Skip to main content
SpringerLink
Log in

In vitro separation of a rose chimera

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

“Fairmount 1 thorny” (“FM1 thorny”) (a Rosa multiflora Thunb ex. J. Murr.) and a thornless sport of “FM1 thorny” (“Fairmount 1” (“FM1”)) were established in vitro to investigate chimeral segregation under various levels of BA and to obtain a pure thornless rose. While the chimeral thornless sport was expected to segregate in vitro and yield both thorny and thornless plantlets, “FM1 thorny” was to yield only thorny plants. “FM1” segregated in vitro into its constituent genotypes and yielded thorny and thornless plantlets, suggesting that “FM1” is chimeral. “FM1 thorny” produced only thorny plants in vitro. These results indicate that the “FM1 thorny” clone was not chimeral (pure thorny) and that the thornless regenerates of “FM1” did not develop via somaclonal variation. There was a significant linear relationship between increasing BA concentration and the percentage of thorny plants. Among a population of 690 tissue culture derived plants from all the BA experiments, 6 plants were classified as pure thornless plants 1 year later.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

BA:

6-benzylaminopurine

MS:

Murashige and Skoog

NAA:

Naphthaleneacetic acid

References

  • Arene L, Pellegrino C, Gudin S (1993) A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues. Euphytica 71:83–90. doi:10.1007/BF00023470

    Article  Google Scholar 

  • Burger DW, Liu L, Zary KW, Lee CI (1990) Organogenesis and plant regeneration from immature embryos of R. hybrida L. Plant Cell Tissue Organ Cult 21:147–152. doi:10.1007/BF00033434

    Article  Google Scholar 

  • Canli FA (2003) A review on thornless roses. Pak J Biol Sci 19:1712–1719

    Google Scholar 

  • Canli FA, Skirvin RM (2003) Separation of thornless rose chimeras into their (Rosa sp.) consistent genotypes in vitro. Pak J Biol Sci 19:1644–1648

    Google Scholar 

  • Compas PS, Pais MSS (1990) Mass propagation of the dwarf rose cultivar “Rosamini”. Sci Hortic (Amsterdam) 43:321–330. doi:10.1016/0304-4238(90)90103-L

    Article  Google Scholar 

  • Druitt L, Shoup M (1991) Thornless roses. Horticulture 69:78–82

    Google Scholar 

  • Firoozabady E, Moy Y, Courtney-Gutterson N, Robinson K (1994) Regeneration of transgenic rose (Rosa hybrida) plants from embryogenic tissue. Bio-Technology 12:611–613

    Google Scholar 

  • Hall HK, Quazi H, Skirvin RM (1986) Isolation of a pure thornless “Loganberry” by meristem tip culture. Euphytica 35:1039–1044. doi:10.1007/BF00028616

    Article  Google Scholar 

  • Hsia C, Korban S (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima. Plant Cell Tissue Organ Cult 44:1–6. doi:10.1007/BF00045906

    Article  CAS  Google Scholar 

  • Jabbarzadeh Z, Khosh-Khui M (2005) Factors effecting tissue culture of Damask rose (Rosa Damascena Mill.). Sci Hortic (Amsterdam) 105:475–482. doi:10.1016/j.scienta.2005.02.014

    Article  CAS  Google Scholar 

  • Khosh-Khui M, Sink KC (1982) Micropropagation of new and old world rose species. J Hortic Sci 57:315–319

    Google Scholar 

  • Kim CK, Chung JD, Jee SK, Oh JY (2003) Somatic embryogenesis from in vitro grown leaf explants of Rosa hybrida L. J Plant Biotechnol 5:161–164

    Article  Google Scholar 

  • Kumar A, Sood A, Plani UT, Gupta AK, Plani LMS (2001) Micropropagation of Rosa Damascena Mill. from mature bushes using thidiazuron. J Hortic Sci Biotechnol 76:30–34

    CAS  Google Scholar 

  • Kunitake H, Imamizo H, Mii M (1993) Somatic embryogenesis and plant regeneration from immature seed-derived calli of rose (Rosa rugosa Thunb). Plant Sci 90:187–194. doi:10.1016/0168-9452(93)90239-V

    Article  CAS  Google Scholar 

  • Lloyd D, Roberts AV, Short KC (1988) The induction in vitro adventitious shoots in Rosa. Euphytica 37:31–36. doi:10.1007/BF00037220

    Article  Google Scholar 

  • McPheeters KD, Skirvin RM (1983) Histogenic layer manipulation in chimeral “Thornless Evergreen” trailing blackberry. Euphytica 32:351–360. doi:10.1007/BF00021443

    Article  Google Scholar 

  • McPheeters KD, Skirvin RM (1989) Somaclonal variation among ex vitro “Thornless Evergreen” trailing blackberries. Euphytica 42:155–162. doi:10.1007/BF00042627

    Article  Google Scholar 

  • Morey D (1969) Selection criteria for breeding. In: Mastalerz JW, Langhans RW (eds) Roses, Pennsylvania flower growers. New York State flower grower association Inc, New York

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Nobbs KJ (1984) Breeding thornless roses. American rose annual. The American Rose Society, Shreveport, LA

    Google Scholar 

  • Noriega C, Sondahl MR (1991) Somatic embryogenesis in hybrid tea roses. Bio-Technology 9:991–993

    Google Scholar 

  • Oliver WG (1986) A precis of thornless development. The rose. R Natl Rose Soc 80:3

    Google Scholar 

  • Pati PK, Sharma M, Sood A, Ahuja PS (2004) Direct shoot regeneration from leaf explants of Rosa damascene Mill. In Vitro Cell Dev Biol Plant 40:192–195. doi:10.1079/IVP2003503

    Article  Google Scholar 

  • Rosu A, Skirvin RM, Bein A, Norton MA, Kushad M (1995) The development of putative adventitious shoots from a chimeral thornless rose (Rosa multiflora Thunb. ex J. Murr.) in vitro. J Hortic Sci 70:901–907

    Google Scholar 

  • Short KC, Roberts AV (1991) Rosa spp. (roses): in vitro culture, micropropagation and production of secondary products. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, medical and aromatic plants, vol 15. Springer-Verlag, Berlin, pp 376–397

    Google Scholar 

  • Skirvin RM, Chu MC (1979) In vitro culture of “Forever Yours” rose. HortScience 14:608–610

    Google Scholar 

  • Skirvin RM, Chu MC, Young HJ (1990) Rose. In: Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture. Ornamental species, vol 5. McGraw Hill Publishing Co, New York, pp 716–743

    Google Scholar 

  • Staba JE (1969) Plant tissue culture as a technique for the phytochemist. Recent Adv Phytochem 2:80

    Google Scholar 

  • Tweddle D, Roberts AV, Short KC (1984) In vitro culture of roses. In: Novak FJ, Havel L, Doleze J (eds) Plant tissue and cell culture application to crop improvement. Czechoslovak Academy of Sciences, Prague, pp 529–530

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticulture, Faculty of Agriculture, Suleyman Demirel University, Cunur, Isparta, Turkey

    Fatih A. Canli

  2. University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Robert M. Skirvin

Authors
  1. Fatih A. Canli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert M. Skirvin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fatih A. Canli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Canli, F.A., Skirvin, R.M. In vitro separation of a rose chimera. Plant Cell Tiss Organ Cult 95, 353–361 (2008). https://doi.org/10.1007/s11240-008-9449-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-008-9449-y

Keywords

Search

Navigation