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Isolated Conglutin γ from Lupin, but not Phytate, Lowers Serum Cholesterol Without Influencing Vascular Lesion Development in the ApoE-deficient Mouse Model

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Abstract

Conglutin γ and phytate are considered as potential biofunctional compounds of lupin protein isolate, but their impact on vascular health is unknown. This study aimed to investigate the effect of conglutin γ and phytate, respectively, on circulating levels of sterols, markers of cholesterol biosynthesis and minerals, and on the development and progression of aortic lesions in apoE-deficient mice. To this end, mice were fed a western diet with either casein (200 g/kg; served as a control), conglutin γ from L. angustifolius (200 g/kg) or casein (200 g/kg) supplemented with phytate (5 g/kg) for 16 weeks. Here we found that conglutin γ but not phytate was capable of reducing the circulating concentration of cholesterol. Plasma levels of desmosterol and lathosterol as markers of the cholesterol synthesis were not affected, and 7-dehydrocholesterol was even higher in mice fed conglutin γ than in mice fed casein or casein + phytate. All mice developed pronounced aortic lesions, but histological characterization of plaque area and composition showed no differences between the three groups of mice. Conclusively, conglutin γ exerts cholesterol-lowering effects but appears to have no anti-atherosclerotic properties in the apoE-deficient mice. Phytate neither affected plasma cholesterol nor aortic lesion development.

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Abbreviations

DM:

Dry matter

ICP OES:

Inductively coupled plasma optical emission spectroscopy

L :

Lupinus

25(OH)D3 :

25-hydroxyvitamin D3

7-DHC:

7-dehydrocholesterol

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Acknowledgments

This study was supported by a grant from the Federal Ministry of Education and Research of Germany (01EA1338B).

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Von Danckelmann Platz 2, 06120, Halle (Saale), Germany

    Juliane Radtke, Alexandra Schutkowski, Corinna Brandsch, Frank Hirche & Gabriele I. Stangl

  2. Fraunhofer Institute for Process Engineering and Packaging (Fh-IVV), Freising, Germany

    Katrin Hasenkopf

Authors
  1. Juliane Radtke
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  2. Alexandra Schutkowski
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  3. Corinna Brandsch
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  4. Frank Hirche
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  6. Gabriele I. Stangl
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Correspondence to Gabriele I. Stangl.

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Radtke, J., Schutkowski, A., Brandsch, C. et al. Isolated Conglutin γ from Lupin, but not Phytate, Lowers Serum Cholesterol Without Influencing Vascular Lesion Development in the ApoE-deficient Mouse Model. Plant Foods Hum Nutr 70, 113–118 (2015). https://doi.org/10.1007/s11130-015-0481-x

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