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An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

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Abstract

Regrowth of encapsulated microshoots, using alginate encapsulation, of Cineraria maritima reached 82.35% following 6 months of storage. Amongst developing plantlets, 33.33% exhibited formation of multiple shoots at the onset of regrowth and 11.76% demonstrated simultaneous formation of shoots and roots. Healthy root formation was observed in plantlets following 2 weeks of their transfer to half-strength Murashige and Skoog medium containing 1.0 mg l−1 α-naphthalene acetic acid. Plants were transplanted to the greenhouse in three batches with 90% frequency of survival. Molecular analysis of randomly selected plants from each batch was conducted using 20 random amplified polymorphic DNA (RAPD) markers. Of 20 primers tested, 14 produced amplification products, and a total of 69 bands with an average of 4.93 bands per primer were observed. Of these 69 scorable bands, only 20% of bands were polymorphic. Cluster analysis of the RAPD profiles revealed an average similarity coefficient of 0.944 thus confirming molecular stability of plants derived from encapsulated microshoots following 6 months of storage.

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Abbreviations

BA:

Benzyladenine

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthalene acetic acid

UPGMA:

Unweighted pair grouping method with arithmetic-mean

RAPD:

Randomly amplified polymorphic DNA

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Acknowledgments

The authors wish to express their sincere thanks to the Director, CIMAP, for providing the facilities to carry out this research. Heartfelt thanks are also due to Dr. G. D. Bagchi (Botany and Pharmacognosy Division) and Dr. A. Samad (Plant Pathology Division) for their kind help and cooperation. Profound appreciation also goes to the photography Division of the institute. The financial assistance to the senior author from Council of Scientific and Industrial Research (CSIR, India) in the form of fellowship is gratefully acknowledged.

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  1. Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CSIR), Lucknow, 226015, India

    Vikas Srivastava, Shamshad A. Khan & Suchitra Banerjee

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  1. Vikas Srivastava
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  2. Shamshad A. Khan
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  3. Suchitra Banerjee
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Correspondence to Suchitra Banerjee.

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Srivastava, V., Khan, S.A. & Banerjee, S. An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage. Plant Cell Tiss Organ Cult 99, 193–198 (2009). https://doi.org/10.1007/s11240-009-9593-z

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  • DOI: https://doi.org/10.1007/s11240-009-9593-z

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