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Ectopic Expression of the Tomato Mi-1 Gene Confers Resistance to Root Knot Nematodes in Lettuce (Lactuca sativa)

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Abstract

The full genomic region of the root knot nematode (Meloidogyne spp.) resistance gene Mi-1 was cloned from tomato and transformed into lettuce to investigate its function in a heterologous system. Transgenic lettuce lines containing the Mi-1 gene were developed using Agrobacterium-mediated transformation. Ectopic expression of the Mi-1 gene was observed in transgenic lines, and resistance to root knot nematode was improved.

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Abbreviations

MS:

Murashige and Skoog medium

BA:

6-Benzyladenine

NAA:

α-Naphthaleneacetic acid

SDS:

Sodium dodecyl sulfate

NPTII :

Neomycin phosphotransferase gene

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription-polymerase chain reaction

FAA:

Formalin–acetic acid–alcohol

UTR:

Untranslated region

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Acknowledgments

The authors thank Dr Zhangjun Fei (Boyce Thompson Institute for Plant Research, Cornell University) for critical review of this manuscript. This work was supported by grants from Major State Basic Research Development Program (2009CB119000), National Nature Science Foundation of China (no. 30800756 and no. 30671417), and Xinjiang Corps Doctor Fund of China (no. 2008JC04).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Li-Ying Zhang, Yu-Yang Zhang, Ru-Gang Chen, Jun-Hong Zhang, Tao-Tao Wang, Han-Xia Li & Zhi-Biao Ye

  2. National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing, 100089, China

    Li-Ying Zhang

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  1. Li-Ying Zhang
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  2. Yu-Yang Zhang
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  3. Ru-Gang Chen
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  4. Jun-Hong Zhang
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  5. Tao-Tao Wang
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  6. Han-Xia Li
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  7. Zhi-Biao Ye
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Corresponding author

Correspondence to Zhi-Biao Ye.

Additional information

L.-Y. Zhang and Y.-Y. Zhang contributed equally to the work.

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Zhang, LY., Zhang, YY., Chen, RG. et al. Ectopic Expression of the Tomato Mi-1 Gene Confers Resistance to Root Knot Nematodes in Lettuce (Lactuca sativa). Plant Mol Biol Rep 28, 204–211 (2010). https://doi.org/10.1007/s11105-009-0143-y

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