Abstract
It was shown that, in preparations of winter rye (Secale cereale L.) and winter wheat (Triticum aestivum L.), in proteins immunochemically related to a cold shock protein CSP310, and also in purified CSP310 from winter rye and triticale (Triticosecale X.), nucleic acid was present. Treatments with DNase and RNase showed that this nucleic acid was RNA. This protein-bound RNA was detected in the preparation of constitutively synthesized but not stress-induced protein. Stress-induced CSP310 bound high-molecular RNA in vitro at both 26 and 0°C, but it did not bind DNA. The data obtained permit an assumption that, during low-temperature stress, constitutively synthesized CSP310 with a low uncoupling capacity releases RNA and transits to a stress-induced form with a high uncoupling capacity.
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Abbreviations
- AO:
-
alternative cyanide-resistant oxidase
- CSP:
-
cold shock protein
- NA:
-
nucleic acid
- ssDNA:
-
single-stranded oligodeoxyribonucleotide
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Translated from Fiziologiya Rastenii, Vol. 52, No. 2, 2005, pp. 216–220.
Original Russian Text Copyright © 2005 by Kolesnichenko, Tauson, Zykova, Klimenko, Grabel’nykh, Pobezhimova.
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Kolesnichenko, A.V., Tauson, E.L., Zykova, V.V. et al. Nature of the ligand bound to uncoupling CSP310 protein. Russ J Plant Physiol 52, 189–193 (2005). https://doi.org/10.1007/s11183-005-0028-3
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DOI: https://doi.org/10.1007/s11183-005-0028-3