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Isolation and Analysis of Rice Rf1-Orthologus PPR Genes Co-segregating with Rf3 in Maize

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Abstract

Using an in silico cloning approach, five putative maize pentatricopeptide repeat (PPR)-containing protein genes (PPR-814a, PPR-814b, PPR-814c, PPR-816, PPR-817) with complete open reading frames were identified in the inbred line S-Mo17Rf3Rf3. The amino acid sequence indicated that these genes encoded mitochondrially targeted proteins containing repeats of a 35-aa PPR motif. The genes were mapped into the interval umc1525–bnlg1520 on chromosome 2. In a non-restoring genotype, we identified three homologous genes that contained deletions or nucleotide substitutions in the coding region. Sequence analysis revealed that one of the three genes (PPR-814a, PPR-814b, PPR-814c) could be considered a candidate restorer gene for S male sterility cytoplasm, and linkage analysis demonstrated that the genes co-segregated with the fertility restorer gene Rf3.

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Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, Beijing, 100193, China

    Xiang-Bo Xu, Zhan-Xian Liu, Deng-Feng Zhang, Ying Liu, Wei-Bin Song, Jian-Sheng Li & Jing-Rui Dai

  2. Institute for Application of Atomic Energy, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Xiang-Bo Xu

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  1. Xiang-Bo Xu
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  2. Zhan-Xian Liu
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  3. Deng-Feng Zhang
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  4. Ying Liu
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  5. Wei-Bin Song
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  7. Jing-Rui Dai
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Correspondence to Jing-Rui Dai.

Additional information

This work was supported by Key Project of Chinese National Programs for Fundamental Research and Development (2007CB109006).

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Xu, XB., Liu, ZX., Zhang, DF. et al. Isolation and Analysis of Rice Rf1-Orthologus PPR Genes Co-segregating with Rf3 in Maize. Plant Mol Biol Rep 27, 511–517 (2009). https://doi.org/10.1007/s11105-009-0105-4

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