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Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of F 0 fluorescence in leaves of Arabidopsis thaliana and Zea mays

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Abstract

The effect of stepwise increments of red light intensities on pulse-amplitude modulated (PAM) chlorophyll (Chl) fluorescence from leaves of A. thaliana and Z. mays was investigated. Minimum and maximum fluorescence were measured before illumination (F 0 and F M, respectively) and at the end of each light step (\( F^{\prime}_{0} \) and \( F^{\prime}_{\text{M}} \), respectively). Calculated \( F^{\prime}_{0} \) values derived from F 0, F M and \( F^{\prime}_{\text{M}} \) fluorescence according to Oxborough and Baker (1997) were lower than the corresponding measured \( F^{\prime}_{0} \) values. Based on the concept that calculated \( F^{\prime}_{0} \) values are under-estimated because the underlying theory ignores PSI fluorescence, a method was devised to gain relative PSI fluorescence intensities from differences between calculated and measured \( F^{\prime}_{0} \). This method yields fluorometer-specific PSI data as its input data (F 0, F M, \( F^{\prime}_{0} \) and \( F^{\prime}_{\text{M}} \)) depend solely on the spectral properties of the fluorometer used. Under the present conditions, the PSI contribution to F 0 fluorescence was 0.24 in A. thaliana and it was independent on the light acclimation status; the corresponding value was 0.50 in Z. mays. Correction for PSI fluorescence affected Z. mays most: the linear relationship between PSI and PSII photochemical yields was clearly shifted toward the one-to-one proportionality line and maximum electron transport was increased by 50 %. Further, correction for PSI fluorescence increased the PSII reaction center-specific parameter, 1/F 0 − 1/F M, up to 50 % in A. thaliana and up to 400 % in Z. mays.

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Abbreviations

ϕ PSII :

Effective yield for PSII photochemistry

Chl a :

Chlorophyll a

Chl b :

Chlorophyll b

ETR:

Electron transport rate

F 0 :

Minimum fluorescence intensity in the dark-acclimated state

\( F^{\prime}_{0} \) :

Minimum fluorescence intensity in the light-acclimated state

F1 :

Photosystem I fluorescence intensity

F M :

Maximum fluorescence intensity in the dark-acclimated state

\( F^{\prime}_{\text{M}} \) :

Maximum fluorescence intensity in the light-acclimated state

f RC2 :

PSII reaction center-specific factor

F V/F M :

Maximum yield for primary photochemistry of PSII

GH:

Green house-grown

HL:

High-light acclimated

LL:

Low-light grown

PPFD:

Photosynthetic photon flux density

PSI:

Photosystem I

PSII:

Photosystem II

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Acknowledgments

We thank Lars Nichelmann for providing seed and instructions on plant growth. We are grateful to Frank Reichel and Thomas Simon for skillful technical assistance. EP wishes to thank the Heinz Walz GmbH for supporting his research visit at Université Paris-Sud and Gabriel Cornic for fruitful discussions and for creating an inspiring research environment. We are grateful to Dr. Robert Porra for help in preparing this manuscript.

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  1. Erhard E. Pfündel

    Present address: Heinz Walz GmbH, Eichenring 6, 91090, Effeltrich, Germany

Authors and Affiliations

  1. Lehrstuhl für Botanik II der Universität Würzburg, Julius-von-Sachs Institut für Biowissenschaften, 97082, Würzburg, Germany

    Erhard E. Pfündel

  2. Im Biefang 22, 79618, Rheinfelden, Germany

    Christof Klughammer

  3. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraße 3, 06466, Gatersleben, Germany

    Armin Meister

  4. Equipe de Biospectroscopie Végétale, Laboratoire d’Ecologie Systématique et Evolution (UMR 8079) CNRS, Département d’Ecophysiologie Végétale, Université Paris-Sud 11, 91405, Orsay Cedex, France

    Zoran G. Cerovic

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Pfündel, E.E., Klughammer, C., Meister, A. et al. Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of F 0 fluorescence in leaves of Arabidopsis thaliana and Zea mays . Photosynth Res 114, 189–206 (2013). https://doi.org/10.1007/s11120-012-9788-8

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