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Physiological behaviour, oxidative damage and antioxidative protection of olive trees grown under different irrigation regimes

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Abstract

Irrigation effects were investigated on an 8-year-old olive (Olea europaea L., cv. Cobrançosa) commercial orchard located in northeast Portugal. Trees were subjected to a rainfed control (T0) and three treatments (T1, T2, T3) that received a seasonal water amount equivalent to 30%, 60% and 100% of the estimated local evaporative demand by a drip irrigation system. Irrigation increases the photosynthetic activity of olive trees, in association with increases in water status, and reduces the midday and afternoon depression in gas exchange. The closely association between photosynthetic rate (A) and stomatal conductance (g s) revealed that the decline in net photosynthesis over the course of the day was largely a consequence of stomatal limitation. However, the ratio of intercellular to atmospheric CO2 concentration increased markedly from morning to midday in non-irrigated plants, in spite of lower g s, suggesting that non-stomatal limitations of photosynthesis also occur when environmental conditions become more stressful. The occurrence of perturbations at chloroplastic level in rainfed plants was demonstrated by a lower maximum photochemical efficiency of photosystem II during the afternoon. Chlorophyll fluorescence measurements also revealed the occurrence of a dynamic photoinhibition in irrigated trees, mainly in T2 and T3, which seemed to be effective in protecting the photosynthetic apparatus from photodamage. Irrigation enhances antioxidant protection and decreases the oxidative damage at leaf level. Leaves grown under rainfed conditions revealed symptoms of oxidative stress, like the reduction (14%) in chlorophyll concentration and the increased levels (57%) of lipid peroxidation. We also found that the scavenging function of superoxide dismutase was impaired in rainfed plants. In contrast, the low thiobarbituric acid reactive substances concentration in T3 indicates that irrigation enhances the repairing mechanisms and decreases the oxidative damage by lipid peroxidation. Accordingly, leaves in T3 treatment had high levels of –SH compounds and the highest antioxidant potential. Meanwhile, the finding that guaiacol peroxidase activity increased in rainfed plants, associated with the appearance of oxidative damage, suggests that this enzyme has no major antioxidative function in olive.

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References

  • Abd-El-Rahman AA, Shalaby AF, Balegh M (1966) Water economy of olive under desert conditions. Flora 156:202–219

    Google Scholar 

  • Ali MB, Hahn E-J, Paek K-Y (2005) Effects of temperature on oxidative stress defense systems, lipid peroxidation and lipoxygenase activity in Phalaenopsis. Plant Physiol Biochem 43:213–223

    Article  PubMed  CAS  Google Scholar 

  • Amundson RG, Kohut RJ, Laurence JA, Fellows S, Colavito LJ (1993) Moderate water stress alters carbohydrate content and cold tolerance of red spruce foliage. Environ Exp Bot 33:390–393

    Article  Google Scholar 

  • Angelopoulos K, Dichio B, Xiloyannis C (1996) Inhibition of photosynthesis in olive trees (Olea europaea L.) during water stress and rewatering. J Exp Bot 47:1093–1100

    Article  CAS  Google Scholar 

  • Asada K (1992) Ascorbate peroxidase—a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant 85:235–241

    Article  CAS  Google Scholar 

  • Asada K (1999) The water–water cycle in chloroplasts scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50:601–639

    Article  PubMed  CAS  Google Scholar 

  • Bacelar EA, Correia CM, Moutinho-Pereira JM, Gonçalves BC, Lopes JI, Torres-Pereira JM (2004) Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions. Tree Physiol 24:233–239

    PubMed  Google Scholar 

  • Bacelar EA, Santos DL, Moutinho-Pereira JM, Gonçalves BC, Ferreira HF, Correia CM (2006) Immediate responses and adaptative strategies of three olive cultivars under contrasting water availability regimes: changes on structure and chemical composition of foliage and oxidative damage. Plant Sci 70:596–605

