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Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures

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Abstract

A system for growing in liquid medium whole plants of Valeriana glechomifolia, endemic to southern Brazil and capable of accumulating bioactive valepotriates, is described. Murashige and Skoog (MS) and Gamborg B5 (B5) media (1.0×, 0.3× and 0.1× strength) without phytohormones were evaluated after four weeks of culture in relation to growth and valepotriate yield. Plants grown in 1.0× MS displayed greatest growth and valepotriate yields and the study of the light condition showed that plants grown under light and dark had similar weight increase and maximum valepotriate yield, 27.2 mg/g DW and 25.0 mg/g DW, respectively. Valtrate was the most abundant valepotriate, followed by acevaltrate and didrovaltrate.

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Abbreviations

ACE:

Acevaltrate

DID:

Didrovaltrate

B5:

Gamborg B5 medium

GI:

Growth Index

MS:

Murashige and Skoog medium

VAL:

Valtrate

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Acknowledgements

The authors are grateful to the Brazilian agencies CAPES, CNPq and FAPERGS for financial support. We would also like to thank Prof. Dr. Pedro R. Petrovick (Faculdade de Farmácia, UFRGS) for lending the HPLC equipment.

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Authors and Affiliations

  1. Programa de Pós Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, UFRGS, Caixa Postal 15005, 91501-970, Porto Alegre, Brazil

    Denise Russowski, Natasha Maurmann & Arthur Germano Fett-Neto

  2. Programa de Pós Graduação em Ciências Farmacêuticas, UFRGS, Av. Ipiranga, 2752, 90610-000, Porto Alegre, RS, Brazil

    Sandra Beatriz Rech

Authors
  1. Denise Russowski
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  2. Natasha Maurmann
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  3. Sandra Beatriz Rech
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  4. Arthur Germano Fett-Neto
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Correspondence to Arthur Germano Fett-Neto.

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Russowski, D., Maurmann, N., Rech, S.B. et al. Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures. Plant Cell Tiss Organ Cult 86, 211–218 (2006). https://doi.org/10.1007/s11240-006-9109-z

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  • DOI: https://doi.org/10.1007/s11240-006-9109-z

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