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Bacteriochlorophyll-c Homolog Composition in Green Sulfur Photosynthetic Bacterium Chlorobium vibrioformeDependent on the Concentration of Sodium Sulfide in Liquid Cultures

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Abstract

Green sulfur photosynthetic bacteria Chlorobium (Chl.) vibrioforme (DSM 263 strain and NCIB 8327 substrain possessing BChl-c) and Chl. tepidum (ATCC 49652) were photoautotrophically grown in liquid cultures containing different concentrations of sodium sulfide (Na2S). BChl-c homologs possessing a methyl group at the 12-position tended to increase in cells of the two strains of Chl. vibrioforme cultured under high Na2S concentrations. In contrast, the Na2S concentration in liquid cultures did not affect the relative composition of BChl-c homologs in Chl. tepidum. 8-Propyl-12-methyl([P,M])-BChl-c homolog, which has been little observed in usual cultivations, could be isolated by reverse-phase high-performance liquid chromatography from the cells of Chl. vibrioforme grown under high Na2S contents. The [P,M]-BChl-c homolog has the R-configuration at the 31-position, which was determined by 1H-NMR analyses.

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Abbreviations

BChl:

bacteriochlorophyll

Chl:

chlorophyll

C-substrain:

substrain possessing BChl-c

DSMZ:

Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH

[E,E]:

8-ethyl-12-ethyl

[E,M]:

8-ethyl-12-methyl

FAB:

fast atom bombardment

HPLC:

high performance liquid chromatography

[I,E]:

8-isobutyl-12-ethyl

[I,M]:

8-isobutyl-12-methyl

MS:

mass spectrometry

[P,E]:

8-propyl-12-ethyl

[P,M]:

8-propyl-12-methyl

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, 525-8577, Kusatsu, Shiga, Japan

    Yoshitaka Saga, Shigeaki Osumi, Hirohisa Higuchi & Hitoshi Tamiaki

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  1. Yoshitaka Saga
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  2. Shigeaki Osumi
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  3. Hirohisa Higuchi
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  4. Hitoshi Tamiaki
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Correspondence to Hitoshi Tamiaki.

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Saga, Y., Osumi, S., Higuchi, H. et al. Bacteriochlorophyll-c Homolog Composition in Green Sulfur Photosynthetic Bacterium Chlorobium vibrioformeDependent on the Concentration of Sodium Sulfide in Liquid Cultures. Photosynth Res 86, 123–130 (2005). https://doi.org/10.1007/s11120-005-5301-y

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  • DOI: https://doi.org/10.1007/s11120-005-5301-y

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