Abstract
Mesorhizobium loti is a Gram negative bacterium that induces N2-fixing root nodules on the model legume Lotus japonicus. Proteomic analysis in M. loti indicated that 3-phosphoglycerate dehydrogenase (EC. 1.1.1.95, PHGDH) protein content was 2.2 times higher in bacteroids than in cultured bacteria. A M. loti mutant (STM5) with a transposon insertion in the PHGDH gene, mll3875, showed an absolute dependence on serine or glycine in minimal medium for growth. When L. japonicus plants were infected with STM5, the roots formed nodules in numbers comparable to those formed by wild type M. loti; however, the nodules showed very low acetylene reduction activity, and significant starch granule accumulation was observed in the uninfected cells. In such nodules, vast necrosis occurred in the central tissue of the nodules, although bacteroids were detected in the infected cell of the nodules. These data indicate that serine or glycine biosynthesis by PHGDH is important for maintaining symbiosis and nitrogen fixation in L. japonicus nodules.
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Abbreviations
- ARA:
-
Acetylene reduction activity
- dpi:
-
Days post inoculation
- PHGDH:
-
3-Phosphoglycerate dehydrogenase
- STM:
-
Signature-tagged mutagenesis
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Acknowledgments
We thank Mr. Peter Lutes for critical reading of the manuscript. This work was supported by a Grant-in-Aid (19580067) and by the Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Thapanapongworakul, N., Nomura, M., Van Dao, T. et al. 3-Phosphoglycerate dehydrogenase in Mesorhizobioum loti is essential for maintaining symbiotic nitrogen fixation of Lotus japonicus root nodules. Plant Soil 336, 233–240 (2010). https://doi.org/10.1007/s11104-010-0471-6
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DOI: https://doi.org/10.1007/s11104-010-0471-6