Abstract
Tobacco plants with the introduced desC gene for acyl-lipid Δ9-desaturase from the thermophilic cyanobacterium Synechococcus vulcanus were cultivated on agar-solidified Murashige and Skoog nutrient medium supplemented with ferulic acid and antibiotics at 22°C and a 16-h photoperiod. Control plants were transformed with an empty pGA482 vector. The analysis of fatty acids (FAs) showed that, in transgenic plants, the level of 16:0 and 18:0 FAs decreased substantially, whereas the levels of di- and trienoic FAs increased. Transformed plants were more cold-tolerant. The tolerance to chilling was evaluated from electrolyte leakage from tissues damaged by cold treatments and from the accumulation of a product of lipid peroxidation, malondialdehyde. It was concluded that acyl-lipid Δ9-desaturase was actively expressed in transgenic tobacco plants and converted stearic acid into oleic acid, thus producing a substrate for further synthesis of di- and trienoic FAs. An increased proportion of polyunsaturated FAs in membrane lipids resulted in improved tobacco plant tolerance to chilling.
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Abbreviations
- ACP:
-
acyl-carrying protein
- AOS:
-
antioxidant system
- FA:
-
fatty acid
- MDA:
-
malondialdehyde
- POL:
-
peroxidation of lipids
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Translated from Fiziologiya Rastenii, Vol. 52, No. 5, 2005, pp. 747–750.
Original Russian Text Copyright © 2005 by Popov, Orlova, Kipaikina, Serebriiskaya, Merkulova, Nosov, Trunova, Tsydendambaev, Los.
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Popov, V.N., Orlova, I.V., Kipaikina, N.V. et al. The Effect of Tobacco Plant Transformation with a Gene for Acyl-Lipid Δ9-Desaturase from Synechococcus vulcanus on Plant Chilling Tolerance. Russ J Plant Physiol 52, 664–667 (2005). https://doi.org/10.1007/s11183-005-0098-2
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DOI: https://doi.org/10.1007/s11183-005-0098-2