficient shoot and root formation from cotton shoot apices. Ru J Plant Physio 56:527–531
Puspa RP, Ikuo K, Ryosuke M (2008) Effect of red-and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tissue Org Cult 92:147–153
Saebo A, Krekling T, Appelgren M (1995) Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tissue Org Cult 41:177–185
Sakhanokho HF, Peggy OA, May OL, Chee PW (2005) Putrescine enhances somatic embryogenesis and plant regeneration in upland cotton. Plant Cell Tissue Org Cult 81:91–95
Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsium annuum L.) grown under red light emitting diodes supplemented with blue or far-red light. Ann Bota 79:273–282
Shimazaki K, Doy M, Assmann SM, Kinoshita T (2007) Light regulation of stomatal movement. Annu Rev Plant Biol 58:219–247
Sims DA, Pearcy RW (1992) Response of leaf anatomy and photosynthetic capacity in Alocasia macrorrhiza (Araceae) to a transfer from low to high light. Am J Bot 79:449–455
Sujatha M, Sailaja M (2005) Stable genetic transformation of castor (Ricinus communis L.) via Agrobacterium tumefaciens-mediated gene transfer using embryo axes from mature seeds. Plant Cell Rep 23:803–810
Sulekha H, Anuradhav K, Satishm N, Anjank B, Dineshc A, Kazav K (2000) Influence of explants, genotypes and culture vessels on sprouting and proliferation of pre-existing meristems of cotton (Gossypium hirsutum L. and Gossypium arboreum L.). In vitro Cell Dev Biol Plant 36:505–510
Sun YQ, Zhang XL, Huang C, Guo XP, Nie YC (2006) Somatic embryogenesis and plant regeneration from different wild diploid cotton (Gossypium) species. Plant Cell Rep 25:289–296
Sun JY, Li WM, Zhang HS, Zhao JL, Yin XL, Wang L (2009) Somatic embryogenesis and plant regeneration in glandless upland cotton (Gossypium hirsutum L.). Front Agric China 3:279–283
Takahashi K, Fujino K, Kikuta Y, Koda Y (1995) Involvement of the accumulation of sucrose and the synthesis of cell wall polysaccharides in the expansion of potato cells in response to jasmonic acid. Plant Sci 111:11–18
Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth of Cymbidium plantlets cultured under super bright and blue light-emitting diodes (LEDs). J Hort Sci Biotech 73:39–44
Tanreer K, Akii KS, Pant RC (2006) Regeneration via somatic embryogenesis and organogenesis in different cultivars of cotton (Gossypium spp.). In Vitro Cell Dev Biol Plant 42:498–501
Tripathy BC, Brown CS (1995) Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiol 107:407–411
Wang QY (1982) Microscope technology of plant. Plant Teaching and Research Group of Nanjing Agricultural University, Nanjing (in Chinese)
Wongnok A, Piluek C, Techasilpitak T, Tantivivat S (2008) Effects of light emitting diodes on micropropagation of Phalaenopsis orchids. Acta Hort 788:149–156
Zeng B, Wang QY, Tang CM (2008) Anatomic analysis on heterosis in three transgenic bt pest-resistant hybrid cotton (G. hirsutum L.). Acta Agron Sin 34:496–505 (in Chinese)
Zhang BH, Liu F, Liu ZH, Wang HM, Yao CB (2001) Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Plant Growth Reg 33:137–149
Zhang YS, Huang X, Chen YF (2009) Experimental course of plant physiology. Higher Education Press, Beijing (in Chinese)
Zhao HM, Ai HL, Wei CY (2007) Effects of hygromyc in on cotton cultures and its application in agrobacterium-mediated cotton transformation. In Vitro Cell Dev Biol Plant 43:111–118
Zhu SW, Sun JS (2000) Rapid plant regeneration from cotton (Gossypium hirsutum L.). Chin Sci Bull 45:1171–1174
Acknowledgments
We gratefully acknowledge the technical assistance of Prof Oingya Wang for help in making cross-sections of upland cotton leaves. This work was supported by a grant from 863 National High Technology Program of China (No: 2006AA03A165) and Natural Science Foundation of China (No: 30972035).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Li, H., Xu, Z. & Tang, C. Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell Tiss Organ Cult 103, 155–163 (2010). https://doi.org/10.1007/s11240-010-9763-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11240-010-9763-z