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In vitro propagation of Helleborus species

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Abstract

A general in vitro cloning system was established for four Helleborus species: H. argutifolius, H. foetidus, H. niger and H. orientalis. The plant material was introduced in vitro from axillary buds. A Murashige and Skoog (MS)—based medium (Murashige and Skoog 1962) was used supplemented with 2% (w/v) sucrose, 2-isopentenyladenine (2-iP) and 6-benzylaminopurine (BA). Multiplication rates depended on the genotype and varied from 1.3 for H. foetidus till 3.8 for H. niger. The first results showed that the rooting phase could be done ex vitro. Rooting was induced by a drench for one week in a solution of indole-3-butyric acid (IBA -3 mg l−1) and 1-naphthaleneacetic acid (NAA-1 mg l−1) at 5°C.

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Abbreviations

BA:

6-benzylaminopurine

IBA:

Indole-3-butyric acid

2-iP:

2-isopentenyladenine

MS:

Murashige and Skoog salts

NAA:

1-naphtaleneacetic acid

NOA:

Naphthoxyacetic acid

PPM™:

Preservative for Plant Tissue Culture Media

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Acknowledgements

The authors thank T. Versluys for the technical supports and ‘het Wilgenbroek’ (Oostkamp, Belgium) for the supply of high quality plants.

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Authors and Affiliations

  1. Department of Plant Production, Genth University, Coupure links 653, 9000, Gent, Belgium

    Emmy Dhooghe & Marie-Christine Van Labeke

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  1. Emmy Dhooghe
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  2. Marie-Christine Van Labeke
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Correspondence to Emmy Dhooghe.

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Dhooghe, E., Van Labeke, MC. In vitro propagation of Helleborus species. Plant Cell Tiss Organ Cult 91, 175–177 (2007). https://doi.org/10.1007/s11240-007-9280-x

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  • DOI: https://doi.org/10.1007/s11240-007-9280-x

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