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Overexpression of a NTR1 in transgenic soybean confers tolerance to water stress

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Abstract

Methyl jasmonate (MeJA) is an important plant regulator that involves in plant development and regulates the expression of plant defense genes in response to various stresses such as wounding, drought, and pathogens. In order to determine the physiological role of endogenous MeJA in plants, a NTR1 from Brassica campestris encoding a jasmonic acid carboxyl methyltransferase that produces methyl jasmonate was constructed under the control of CaMV 35S promoter and transformed into soybean [Glycine max (L) Merrill]. The transgenic soybean plants constitutively expressed the NTR1 and accumulated more MeJA levels than wild type plants. Overexpression of the gene in transgenic soybean conferred tolerance to dehydration during seed germination and seedling growth as reflected by the percentage of the fresh weight of seedlings. In addition, the transgenic soybean plants also conferred better capacity to retain water than wild type plants when drought tolerance was tested using detached leaves.

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Abbreviations

AS:

Acetosyringone

BAP:

6-benzylaminopurine

JA:

Jasmonic acid

MeJA:

Methyl jasmonate

POD:

Peroxidase

PPT:

Phosphinothricin

SOD:

Superoxide dismutase

VSPs:

Vegetative storage proteins

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Acknowledgements

This work was supported by the Shandong Provincial Education Department Foundation (No. J05K04).

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Correspondence to Ren-Gao Xue.

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Xue, RG., Zhang, B. & Xie, HF. Overexpression of a NTR1 in transgenic soybean confers tolerance to water stress. Plant Cell Tiss Organ Cult 89, 177–183 (2007). https://doi.org/10.1007/s11240-007-9231-6

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  • DOI: https://doi.org/10.1007/s11240-007-9231-6

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