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Antioxidant response of Nicotiana tabacum cv. Bright Yellow 2 cells to cadmium and nickel stress

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Abstract

Plant cell cultures are a suitable model system for investigation of the physiological mechanisms of tolerance to environmental stress. We have determined the effects of Cd (0.1 and 0.2 mM CdCl2) and Ni (0.075 and 0.75 mM NiCl2) on Nicotiana tabacum L. cv. Bright Yellow (TBY-2) cell suspension cultures over a 72-h period. Inhibition of growth, loss of cell viability and lipid peroxidation occurred, in general, only when the TBY-2 cells were grown at 0.2 mM CdCl2 and at 0.75 mM NiCl2. At 0.1 mM CdCl2, a significant increase in growth was determined at the end of the experiment. Increases in the activities of all of the four enzymatic antioxidant defence systems tested, were induced by the two concentrations of Cd and Ni, but at different times during the period of metal exposure. Overall, the cellular antioxidant responses to Cd and Ni were similar and were apparently sufficient to avoid oxidative stress at the lower concentrations of Cd and Ni. The activities of glutathione reductase and glutathione S-transferase increased early but transiently, whereas the activities of catalase and guaiacol peroxidase increased in the latter half of the experimental period. Therefore it is likely that the metabolism of reduced glutathione was enhanced during the initial onset of the stress, while catalase and guaiacol-type peroxidase appeared to play a more important role in the antioxidant response once the stress became severe.

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Abbreviations

Cd:

Cadmium

Ni:

Nickel

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

APX:

Ascorbate peroxidase

CAT:

Catalase

GPOX:

Guaiacol-type peroxidase

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

GR:

Glutathione reductase

EDTA:

Ethylenediaminetetracetic acid

TBARS:

Thiobarbituric acid reactive substances

MDA:

Malondialdehyde

GST:

Glutathione S-transferases

DHAR:

Dehydroascorbate reductase

MDHAR:

Monodehydroascorbate reductase

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Acknowledgments

This work was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP—Grant no. 04/08444-6). R.A.A. and P.L.G. would like to thank the Conselho nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil) for the fellowship and scholarship granted. C.C.M. would like to thank FAPESP for the scholarship. P.L.G. and G.B.P. contributed equally to the research.

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Authors and Affiliations

  1. Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (USP), Piracicaba, 13418-900, SP, Brazil

    Priscila L. Gratão, Flávia R. Capaldi & Ricardo A. Azevedo

  2. Laboratório de Ecotoxicologia, Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, 13416-700, SP, Brazil

    Georgia B. Pompeu

  3. Laboratório de Biologia Celular e Molecular, Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, 13416-700, SP, Brazil

    Victor A. Vitorello

  4. Department of Biological Sciences, University of Lancaster, Lancaster, LA1 4YQ, UK

    Peter J. Lea

Authors
  1. Priscila L. Gratão
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  2. Georgia B. Pompeu
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  3. Flávia R. Capaldi
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  4. Victor A. Vitorello
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  5. Peter J. Lea
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  6. Ricardo A. Azevedo
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Correspondence to Ricardo A. Azevedo.

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Gratão, P.L., Pompeu, G.B., Capaldi, F.R. et al. Antioxidant response of Nicotiana tabacum cv. Bright Yellow 2 cells to cadmium and nickel stress. Plant Cell Tiss Organ Cult 94, 73–83 (2008). https://doi.org/10.1007/s11240-008-9389-6

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