    Article  CAS  Google Scholar 

  • Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal Bioch 72:248–254

    Article  CAS  Google Scholar 

  • Bussis D, Kauder F, Heineke D (1998) Acclimation of potato plants to polyethylene glycol-induced water deficit. I. Photosynthesis and metabolism. J Exp Bot 49:1349–1360

    Article  CAS  Google Scholar 

  • Caturla N, Pérez-Fons L, Estepa A, Micol V (2005) Differential effects of oleuropein, a biophenol from Olea europaea, on anionic and zwiterionic phospholipid model membranes. Chem Phys Lipids 137:2–17

    Article  PubMed  CAS  Google Scholar 

  • Chaves MM, Pereira JS, Maroco J (2003) Understanding plant response to drought – from genes to the whole plant. Funct Plant Biol 30:1–26

    Article  Google Scholar 

  • Costa H, Gallego SM, Tomaro ML (2002) Effect of UV-B radiation on antioxidant defense system in sunflower cotyledons. Plant Sci 162:939–945

    Article  CAS  Google Scholar 

  • Egert M, Tevini M (2002) Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environ Exp Bot 48:43–49

    Article  CAS  Google Scholar 

  • Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77

    Article  PubMed  CAS  Google Scholar 

  • Evans JR (1993) Photosynthetic acclimation and nitrogen partitioning within a lucerne canopy. I Canopy characteristics. Aust J Plant Physiol 20:55–67

    CAS  Google Scholar 

  • Faria T, García-Plazaola JI, Abadía A, Cerasoli S, Pereira JS, Chaves MM (1996) Diurnal changes in photoprotective mechanisms in leaves of cork oak (Quercus suber L.) during summer. Tree Physiol 16:115– 123

    PubMed  Google Scholar 

  • Faria T, Silvério D, Breia E, Cabral R, Cabral A, Abadia A, Abadia J, Pereira JS, Chaves MM (1998) Differences in response of carbon assimilation to summer stress (water deficits, high light and temperature) in four Mediterranean trees species. Physiol. Plant 102:419–428

    Article  CAS  Google Scholar 

  • Fernández JE, Moreno F (1999) Water use by the olive tree. In: Kirkham MB (ed) Water use in crop production. The Haworth Press, Binghamton, New York, pp 101–162

    Google Scholar 

  • Flexas J, Escalona JM, Medrano H (1998) Down-regulation of photosynthesis by drought under field conditions in grapevines leaves. Aust J Plant Physiol 25:893–900

    Google Scholar 

  • Flohé L, Otting F (1984) Superoxide dismutase assays. Method Enzymol 105:93–104

    Google Scholar 

  • Giorgieva K, Yordanov I (1993) Temperature dependence of chlorophyll fluorescence parameters of pea seedlings. J Plant Physiol 142:151–155

    Google Scholar 

  • Giorio P, Sorrentino G, d’Andria R (1999) Stomatal behaviour, leaf water status and photosynthetic response in field-grown olive trees under water deficit. Environ Exp Bot 42:95–104

    Article  Google Scholar 

  • Halliwell B, Gutteridge JMC, Cross CE (1992) Free radicals, antioxidants and human disease: where are we now? J Lab Clin Med 119:598–620

    PubMed  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  PubMed  CAS  Google Scholar 

  • Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol. Biol 47:377–403

    Article  PubMed  CAS  Google Scholar 

  • INMG (1991) O clima de Portugal. Normais climatológicas da região de “Trás-os-Montes e Alto Douro e Beira Interior”, correspondentes a 1951–1980. Fascículo XLIX, Vol. 3 – 3ª Região. INMG, Lisboa

  • Irigoyen JJ, Emerich DW, Sánchez-Díaz M (1992) Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60

    Article  CAS  Google Scholar 

  • Jorba J, Tapia L, Sant D (1985) Photosynthesis, leaf water potential, and stomatal conductance in Olea europaea under wet and drought conditions. Acta Hortic 171:237–246

