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Does triploidization produce functional sterility of triploid males of tench Tinca tinca (L.)?

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Abstract

Triploidy interferes with gametogenesis in all fish species tested so far. In fish it results in complete female sterility however, males are still able to develop testis. The reason why sterility levels in triploid fishes differ among species and between sexes is unclear. In the present study the reproductive capacity of triploid males of tench was studied. Flow cytometry revealed sperm cells of triploids to be largely aneuploid with high mosaic DNA, oscillating from haploid DNA to diploid DNA content. Analysis of variance showed an insignificant influence of ploidy level on the percentage of motile spermatozoa, as well as on spermatozoa velocity. Experimental crosses between normal diploid female and triploid males resulted in the appearance of triploid progeny, which exhibited genotypes composed of microsatellite alleles inherited from the founder female and additional allele derived from the donor male. We can conclude that the triploid males analysed in the present study were capable to fertilize eggs derived from diploid females.

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References

  • Benfey TJ (1999) The physiology and behaviour of triploid fishes. Rev Fish Sci 7:39–67

    Article  Google Scholar 

  • Benfey T, Solar I, de Jong G, Donaldson E (1986) Flow-cytometric confirmation of aneuploid in sperm from triploid rainbow trout. Trans Am Fish Soc 115:838–840

    Article  Google Scholar 

  • Collares-Pereira MJ, Madeira JM, Rab P (1995) Spontaneous triploidy in the stone loach Noemacheilus barbatulus (Balitoridae). Copeia 2:483–484

    Article  Google Scholar 

  • Devlin RH, Nagahama Y (2002) Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208:191–364

    Article  CAS  Google Scholar 

  • Flajšhans M, Linhart O, Kvasnička P (1993) Genetic studies of tench (Tinca tinca L.): induced triploidy and tetraploidy and first performance data. Aquaculture 113:301–312

    Article  Google Scholar 

  • Flajšhans M, Rodina M, Halačka K, Vetešnik L, Gela D, Luskova V, Lusk S (2008) Characteristics of sperm of polyploid prussian carp carassius gibelio. J Fish Biol 73:323–328

    Article  Google Scholar 

  • Kohlmann K, Kersten P (2006) PMicrosatellite loci in tench: isolation and variability in a test population. Aquac Int 14:3–7

    Article  CAS  Google Scholar 

  • Lincoln RF (1981) The growth of female and diploid and triploid plaice (Pleuronectes platessa) × flounder (Platichthys flesus) hybrid over one spawning season. Aquaculture 25:259–268

    Article  Google Scholar 

  • Linhart O, Gela D, Flajšhans M, Duda P, Rodina M, Novak V (2000) Alcalase enzyme treatment for elimination of egg stickiness in tench, Tinca tinca L. Aquaculture 191:303–308

    Article  CAS  Google Scholar 

  • Linhart O, Rodina M, Flajšhans M, Mavrodiev N, Nebesarova J, Gela D, Kocour M (2006) Studies on sperm of diploid and triploid tench (Tinca tinca L.). Aquac Int 14:9–25

    Article  Google Scholar 

  • Meirmans PG, Van Tienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794

    Article  Google Scholar 

  • Morishima K, Horie S, Yamaha E, Arai K (2002) A cryptic clonal line of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) evidenced by induced gynogenesis, interspecific hybridization, microsatellite genotyping and multilocus DNA fingerprinting. Zool Sci 19:565–575

    Article  PubMed  Google Scholar 

  • Otto SP, Whitton J (2000) Polyploid incidence and evolution. Annu Rev Genet 34:401–437

    Article  CAS  PubMed  Google Scholar 

  • Rodina M, Cosson J, Gela D, Linhart O (2004) Kurokura solution as immobilization solution for spermatozoa of tench (Tinca tinca L.). Aquac Int 12:119–131

    Article  Google Scholar 

  • Shen J, Fan Z, Wang G (1983) Karyotype studies of male triploid crucian carp (Fangzheng crucian carp) in Heilongjian. Acta Genet Sci 10:133–136

    Google Scholar 

  • Stanley J, Allen SK Jr, Hidu H (1981) Polyploidy induced in the American oyster, Crassostrea virginica with cytochalasin B. Aquaculture 23:1–10

    Article  CAS  Google Scholar 

  • Ueda T, Sawada M, Kobayashi J (1987) Cytogenetical characteristics of embryos between diploid female and triploid male in rainbow trout. Jpn J Genet 62:461–465

    Article  Google Scholar 

  • Vindelov LL, Christensen IJ (1990) Review of techniques and results obtained in one laboratory by an integrated system of methods designed for routine clinical flow cytometric DNA analysis. Cytometry 11:753–770

    Article  CAS  PubMed  Google Scholar 

  • Yamaha E, Murakami M, Hada K, Otani S, Fujimoto T, Tanaka M, Sakao S, Kimura S, Sato S, Arai K (2003) Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of PGC (primordial germ cell)-containing graft. Genetica 119:121–131

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was financial supported by USB RIFCH No: MSM 6007665809 and GACR no. 524/06/0817. Authors give also thanks to two anonymous referees as well as to the Guest Editor for valuable comments on an earlier version of manuscript.

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Authors and Affiliations

  1. Faculty of Fisheries and Protection of Waters, University of South Bohemia, Zatisi 728/II, 389 25, Vodnany, Czech Republic

    Martin Hulak, Vojtech Kaspar, Martin Psenicka, David Gela, Ping Li & Otomar Linhart

  2. Key Laboratory of Freshwater Fish Germplasm Resources and Biotechnology, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, No. 41 Jianghan Road, 434000, Shashi District, Jingzhou City, Hubei, People’s Republic of China

    Ping Li

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  1. Martin Hulak
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  2. Vojtech Kaspar
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  3. Martin Psenicka
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  4. David Gela
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  5. Ping Li
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  6. Otomar Linhart
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Correspondence to Martin Hulak.

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Hulak, M., Kaspar, V., Psenicka, M. et al. Does triploidization produce functional sterility of triploid males of tench Tinca tinca (L.)?. Rev Fish Biol Fisheries 20, 307–315 (2010). https://doi.org/10.1007/s11160-009-9139-9

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  • DOI: https://doi.org/10.1007/s11160-009-9139-9

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