Abstract
In order to understand sequence information about turtle HMG1 gene, a cDNA encoding HMG1 protein of the Chinese soft-shell turtle (Pelodiscus sinensis) was amplified by RT-PCR from kidney total RNA, and was cloned, sequenced and analyzed. The results revealed that the open reading frame (ORF) of turtle HMG1 cDNA is 606 bp long. The ORF codifies 202 amino acid residues, from which two DNA-binding domains and one polyacidic region are derived. The DNA-binding domains share higher amino acid identity with homologous sequences of chicken (96.5%) and mammals (74%) than homologous sequence of rainbow trout (67%). The polyacidic region shows 84.6% amino acid homology with the equivalent region of chicken HMG1 cDNA. Turtle HMG1 protein contains 3 Cys residues located at completely conserved positions. Conservation in sequence and structure suggests that the functions of turtle HMG1 cDNA may be highly conserved during evolution. To our knowledge, this is the first report of HMG1 cDNA sequence in any reptilian.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Baxevanis, A.D. and Landsman, D., The HMG1 Box Protein Family Classification and Functional Relationships, Nucleic Acids Res., 1995, vol. 23, pp. 1604–1613.
Landsman, D. and Bustin, M., A Signature for the HMG1 Box DNA-Binding Proteins, BioEssays, 1993, vol. 15, pp. 539–546.
Bianchi, M.E., Beltrame, M., and Panonessa, G., Specific Recognition of HMG1 Protein Is Composed of Two Similar Segments (HMG Boxes), Both of Which Have Counterparts in Other Eukaryotic Regulatory Proteins, EMBO J., 1989, vol. 11, pp. 1055–1063.
Bianchi, M.E., Falciola, L., Ferrari, S., and Lilley, D., The DNA-Binding Site of HMG1 Protein Is Composed of Two Similar Segments (HMG Boxes), Both of Which Have Counterparts in Other Eukaryotic Regulatory Proteins, EMBO J., 1989, vol. 11, pp. 1055–1063.
Gaillard, C. and Strauss, F., Association of DNA Fragments Containing polyCApolyTG into Four-Stranded Structures Recognized by HMG1 and HMG2, Science, 1994, vol. 264, pp. 433–436.
Pil, P. and Lippard, S.J., Specific Binding of Chromosomal Protein HMG1 to DNA Damage by the Anticancer Drug Cisplatin, Science, 1992, vol. 256, pp. 234–237.
Javaherian, K., Liu, L.F., and Wang, J.C., Nonhistone Proteins HMG1 and HMG2 Change the DNA Helical Structure, Science, 1978, vol. 199, pp. 1345–1347.
Levy, W.B. and Dixon, G.H., Partial Purification of Transcriptionally Active Nucleosomes from Trout Testis Cells, Nucleic Acids Res., 1979, vol. 5, pp. 4155–4163.
Tremethick, D.J. and Molloy, P.L., Effects of High Mobility Group Proteins 1 and 2 on Initiation and Elongation of Specific Transcription by RNA Polymerase II in Vitro, Nucleic Acids Res., 1988, vol. 16, pp. 11 107–11 123.
Waga, S., Mizuno, S., and Yoshida, M., Chromosomal Protein HMG1 Removes the Transcriptional Block Caused by the Cruciform in Supercoiled DNA, J. Biol. Chem., 1990, vol. 265, pp. 19 424–19 428.
Wen, L., Huang, J.K., Johnson, B.H., and Reeck, G.R., A Human Placental cDNA Clone That Encodes Nonhistone Chromosomal Protein HMG1, Nucleic Acids Res., 1989, vol. 17, pp. 1197–1214.
Yotov, W.V. and St-Arnaud, R., Nucleotide Sequence of a Mouse cDNA Encoding the Nonhistone Chromosomal High Mobility Group Protein 1 (HMG1), Nucleic Acids Res., 1992, vol. 20, p. 3516.
Lee, K.L., Pentecost, B.T., Danna, J.A., et al., Characterization of cDNA Sequence Corresponding to Three Distinct HMG1 mRNA Species in Line CHO Chinese Hamster Cells and Cell Cycle Expression of the HMG1 Gene, Nucleic Acids Res., 1987, vol. 15, pp. 5051–5068.
Paonessa, G., Frank, R., and Cortese, R., Nucleotide Sequence of Rat Liver HMG1 cDNA, Nucleic Acids Res., 1987, vol. 15, p. 9077.
Kaplan, D.J. and Duncan, C.H., Full-Length cDNA Sequence for Bovine High Mobility Group 1 (HMG1) Protein, Nucleic Acids Res., 1988, vol. 16, p. 10 375.
Tsuda, K., Kikuchi, M., Mori, K., et al., Primary Structure of Non-Histone Protein HMG1 Revealed by the Nucleotide Sequence, Biochemistry, 1988, vol. 27, pp. 6159–6163.
Funahashi, J., Sekido, R., Murai, K., et al., δ-Crystallin Enhancer Binding Protein δ EF1 Is a Zinc Finger-Home-odomain Protein Implicated in Ostgastrulation Embryogenesis, Development, 1993, vol. 119, pp. 433–446.
Pentecost, B.T., Wright, J.M., and Dixon, G.H., Isolation and Sequence of cDNAs Coding for a Member of the Family of High Mobility Group Proteins (HMG-T) in Trout and Analysis of HMG-T mRNAs in Trout Tissues, Nucleic Acids Res., 1985, vol. 13, pp. 4871–4888.
Reeck, G.R., Isackson, P.J., and Teller, D.C., Domain Structure in High Molecular Weight High Mobility Group Nonhistone Chromatin Proteins, Nature, 1982, vol. 300, pp. 76–78.
Kohlstaedt, L.A., King, D.S., and Cole, R.D., Native State of High Mobility Group Chromosomal Proteins 1 and 2 Is Rapidly Lost by Oxidation of Sulfhydryl Groups during Storage, J. Biol. Chem., 1986, vol. 25, pp. 4562–4565.
Author information
Authors and Affiliations
Additional information
From Genetika, Vol. 41, No. 7, 2005, pp. 925–930.
Original English Text Copyright © 2005 by Jifang Zheng, Bi Hu, Duansheng Wu.
The text was submitted by the authors in English.
Rights and permissions
About this article
Cite this article
Zheng, J., Hu, B. & Wu, D. Sequence of a cDNA Encoding Turtle High Mobility Group 1 Protein. Russ J Genet 41, 755–759 (2005). https://doi.org/10.1007/s11177-005-0156-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11177-005-0156-z