    Google Scholar 

  • Kitts DD, Wijewickreme AN, Hu C (2000) Antioxidant properties of North American ginseng extracts. Mol Cell Biochem 203:1–10

    Article  PubMed  CAS  Google Scholar 

  • Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Plant Physiol 42:313– 349

    CAS  Google Scholar 

  • Kronfuβ G, Polle A, Tausz M, Havranek W, Wieser G (1998) Effects of ozone and mild drought stress on gas exchange, antioxidants and chloroplast pigments in current-year needles of young Norway spruce (Picea abies L., Karst.). Trees 12:482–489

    Google Scholar 

  • Kurup SS, Nalwadi UG, Basarkar PW, Geibel M, Treutter D (1994) Phenolic biosynthesis in relation to moisture stress in marigold (Tagetes erecta L.). Acta Hort 381:488–493

    CAS  Google Scholar 

  • Laloue H, Weber-Lotfi F, Lucau-Danila A, Guillemaut P (1997) Identification of ascorbate and guaiacol peroxidases in needle chloroplasts of spruce trees. Plant Physiol Biochem 35:341–346

    CAS  Google Scholar 

  • Liang Y, Hu F, Maocheng Y, Yu J (2003) Antioxidative defenses and water deficit induced oxidative damage in rice (Oryza sativa L.) growing on non-flooded paddy soils with ground mulching. Plant Soil 257:407–416

    Article  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Lima AL, DaMatta FM, Pinheiro HA, Totola MR, Loureiro ME (2002) Photochemical responses and oxidative stress in two clones of Coffea canephora under water deficit conditions. Environ Exp Bot 47:239–247

    Article  CAS  Google Scholar 

  • Marron N, Delay D, Petit J-M, Dreyer E, Kahlem G, Delmotte FM, Brignolas F (2002) Physiological traits of two Populus × euramericana clones, Luisa Avanzo and Dorskamp, during a water stress and re-watering cycle. Tree Physiol 22:849–858

    PubMed  CAS  Google Scholar 

  • Millard P (1988) The accumulation and storage of nitrogen by herbaceous plants. Plant Cell Environ 11:1–8

    Article  CAS  Google Scholar 

  • Mirecki R, Teramura AH (1984) Effects of ultraviolet-B irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiol 74:475–480

    Article  PubMed  CAS  Google Scholar 

  • Møller IM (2001) Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annu Rev Plant Physiol Plant Mol Biol 52:561–591

    Article  PubMed  Google Scholar 

  • Natali S, Bignami C, Fusari A (1991) Water consumption, photosynthesis, transpiration and leaf water potential in Olea europaea L. cv. «Frantoio», at different levels of available water. Agric Med 121:205–212

    Google Scholar 

  • Nogués S, Baker NR (2000) Effects of drought on photosynthesis in Mediterranean plants grown under enhanced UV-B radiation. J Exp Bot 51:1309–1317

    Article  PubMed  Google Scholar 

  • Ogaya R, Peñuelas J (2003) Comparative seasonal gas exchange and chlorophyll fluorescence of two dominant woody species in a Holm Oak forest. Flora 198:132–141

    Google Scholar 

  • Osaki M, Shinano T, Tadano T (1991) Redistribution of carbon and nitrogen compounds from the shoot to the harvesting organs during maturation in field crops. Soil Sci Plant Nutr 37:117–128

    CAS  Google Scholar 

  • Osmond CB (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker NR, Bowyer JR (eds) Photoinhibition of photosynthesis: from molecular mechanisms to the field. BIOS Scientific Publishers Ltd, Oxford, pp 1–24

    Google Scholar 

  • Osório ML, Breia E, Rodrigues A, Osório J, Le Rouxc X, Daudetd FA, Ferreira I, Chaves MM (2006) Limitations to carbon assimilation by mild drought in nectarine trees growing under field conditions. Environ Exp Bot 55:235–247

    Article  CAS  Google Scholar 

  • Passioura JB (1982) Water in the soil–plant–atmosphere continuum. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Physiological plant ecology II. Encyclopedia of plant physiology, vol 12B. Springer- Verlag, Berlin, pp 5–33

