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Protoplast isolation and plant regeneration of different genotypes of Petunia and Calibrachoa

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Abstract

Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.

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Abbreviations

BA:

6-Benzyladenine

FW:

Fresh weight

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog (1962)

NAA:

1-Naphthaleneacetic acid

TDZ:

Thidiazuron

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Acknowledgments

This project was funded by the Federal Ministry of Economy and Technology (BMWi). The authors thank Dr. Andrea Dohm, breeding director Selecta Klemm GmbH, for excellent cooperation during this study, and Professor Errol Hewett (Massey University, Palmerston North, New Zealand) for linguistic editing of the manuscript.

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Authors and Affiliations

  1. Institute of Ornamental and Woody Plant Science, Leibniz University Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany

    L. Meyer, M. Serek & T. Winkelmann

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  1. L. Meyer
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  2. M. Serek
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  3. T. Winkelmann
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Correspondence to L. Meyer.

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Meyer, L., Serek, M. & Winkelmann, T. Protoplast isolation and plant regeneration of different genotypes of Petunia and Calibrachoa . Plant Cell Tiss Organ Cult 99, 27–34 (2009). https://doi.org/10.1007/s11240-009-9572-4

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  • DOI: https://doi.org/10.1007/s11240-009-9572-4

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