Abstract
Histidine triad nucleotide binding protein (HINT1) belongs to a histidine triad (HIT) superfamily, which contains a highly conserved His-X-His-X-His-XX motif (X is a hydrophobic amino acid) and plays an important role in many biological processes. In this study, we have isolated the full-length cDNA of an HINT1 homologue from maize (Zea mays L.), designated as Zm-HINT1. The full-length cDNA of Zm-HINT1 consists of 729 bp with an ORF encoding a 138-amino acid protein. The deduced amino acid sequence of Zm-HINT1 shows high sequence homology to the mammalian HINT1 and contains conserved domains including the HIT motif, helical regions and β-strands, which are the characteristics of HINT1 proteins. The phylogenetic analysis has revealed that Zm-HINT1 is branched along with Caenorhabditis elegans HINT1. RT-PCR analysis has revealed that Zm-HINT1 is ubiquitously expressed in maize tissues but not in the pericarp, thus suggesting that Zm-HINT1 may not be related to the production of fibrin. Furthermore, expression levels of Zm-HINT1 have increased rapidly following treatment with salicylic acid. Taken together, these results indicate that Zm-HINT1 is a mammalian HINT1 homologue and may be involved in the immune response of maize.
References
Ahel I, Rass U, El-Khamisy SF, Katyal S, Clements PM, McKinnon PJ, Caldecott KW, West SC (2006) The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates. Nature 443(7112):713–716
Bardaweel S, Pace J, Chou TF, Cody V, Wagner CR (2010) Probing the impact of the echint C-terminal domain on structure and catalysis. J Mol Bio 404:627–638
Bennetzen JL, Hake S (2009) Handbook of maize: genetics and genetics. Springer, New York
Bieganowski P, Garrison PN, Hodawadekar SC, Faye G, Barnes LD, Brenner C (2002) Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. J Biol Chem 227(13):10852–10860
Brenner C, Garrison P, Gilmour J, Peisach D, Ringe D, Petsko GA, Lowenstein JM (1997) Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins. Nat Struct Biol 4(3):231–238
Carzoli FG, Michelotti V, Fambrini M, Salvini M, Pugliesi C (2009) Molecular cloning and organ-specific expression of two gibberellin 20-oxidase genes of Helianthus annuus. Plant Mol Biol Rep 27:144–152
Chou TF, Sham YY, Wagner CR (2007) Impact of the C-terminal loop of histidine triad nucleotide binding protein (Hint1) on substrate specificity. Biochemistry 46:13074–13079
Date H, Onodera O, Tanaka H, Iwabuchi K, Uekawa K, Igarashi S, Koike R, Hiroi T, Yuasa T, Awaya Y, Sakai T, Takahashi T, Nagatomo H, Sekijima Y, Kawachi I, Takiyama Y, Nishizawa M, Fukuhara N, Saito K, Sugano S, Tsuji S (2001) Early-onset ataxia with ocular motor apraxia and hypoalbuminemia is caused by mutations in a new HIT superfamily gene. Nat Genet 29(2):184–188
Fritig B, Kauffmann S, Dumas B, Geoffroy P, Kopp M, Legrand M (2007) Mechanism of the hypersensitivity reaction of plants. Wiley, New York, pp 92–108
Gao CQ, Wang YC, Liu GF, Wang C, Jiang J, Yang CP (2010) Cloning of ten peroxidase (POD) genes from Tamarix hispida and characterization of their responses to abiotic stress. Plant Mol Biol Rep 28:77–89
Klein MG, Yao Y, Slosberg ED, Lima CD, Doki Y, Weinstein IB (1998) Characterization of PKCI and comparative studies with FHIT, related members of the HIT protein family. Exp Cell Res 244(1):26–32
Kong FN, Jiang SM, Meng XB, Song CL, Shi JF, Jin DM, Jiang SL, Wang B (2009) Cloning and characterization of the DHDPS gene encoding the lysine biosynthetic enzyme dihydrodipocolinate synthase from Zizania latifolia (Griiseb). Plant Mol Biol Report 27:199–208
Korsisaari N, Makela TP (2000) Interactions of Cdk7 and Kin28 with Hint/PKCI-1 and Hnt1 histidine triad proteins. J Biol Chem 275:34837–34840
