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N- and P-mediated seminal root elongation response in rice seedlings

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Abstract

Aims

In rice, seminal root elongation plays an important role in acquisition of nutrients such as N and P, but the extent to which different N forms and P concentrations affect root growth is poorly understood. This study aimed to examine N- and P-mediated seminal root elongation response and to identify putative QTLs associated with seminal root elongation.

Methods

Seminal root elongation was evaluated in 15 diverse wild and cultivated accessions of rice, along with 48 chromosome segment substitution lines (CSSLs) derived from a cross between the rice variety ‘Curinga’ and Oryza rufipogon (IRGC 105491). Root elongation in response to different forms of N (NH4 +, NO3 − and NH4NO3) and concentrations of P was evaluated under hydroponic conditions, and associated putative QTL regions were identified.

Results

The CSSL parents had contrasting root responses to N and P. Root elongation in O. rufipogon was insensitive to N source and concentration, whereas Curinga was responsive. In contrast to N, seminal root elongation and P concentration was positively correlated. Three putative QTLs for seminal root elongation in response to N were detected on chromosome 1, and one QTL on chromosome 3 was associated with low P concentration.

Conclusions

Genetic variation in seminal root elongation and plasticity of nutrient response may be appropriate targets for marker-assisted selection to improve rice nutrient acquisition efficiency.

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Abbreviations

CSSL:

Chromosome segment substitution line

N:

Nitrogen

NAE:

Nitrogen acquisition efficiency

P:

Phosphorus

PNN:

Partial nitrate nutrition

PAE:

Phosphorus acquisition efficiency

QTL:

Quantitative trait locus

SNP:

Single nucleotide polymorphism

SSR:

Simple sequence repeat

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Acknowledgments

We thank Dr. Edgar Torres (CIAT, Colombia) and Dr. Uga Y (National Institute of Agrobiological Sciences, Japan) for providing the seed materials used this study, and Dr. Joe Tohme, CIAT Agrobiodiversity Research Area Director, for his continuous support. We also thank Dr. T. Ramasubramanian (Sugarcane Breeding Institute, Indian Council of Agricultural Research, Tamil Nadu, India) for his critical evaluation of the manuscript, and are grateful for the assistance of Lucia Chavez and Milton Valencia.

This work was supported by Ministry of foreign Affairs of Japan.

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Authors and Affiliations

  1. International Center for Tropical Agriculture (CIAT), A.A. 6713, Cali, Colombia

    Satoshi Ogawa, Michael Gomez Selvaraj, Angela Joseph Fernando, Mathias Lorieux & Manabu Ishitani

  2. Department of Global Agriculture Science, Graduate School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Satoshi Ogawa

  3. Institut de Recherche pour le Développement (IRD), DIADE Research Unit, Montpellier, France

    Mathias Lorieux

  4. Department of Plant Breeding and Genetics, Cornell University, 240 Emerson Hall, Ithaca, NY, 14853, USA

    Susan McCouch & Juan David Arbelaez

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  1. Satoshi Ogawa
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  2. Michael Gomez Selvaraj
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  3. Angela Joseph Fernando
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  4. Mathias Lorieux
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Correspondence to Michael Gomez Selvaraj.

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Ogawa, S., Selvaraj, M.G., Fernando, A.J. et al. N- and P-mediated seminal root elongation response in rice seedlings. Plant Soil 375, 303–315 (2014). https://doi.org/10.1007/s11104-013-1955-y

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