    Google Scholar 

  • Santos CV, Caldeira G (1999) Comparative responses of Helianthus annuus plants and calli exposed to NaCl. I. Growth rate and osmotic adjustment in intact plants and calli. J. Plant Physiol 155:769–777

    Google Scholar 

  • Scholander PF, Hammel HT, Bradstreet ED, Hemmingsen EA (1965) Sap pressure in vascular plants. Science 148:339–346

    Article  PubMed  Google Scholar 

  • Schwanz P, Picon C, Vivin P, Dreyer E, Guehl JM, Polle A (1996) Responses of antioxidative systems to drought stress in pedunculate oak and maritime pine as modulated by elevated CO2. Plant Physiol 110:393–402

    PubMed  CAS  Google Scholar 

  • Sesták Z, Castky J, Jarvis PG (1971) Plant photosynthetic production. Manual of methods. Dr. W. Junk Publishers, The Hagge, 818 pp

  • Shalata A, Tal M (1998) The effects of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol Plant 104:169–174

    Article  CAS  Google Scholar 

  • Singh N, Rajini PS (2004) Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85:611–616

    Article  CAS  Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  • Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58

    Article  CAS  Google Scholar 

  • Sofo A, Dichio B, Xiloyannis C, Masia A (2004) Effects of different irradiance levels on some antioxidant enzymes and on malondialdehyde content during rewatering in olive tree. Plant Sci 166:293–302

    Article  CAS  Google Scholar 

  • Souza RP, Machado EC, Silva JAB, Lagôa AMMA, Silveira JAG (2004) Photosynthetic gas exchange, chlorophyll fluorescence and some associated metabolic changes in cowpea (Vigna unguiculata) during water stress and recovery. Environ Exp Bot 51:45–56

    Article  CAS  Google Scholar 

  • Tognetti R, d’Andria R, Morelli G, Calandrelli D, Fragnito F (2004) Irrigation effects on daily and seasonal variations of trunk sap flow and leaf water relations in olive trees. Plant Soil 273:139–155

    Article  CAS  Google Scholar 

  • Turner NC, Long MJ (1980) Errors arising from rapid water loss in the measurement of leaf water potential by the pressure chamber technique. Aust J Plant Physiol 7:527–537

    Article  Google Scholar 

  • Van Breusegem F, Van Montagu M, Inze D (1998) Engineering stress tolerance in maize. Outlook Agr 27:115–124

    Google Scholar 

  • Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agr Food Chem 46:4113–4117

    Article  CAS  Google Scholar 

  • von Caemmerer S, Farquhar GD (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153:376–387

    Article  Google Scholar 

  • Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M (2002) Free radical scavenging properties of wheat extracts. J Agr Food Chem 50:1619–1624

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Financial support by AGRO-INIA program (No. 175) of Portuguese Ministry of Agriculture is gratefully acknowledged.

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Authors and Affiliations

  1. Centre for Technological, Environmental and Life Studies (CETAV), Department of Biological and Environmental Engineering, University of Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal

    Eunice A. Bacelar, Dario L. Santos, José M. Moutinho-Pereira, Berta C. Gonçalves & Carlos M. Correia

  2. Regional Direction of Agriculture of Trás-os-Montes, Quinta do Valongo, 5370-347, Mirandela, Portugal

    João I. Lopes

  3. Division of Climatology, University of Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal

    Timóteo C. Ferreira

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  1. Eunice A. Bacelar
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  2. Dario L. Santos
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  3. José M. Moutinho-Pereira
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Correspondence to Carlos M. Correia.

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Bacelar, E.A., Santos, D.L., Moutinho-Pereira, J.M. et al. Physiological behaviour, oxidative damage and antioxidative protection of olive trees grown under different irrigation regimes. Plant Soil 292, 1–12 (2007). https://doi.org/10.1007/s11104-006-9088-1

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