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Identification of LMW Glutenin-Like Genes from Secale sylvestre Host

  • Plant Genetics
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Abstract

Three low-molecular-weight (LMW) glutenin-like genes (designated as Ssy1, Ssy2, and Ssy3) from Secale sylvestre Host were isolated and characterized. The three genes consist of a predicted highly conservative signal peptide with 20 amino acids, a short N-terminal region with 13 amino acids, a highly variable repetitive domain and a less variable C-terminal domain. The deduced amino acid sequences of the three genes were the LMW-m type due to a methionine residue at the N-terminus. The phylogenetic analysis indicated that the prolamin genes could be perfectly clustered into five groups, including HMW-GS, LMW-GS, α/β-, γ-, and κ-prolamin. The LMW glutenin-like genes of S. sylvestre were more orthologous with the LMW-GS genes of wheat and B hordein genes of barley, which also had been confirmed by the homology analysis with the LMW-GS of wheat at Glu-A3, Glu-B3, and Glu-D3 loci. These results indicated that a chromosome locus (designated as Glu-R3) might be located on the R genome of S. sylvestre with the functions similar to the Glu-3 locus in wheat and its related species.

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  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830, China

    H.-Y. Shang, Y.-M. Wei, H. Long, Z.-H. Yan & Y.-L. Zheng

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__________

From Genetika, Vol. 41, No. 12, 2005, pp. 1656–1664.

Original English Text Copyright © 2005 by Shang, Wei, Long, Yan, Zheng.

The text was submitted by the authors in English.

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Shang, HY., Wei, YM., Long, H. et al. Identification of LMW Glutenin-Like Genes from Secale sylvestre Host. Russ J Genet 41, 1372–1380 (2005). https://doi.org/10.1007/s11177-006-0009-4

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