Krakowiak A, Pace HC, Blackburn GM, Adams M, Mekhalfia A, Kaczmarek R, Baraniak J, Stec W, Brenner C (2004) Biochemical, crystallographic, and mutagenic characterization of Hint, the AMP-lysine hydrolase, with novel substrates and inhibitors. J Biol Chem 279(18):18711–18716
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
Li H, Zhang Y, Su T, Santella RM, Weinstein IB (2006) Hint1 is a haplo-insufficient tumor suppressor in mice. Oncogene 25(5):713–721
Liu XM, Anderson JM, Pijut PM (2010) Cloning and characterization of Prunus serotina AGAMOUS, a putative flower homeotic gene. Plant Mol Biol Rep 28:193–203
Luo T, Deng WY, Zeng J, Zhang FL (2009) Cloning and characterization of a stearoyl–acyl carrier protein desaturase gene from Cinnamomum longepaniculatum. Plant Mol Biol Rep 27:13–19
Moreira MC, Barbot C, Tachi N, Kozuka N, Uchida E, Gibson T, Mendonça P, Costa M, Barros J, Yanagisawa T, Watanabe M, Ikeda Y, Aoki M, Nagata T, Coutinho P, Sequeiros J, Koenig M (2001) The gene mutated in ataxia-ocular apraxia 1 encodes the new HIT/Zn-finger protein aprataxin. Nat Genet 29(2):189–193
Mozier NM, Walsh MP, Pearson JD (1991) Characterization of a novel zinc binding site of protein kinase C inhibitor-1. FEBS Lett 279(1):14–18
Razin E, Zhang ZC, Nechushtan H, Frenkel S, Lee YN, Arudchandran R, Rivera J (1999) Suppression of microphthalmia transcriptional activity by its association with protein kinase C-interacting protein 1 in mast cells. J Biol Chem 274:34272–34276
Ryals J, Lawton KA, Delaney TP, Friedrich L, Kessmann H, Neuenschwander U, Uknes S, Vernooij B, Weymann K (1995) Signal transduction in systemic acquired resistance. Proc Natl Acad Sci USA 92(10):4202–4205
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425
Schultz J, Milpetz F, Bork P, Ponting CP (1998) SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci USA 95:5857–5864
Su T, Suzui M, Wang L, Lin CS, Xing WQ, Weinstein IB (2003) Deletion of histidine triad nucleotide-binding protein 1/PKC-interacting protein in mice enhances cell growth and carcinogenesis. Proc Natl Acad Sci USA 100(13):7824–7829
Vyroubalová S, Václavíková K, Turecková V, Novák O, šmehilová M, Hluska T, Ohnoutková L, Frébort I, Galuszka P (2009) Characterization of new maize genes putatively involved in cytokinin metabolism and their expression during osmotic stress in relation to cytokinin levels. Plant Physiology 151:433–447
Wagner CR, Iyer VV, Mclntee EJ (2000) Pronucleotides: toward the in vivo delivery of antiviral and anticancer nucleotides. Med Res Rev 20:417–451
Wu CX, Gao XH, Kong XQ, Zhao YX, Zhang H (2009) Molecular cloning and functional analysis of a Na+/H+ antiporter gene ThNHXI from a halophytic plant Thellungiella halophila. Plant Mol Biol Rep 27:1–12
Wu LJ, Wu XZ, Deng HK, Huang YQ (2010) First identification and functional analysis of a histidine triad nucleotide binding protein in an invertebrate species Haliotis diversicolor supertexta. Dev Comp Immunol 34:76–83
Zhuang YL, Ren GJ, He CM, Li XY, Meng QM, Zhu CF, Wang RC, Zhang J (2010) Cloning and characterization of a maize cDNA encoding glutamate decarboxylase. Plant Mol Biol Rep 28:620–626
Acknowledgements
We thank Dr. Haifeng Pan for his helpful suggestions. This work was supported by the National Basic Research Program of China (No. 2009CB118400), Science and Technology Development Programs of Henan Province (102102110171), and China Postdoctoral Science Foundation (20100470993).
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Wu, L., Wang, X., Wu, L. et al. Molecular cloning and expression analysis of an HINT1 homologue from maize (Zea mays L.). Plant Mol Biol Rep 29, 1006–1012 (2011). https://doi.org/10.1007/s11105-011-0296-3
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DOI: https://doi.org/10.1007/s11105-011-0296-3