Abstract
Rapeseed (Brassica napus L.) plants transformed with the acb gene encoding acyl-CoA-binding protein from Arabidopsis thaliana were used to demonstrate the physiological effects of transformation as related to the genetic vector structure. The opportunities and limitations of the genetic engineering technologies in the studies of particular proteins are under discussion.
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Abbreviations
- ACBP:
-
acyl-CoA-binding proteins
- EA:
-
erucic acid
- FA:
-
fatty acid
REFERENCES
Kurochkina, S.D. and Kartel', N.A., Genetic Transformation of Plants, Processes of Recombination and Regulation of Gene Expression in Transgenic Plants, Mol. Gen., Mikrobiol., Virusol., 1998, no. 4, pp. 3–12.
Bourque, J.E., Antisense Strategies for Genetic Manipulations in Plants, Plant Sci., 1995, vol. 105, pp. 125–149.
Pedra, J.H.F., Delu-Filho, N., Pirovani, C.P., Contim, L.A.S., Dewey, R.E., Otoni, W.C., and Fontes, E.P.B., Antisense and Sense Expression of a Sucrose Binding Protein Homologue Gene from Soybean in Transgenic Tobacco Affects Plant Growth and Carbohydrate Partitioning in Leaves, Plant Sci., 2000, vol. 152, pp. 87–98.
Romanov, G.A., Plant Genetic Engineering and the Biosafety Problem, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 343–353 (Russ. J. Plant Physiol., Engl. Transl., pp. 303–311).
Semenyuk, E.G., Problem of Risk Evaluation for Transgenic Plants, Agrokhimiya, 2001, no. 10, pp. 85–96.
Zhukova, V.M., Rekoslavskaya, N.I., Salyaev, R.K., and Yur'eva, O.V., Transformed Plants Obtained by Shoot Regeneration after Agrobacterial Infection of Dormant Axillary Buds of Solanum Plants Exemplified by the iaglu Gene, Biotekhnol., 1997, no. 5, pp. 15–21.
Praktikum po biokhimii (Biochemical Mannual), Severin, S.E. and Solov'eva, G.A., Eds., Moscow: Mosk. Gos. Univ., 1989.
Kharchenko, L.N., Method for Determination of Fatty Acid Content in Oil of Oil-Yielding Seeds Using Gas-Liquid Chromatography, Metodicheskie ukazaniya po opredeleniyu biokhimicheskikh pokazatelei kachestva masla i semyan maslichnykh kul'tur (Methodical Recommendation for Determination of Biochemical Indices for Oil and Seed Quality of Oil-Yielding Cultures), Krasnodar: Vses. Nauch. Issled. Inst. Maslich. Kul'tur, 1986, pp. 8–23.
Knudsen, J., Jensen, M.V., Hansen, J.K., Foergeman, N.J., Neergaard, T.B.F., and Gaigg, B., Role of AcylCoA Binding Protein in AcylCoA Transport, Metabolism and Cell Signaling, Mol. Cell. Biochim., 1999, vol. 192, pp. 95–103.
Chye, M.L., Li, H.Y., and Yung, M.H., Single Amino Acid Substitutions at the AcylCoA-Binding Domain Interrupt 14[C]-Palmitoyl-CoA Binding of ACBP2, an Arabidopsis Acyl-CoA-Binding Protein with Ankyrin Repeats, Plant Mol. Biol., 2000, vol. 44, pp. 711–721.
Pacovsky, R.S., Arabidopsis thaliana AcylCoA-Binding Protein: Structure, Functions, Genetics, PhD Dissertation, Michigan: Michigan State Univ., 1996.
Enikeev, A.G., Mishutina, U.O., and Pacovski, R.S., Investigation of Functions of Plant AcylCoA-Binding Proteins by Genetic Engineering Approaches, Vestn. Bashkir. Univ., 2001, no. 2(II), pp. 7–9.
Osipova, G.N., Raps v Sibiri (Rape in Siberia), Novosibirsk: Nauka, 1998.
Goryachkovskaya, T.N., Stepanenko, I.L., Aksenovich, A.V., Anan'ko, E.A., Kolpakov, F.A., Pel'tek, S.E., Kolchanov, N.A., and Shumnyi, V.K., Regulation of Plant Gene Expression: Specification in Data Bases TRRD and GENENET, Mater. Vseros. simp. Izuchenie genoma i geneticheskaya transformatsiya rastenii (Proc. All-Russia Symp. Investigation of the Plant Genome and Genetic Transformation), Novosibirsk: Nauka, 2001, pp. 66–82.
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Translated from Fiziologiya Rastenii, Vol. 52, No. 5, 2005, pp. 751–754.
Original Russian Text Copyright © 2005 by Enikeev, Mishutina.
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Enikeev, A.G., Mishutina, U.O. Physiological Effects of Rapeseed Transformation with the acb Gene as Affected by the Genetic Vector Structure. Russ J Plant Physiol 52, 668–671 (2005). https://doi.org/10.1007/s11183-005-0099-1
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DOI: https://doi.org/10.1007/s11183-005-0099-1