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Expression of the glutathione S-transferase gene (NT107) in transgenic Dianthus superbus

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Abstract

Using an Agrobacterium-mediated transformation method based on wounding cultured immature seeds with carborundum (600 mesh) in liquid, auxin-regulated tobacco glutathione S -transferase (GST) (NT107) constructs were used to transform Dianthus superbusL. A 663 bp DNA band was found in the transgenic plant genome by PCR analysis using NT107-1 and NT107-2 primers, and a Southern blot analysis showed that the DIG-labelled GST gene was hybridized to the expected amplified genomic DNA fragment from transgenic D. superbus. An overexpression of NT107 led to a twofold increase in GST-specific activity compared to the non-transgenic control plants, and the GST overexpression plants showed an enhanced acclimatization in the soil. To investigate whether an increased expression of GST could affect the resistance of photosynthesis to environmental stress, these plants were subjected to drought and various light intensities from 100 to 3000 μmol m−2s−1. Copper accumulation and the translocation rate were also analysed in the transgenic lines, and the GST overexpression plants were found to synthesize phytochelatin (PC), which functions by sequestering and detoxifying excess copper ions.

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Abbreviations

BAP:

6-benzylaminopurine

CDNB:

1-chloro-2,4-dinitrobenzene

2,4-d:

2,4-dichloro-phenoxyacetic acid

GST:

glutathione S-transferase

IPTG:

isopropyl B-d-thiogalactopyranoside

NAA:

1-naphthaleneacetic acid

TDZ:

thidiazurone

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Authors and Affiliations

  1. College of Agriculture and Life Science, Kangwon National University, Chunchon, 200-701, Korea

    Jung Dae Lim & Chang Yeon Yu

  2. Department of Applied Life Science, College of Life and Environment Science, Konkuk University, Seoul, 143-701, Korea

    Sang Joon Hahn & Ill Min Chung

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  1. Jung Dae Lim
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  2. Sang Joon Hahn
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  3. Chang Yeon Yu
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  4. Ill Min Chung
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Correspondence to Ill Min Chung.

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These two authors contributed equally to this work

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Lim, J.D., Hahn, S.J., Yu, C.Y. et al. Expression of the glutathione S-transferase gene (NT107) in transgenic Dianthus superbus. Plant Cell Tiss Organ Cult 80, 277–286 (2005). https://doi.org/10.1007/s11240-004-1032-6

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  • DOI: https://doi.org/10.1007/s11240-004-1032-6

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