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A Leucine-Rich Repeat Receptor-like Kinase from the Antarctic Moss Pohlia nutans Confers Salinity and ABA Stress Tolerance

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Abstract

Plant leucine-rich repeats receptor-like kinases (LRR-RLKs) play key roles in plant growth, development, and responses to environmental stresses. However, the functions of LRR-RLKs in bryophytes are still not well documented. Here, a putative LRR-RLK gene, PnLRR-RLK, was cloned and characterized from the Antarctic moss Pohlia nutans. Phylogenetic analysis revealed that PnLRR-RLK protein was clustered with the Arabidopsis thaliana LRR XI family proteins. Subcellular localization analysis of PnLRR-RLK revealed that it was mainly localized on plasma membrane. The expression of PnLRR-RLK was induced by mock high salinity, cold, drought, and exogenously supplied abscisic acid (ABA) and methyl jasmonate (MeJA). Meanwhile, the overexpression of PnLRR-RLK showed an increased tolerance of transgenic Arabidopsis to salt and ABA stresses than that of the wild type (WT) plants. Furthermore, the expression levels of several salt tolerance genes (AtHKT1, AtSOS3, AtP5CS1, and AtADH1) and an ABA negatively regulating gene AtABI1 were significantly increased in transgenic plants. Meanwhile, the expression levels of ABA biosynthesis genes (AtNCED3, AtABA1, and AtAAO3) and ABA early response genes (AtMYB2, AtRD22, AtRD29A, and AtDREB2A) were decreased in transgenic Arabidopsis after salt stress treatment. Therefore, these results suggested that PnLRR-RLK might involve in regulating salt stress-related and ABA-dependent signaling pathway, thereby contribute to the salinity tolerance of the Antarctic moss P. nutans.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (41206176 and 41476174), Basic Scientific Fund for National Public Research Institutes of China (2014T04), Natural Science Foundation of Shandong Province (ZR2014DQ012).

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Authors and Affiliations

  1. National Glycoengineering Research Center and College of Life Science, Shandong University, Jinan, 250100, China

    Jing Wang, Pengying Zhang & Kaoshan Chen

  2. Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061, China

    Shenghao Liu & Bailin Cong

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  1. Jing Wang
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  2. Pengying Zhang
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  3. Shenghao Liu
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Correspondence to Pengying Zhang.

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Fig. S1

Protein domain and sequence alignment. a Schematic depiction of the major domains in PnLRR-RLK. SP signal peptide, LRR leu-rich repeat region, TM transmembrane domain, the kinase domain is also indicated. b Amino acid sequence alignment between PnLRR-RLK and other LRR-RLK family members Physcomitrella patens subsp. Patens (EDQ58290), Arabidopsis thaliana (AED97817), and Medicago sativa (KEH41270). Multiple sequence alignments were conducted using ClustalW. Black boxes show identical amino acid residues, and gray shades show similar residues. Deletions are indicated by dashes to allow maximum alignment. (PDF 1.35 mb)

Fig. S2

Phylogenetic analysis of LRR-RLK. Phylogenetic relationships between PnLRR-RLK and other reported LRR- RLKs from higher plants. (PDF 47 kb)

Fig. S3

a PnLRR-RLK promotes the growth of Arabidopsis seedling after salinity treatment. b Measurement of root length of salinity-stressed Arabidopsis seedlings (a). Vertical bars are presented as means ± SE, and asterisks indicate significant differences of means between the AtOE lines and the WT plants at p < 0.05. Bar = 1 cm (PDF 210 kb)

Fig. S4

a PnLRR-RLK promotes the growth of Arabidopsis seedling after ABA treatment. b Statistical analysis of the root length in transgenic Arabidopsis (a). Vertical bars are means ± SE and asterisks indicate significant differences of means between the AtOE lines and the WT plants at p < 0.05. Bar = 1 cm (PDF 243 kb)

Table S1

Primers for gene clone, plasmid construction, and real-time PCR analysis. (DOC 60 kb)

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Wang, J., Zhang, P., Liu, S. et al. A Leucine-Rich Repeat Receptor-like Kinase from the Antarctic Moss Pohlia nutans Confers Salinity and ABA Stress Tolerance. Plant Mol Biol Rep 34, 1136–1145 (2016). https://doi.org/10.1007/s11105-016-0994-y

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