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Effects of elevated ozone on the contribution of nitrogen rhizodeposition by spring wheat to different soil N pools

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Abstract

Background and aims

Elevated ozone (O3) decreases nitrogen derived from rhizodeposition (NdfR). However, the changes in the partitioning of NdfR in soil N pools due to O3 remain unclear. The aims of this study were to investigate the contribution of NdfR to different soil N pools and its response to elevated O3 conditions.

Methods

Spring wheat was labeled with 15N–urea using a split-root technique under ambient (~40 ppb) and elevated O3 treatments (60 ± 5 ppb and 110 ± 5 ppb) in open-top chambers. Mineral-N, microbial biomass (MB)-N and fixed ammonium (FA)-N in rhizospheric soils were analyzed.

Results

N rhizodeposition contributed 12–33% of mineral N, 10–14% of FA-N and 6–16% of MB-N under ambient O3. Elevated O3 significantly decreased mineral-15NdfR and increased FA-15NdfR but had no significant influence on MB-15NdfR. The decrease in mineral-NdfR was likely due to the decrease in rhizodeposition inputs and the increase in FA-NdfR.

Conclusions

Our results showed that elevated O3 altered the contribution of NdfR to soil N pools. The present study increases our understanding of the dynamics of NdfR and the changes in soil N cycling induced by projected future O3 levels.

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Abbreviations

O3 :

Ozone

N:

Nitrogen

NdfR:

Nitrogen derived from rhizodeposition

NH4 + :

Ammonium

NO3 − :

Nitrate

MB-N:

Microbial biomass nitrogen

FA-N:

Fixed ammonium nitrogen

CK:

Control

LO:

Lower ozone concentration-elevated level

HO:

Higher ozone concentration-elevated level

LC:

Labeling compartment

TC:

Transfer compartment

rootsT :

Roots sampled from the transfer compartment

soilT :

Soil in the transfer compartment

DAF15, DAF30 and DAF45:

15, 30 and 45 days after ozone fumigation

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant number 41730855; 41703005; 41671290) and the National Key Research and Development Program of China (grant number 2016YFD0800103). We appreciate the anonymous reviewers for insightful comments on an earlier version of our manuscript.

Author information

Authors and Affiliations

  1. Institute of Surface-Earth System Science, Tianjin University, Tianjin, 300072, China

    Yanhong Cao, Xinchao Sun & Xueyan Liu

  2. Key Laboratory of Pollution Ecology and Environment Engineering, Institute of Applied Ecology, Chinese Academy of Science, Wenhua Road 72, Shenhe District, Shenyang, 110016, China

    Yi Shi & Caiyan Lu

Authors
  1. Yanhong Cao
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  2. Yi Shi
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  3. Xinchao Sun
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  4. Caiyan Lu
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Corresponding authors

Correspondence to Caiyan Lu or Xueyan Liu.

Additional information

Responsible Editor: Elizabeth M Baggs.

Highlights

• The contribution of N rhizodeposition to soil mineral, microbial biomass and fixed ammonium N pools were investigated.

• Elevated O3 significantly decreased mineral-15NdfR but increased fixed ammonium-15NdfR.

• Elevated O3 had no significant influence on 15NdfR in microbial biomass.

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Cao, Y., Shi, Y., Sun, X. et al. Effects of elevated ozone on the contribution of nitrogen rhizodeposition by spring wheat to different soil N pools. Plant Soil 425, 321–333 (2018). https://doi.org/10.1007/s11104-018-3592-y

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  • DOI: https://doi.org/10.1007/s11104-018-3592-y

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