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Development of a Core Collection of Six-Rowed Hulless Barley from the Qinghai-Tibetan Plateau

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Abstract

A core collection reflects the diversity of the larger germplasm collection and makes the evaluation of genetic resources more effective. To improve barley germplasm management, we propose the construction of a core genetic collection derived from a set of 204 six-rowed hulless barley accessions (39 registered cultivars and 165 landraces) native to the Qinghai-Tibetan Plateau (QTP). A sampling percentage of 20% was predicted by the maximization strategy. Five different sampling strategies (Mstrat, Random, REMC, SBS, and SFS) were applied to establish 5 primary core collections from the original 204 accessions using 1750 single-nucleotide polymorphism (SNP) markers. After the evaluation and analysis of core collection representativeness, the Mstrat strategy was determined to be a valid method for developing a core collection. A total of 41 barley accessions were obtained, including 11 registered cultivars and 30 landraces. Genetic structure analysis of the 204 barley accessions showed that the core collection was evenly distributed throughout the initial collection, indicating that the core collection represented the larger germplasm well. A subset of 28 SNP markers were produced and used to distinguish all 204 barley accessions from each other. We believe the collection of 28 SNP markers will be incredibly helpful to barley breeders. Furthermore, the core collection ensures the long-term conservation of genetic resources and will support improvements to six-rowed hulless barley from the Qinghai-Tibetan Plateau.

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Funding

This research was financially supported by the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (Grant No. 2019QZKK0303), Construction Project for Innovation Platform of Qinghai province (2017-ZJ-Y14), Science and Technology Service Network Initiative (STS) of CAS, and Innovation Academy for Seed Design, CAS.

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Author notes

  1. Jinqing Xu and Lei Wang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Qinghai Provincial Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China

    Jinqing Xu, Lei Wang, Handong Wang, Chengzhi Mao, Doudou Kong, Huaigang Zhang & Yuhu Shen

  2. Innovation Academy for Seed Design, CAS, Xining, 810008, China

    Jinqing Xu, Lei Wang, Handong Wang, Huaigang Zhang & Yuhu Shen

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Chengzhi Mao & Doudou Kong

  4. Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, 810008, China

    Shengyun Chen

Authors
  1. Jinqing Xu
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  2. Lei Wang
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  3. Handong Wang
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  4. Chengzhi Mao
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  6. Shengyun Chen
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  7. Huaigang Zhang
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  8. Yuhu Shen
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Correspondence to Yuhu Shen.

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Key Message

A core collection of six-rowed naked hulless barley from the Qinghai-Tibetan Plateau (QTP), including 41 barley accessions, was established. A subset of 28 SNP markers were produced and used to distinguish all 204 barley accessions from each other.

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Xu, J., Wang, L., Wang, H. et al. Development of a Core Collection of Six-Rowed Hulless Barley from the Qinghai-Tibetan Plateau. Plant Mol Biol Rep 38, 305–313 (2020). https://doi.org/10.1007/s11105-020-01196-0

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  • DOI: https://doi.org/10.1007/s11105-020-01196-0

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