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Selenium-induced protection of photosynthesis activity in rape (Brassica napus) seedlings subjected to cadmium stress. Fluorescence and EPR measurements

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Abstract

Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 μM of CdCl2, 2 μM of Na2SeO4 and a mixture of both CdCl2 and Na2SeO4. The seedlings were cultured till the first leaf reached about 1 cm in length. Cadmium-induced changes in the activity of both photosystems were partly diminished by Se presence in the nutrient medium. Electron microscopy photographs confirmed less degradation in chloroplasts of plants cultured on media containing Se. It is suggested that sucrose groups of starch, which is deposited in greater amounts in Cd-stressed plants, may act as traps for free radicals produced under those conditions.

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Abbreviations

A:

Constant of hyperfine splitting (HFS)

ABS/CS:

Light energy absorbed by leaf cross-section (CS).

DFT:

Density-functional theory

ETo/ABS:

Probability that an absorbed photon will move an electron into the electron transport chain

ETo/CS:

Quantum yield of photosynthetic electron transport chain after QA per CS

ETo/TRo :

Efficiency with which a trapped exciton can move an electron into the electron transport chain

Fm′:

Fluorescence with all PSII reaction centres closed in light-exposed leaves

Fo′:

Fluorescence in leaves previously exposed to light, darkened just before measurement

Fs :

Steady-state fluorescence in light-exposed leaves

Fv′/Fm′:

PS2 antenna trapping efficiency

Fv′:

Variable fluorescence in light-adapted leaves (Fv′ = Fm′ − Fo′)

g :

Values of g tensor

NPQ:

Non-photochemical quenching of chlorophyll a fluorescence

OEC:

Fraction of O2 evolving centres PS2 in comparison with the control sample

PI(CS) :

Performance index, defined on a cross section (CS) basis

PPFD:

Photosynthetic photon flux density

PS2:

Photosystem II

*QA :

The first stable electron acceptor in PS2

qP:

Photochemical quenching of chlorophyll a fluorescence

RC/CS:

Number of active reaction centres (RC) in the state of fully reduced PS2 RC

Sm:

The working integral of the energy needed to close all RCs

Sm/t Fmax :

Expresses the average (av) redox state (QA −/QA) in the time span from 0 to Fmax and therefore, the average fraction of open (op) RC during the time needed to complete the closure (cl) of all the RC; Sm/t Fmax = [RCop/(RCcl + RCop)]av

TRo/ABS:

Maximum quantum yield of primary photochemistry

TRo/CS:

Quantum yield of primary photochemistry (from RC to QA) per CS

ΦPS2 :

Quantum yield of PS2 photochemistry

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Authors and Affiliations

  1. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Krakow, Poland

    Maria Filek

  2. Institute of Biology, Pedagogical University, Podbrzezie 3, 31-054, Krakow, Poland

    Maria Filek

  3. Department of Plant Physiology, Agriculture University, Podłużna 3, 30-239, Krakow, Poland

    Janusz Kościelniak

  4. Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland

    Maria Łabanowska & Ewa Bidzińska

  5. Institute of Cell Biology, M. Copernicus University, Gagarina 6, 87-100, Torun, Poland

    Elżbieta Bednarska

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  1. Maria Filek
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Correspondence to Maria Filek.

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Filek, M., Kościelniak, J., Łabanowska, M. et al. Selenium-induced protection of photosynthesis activity in rape (Brassica napus) seedlings subjected to cadmium stress. Fluorescence and EPR measurements. Photosynth Res 105, 27–37 (2010). https://doi.org/10.1007/s11120-010-9551-y

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