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Illumina sequencing reveals a rhizosphere bacterial community associated with foxtail millet smut disease suppression

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Abstract

Background and aims

Smut disease is a severe limiting factor in foxtail millet production, caused by the Ustilago crameri fungus. The aim of this study is to characterize specific bacterial populations that might contribute to smut disease suppression.

Methods

Rhizosphere bacterial community compositions of three foxtail millet cultivars differing in smut disease resistance were compared using high throughput Illumina sequencing.

Results

As a disease-resistant cultivar, Jigu20 showed the lowest disease incidence (0.7 %) after Ustilago crameri inoculation, compared with 14.8 % and 62.5 % for Jingu21 and Changnong35, respectively. Under the Ustilago crameri-inoculation condition, higher bacterial diversity was present in the rhizosphere of Jigu20 (I.Ji20) than in other two soil samples (I.Jin21 and I.Chang35). The same trend was observed in enzyme activities of phosphatase, catalase, and polyphenol oxidase. Compared to I.Jin21 and I.Chang35, I.Ji20 harbored higher abundance of Actinobacteria, while lower abundance of Bacteroidetes and Firmicutes. At the genus level, the hierarchical cluster of I.Ji20 was clearly separated from I.Jin21 and I.Chang35. Pearson’s correlation analysis showed that the abundance of Bradyrhizobium and Streptomyces was negatively correlated with smut disease incidence (P < 0.05).

Conclusions

Different smut resistant cultivars occupied different rhizosphere bacterial communities, and smut disease suppression might be correlated with high bacterial diversity.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30470318, 30870454, 31371868, 31500504), National Special Fund for the Construction of Modern Agricultural Technology System (CARS-07-12.5-A10), Research Fund for the Doctoral Program of Higher Education of China (20070108007, 20121401110007) and Shanxi Scholarship Council of China (2009022, 2012013). We are very grateful to Shanghai Personal Biotechnology Co., Ltd for Illumina sequencing.

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Authors and Affiliations

  1. School of Life Science, Shanxi University, Taiyuan, 030006, China

    Yansha Han, Lixia Xu, Liqing Liu, Min Yi & Huilan Yi

  2. Millet Research Institute, Shanxi Academy of Agricultural Science, Changzhi, 046011, China

    Erhu Guo & Aiying Zhang

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  1. Yansha Han
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  2. Lixia Xu
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  3. Liqing Liu
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  4. Min Yi
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  5. Erhu Guo
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  6. Aiying Zhang
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  7. Huilan Yi
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Correspondence to Huilan Yi.

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Han, Y., Xu, L., Liu, L. et al. Illumina sequencing reveals a rhizosphere bacterial community associated with foxtail millet smut disease suppression. Plant Soil 410, 411–421 (2017). https://doi.org/10.1007/s11104-016-3042-7

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