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Molecular cloning and expression analysis of an HINT1 homologue from maize (Zea mays L.)

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Abstract

Histidine triad nucleotide binding protein (HINT1) belongs to a histidine triad (HIT) superfamily, which contains a highly conserved His-X-His-X-His-XX motif (X is a hydrophobic amino acid) and plays an important role in many biological processes. In this study, we have isolated the full-length cDNA of an HINT1 homologue from maize (Zea mays L.), designated as Zm-HINT1. The full-length cDNA of Zm-HINT1 consists of 729 bp with an ORF encoding a 138-amino acid protein. The deduced amino acid sequence of Zm-HINT1 shows high sequence homology to the mammalian HINT1 and contains conserved domains including the HIT motif, helical regions and β-strands, which are the characteristics of HINT1 proteins. The phylogenetic analysis has revealed that Zm-HINT1 is branched along with Caenorhabditis elegans HINT1. RT-PCR analysis has revealed that Zm-HINT1 is ubiquitously expressed in maize tissues but not in the pericarp, thus suggesting that Zm-HINT1 may not be related to the production of fibrin. Furthermore, expression levels of Zm-HINT1 have increased rapidly following treatment with salicylic acid. Taken together, these results indicate that Zm-HINT1 is a mammalian HINT1 homologue and may be involved in the immune response of maize.

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Acknowledgements

We thank Dr. Haifeng Pan for his helpful suggestions. This work was supported by the National Basic Research Program of China (No. 2009CB118400), Science and Technology Development Programs of Henan Province (102102110171), and China Postdoctoral Science Foundation (20100470993).

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Correspondence to Yanhui Chen.

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Wu, L., Wang, X., Wu, L. et al. Molecular cloning and expression analysis of an HINT1 homologue from maize (Zea mays L.). Plant Mol Biol Rep 29, 1006–1012 (2011). https://doi.org/10.1007/s11105-011-0296-3

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