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Interspecific hybrids between Medicago sativa L. and annual Medicago containing Alfafa weevil resistance

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Abstract

Non-embryogenic protoplasts of Medicago rugosa and M. scutellata were electro-fused with iodoacetic acid-treated protoplasts of M. sativa (alfalfa). Putative somatic hybrid callus were obtained and some plants regenerated from both combinations. Hybridity of regenerants was confirmed by morphology, molecular means and cytological observations. Parental specific bands were recognized in somatic hybrids by Southern analysis. The somatic hybrids were perennial and their morphology was similar to M. sativa. Cytological observations were carried out on the somatic hybrids, their vegetative clones and self-pollinated offspring. Original somatic hybrids were aneuploids (2n=31–59), but during vegetative proliferation, their chromosome numbers reduced to 32. Those clones of hybrids formed seeds from M. sativa (+) M. rugosa by self-crossing. Chromosomal rearrangements within the parental genomes were observed in vegetative clones of hybrids and their S1 offspring by Genomic in situ Hybridization (GISH). Some of S1 offspring from M. sativa (+) M. rugosa showed better spring growth than parental M. sativa and tend to be tolerant to Alfalfa weevil. It was considered that these traits were introduced from the genome transferring M.␣rugosa chromosome to M. sativa. The cell fusion may still have a potential in transferring alien chromosomes in order to increase the genetic variation for crop breeding.

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Abbreviations

CTAB:

cetyl trimethyl ammonium bromide

DAPI:

4,6-diamino-2-phenylindole dihydro- chloride

2,4-D :

2,4-dichlorophenoxi-acetic acid

GISH:

Genomic in situ Hybridization

IOA:

iodoac- etamide

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Acknowledgements

Authors thanked Mr. K. Fukaya for his help to this work. This research was supported in part by Ministry of Agriculture, Forestry and Fisheries, Japan.

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Authors and Affiliations

  1. Crop Institute, Aichi Agricultural research Center, 480 1193, Nagakute Aichi, Japan

    Yuko Mizukami, Mitsuru kato, Michio Kanbe & Susumu Inami

  2. Department of Forage Crop Breeding, National Institute of Livestock and Grassland Science, 329 2793, Nishinasuno, Tochigi, Japan

    Tadashi Takamizo

  3. Department of Biological Mechanisms and Function, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, 464 8601, Nagoya, Aichi, Japan

    Yuko Mizukami & Kazumi Hattori

Authors
  1. Yuko Mizukami
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  2. Mitsuru kato
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  3. Tadashi Takamizo
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  4. Michio Kanbe
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  5. Susumu Inami
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  6. Kazumi Hattori
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Correspondence to Yuko Mizukami.

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Mizukami, Y., kato, M., Takamizo, T. et al. Interspecific hybrids between Medicago sativa L. and annual Medicago containing Alfafa weevil resistance. Plant Cell Tiss Organ Cult 84, 80–89 (2006). https://doi.org/10.1007/s11240-005-9008-8

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  • DOI: https://doi.org/10.1007/s11240-005-9008-8

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