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Combining a modelling with a genetic approach in establishing associations between genetic and physiological effects in relation to phosphorus uptake

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Abstract

The Pup1 locus confers tolerance to phosphorus (P) deficiency in rice (Oryza sativa L.). Transferring the Pup1 locus to an intolerant genotype increased P uptake by a factor 3 to 4. Lines with the Pup1 locus maintained higher root growth rates under P deficiency, but only as they started to diverge from intolerant lines in P uptake. It was thus not possible to determine if differences in root growth preceded and caused differences in P uptake or whether high root growth was the result of higher external P uptake efficiency (P influx per root size). The purpose of this paper is to review experimental evidence on the effect of Pup1 in light of recent results in modelling cause-and-effect relations between root growth, external efficiency and P uptake. Model simulations suggested that only very small changes in factors enhancing root growth were needed to explain the effect of Pup1 on P uptake. A 22% increase in root fineness or in internal P utilization efficiency (root dry matter per root P) was sufficient to triple P uptake . External root efficiency had to increase by 33 to account for the effect of Pup1. However, the most noticeable effect of increases in external efficiency was a subsequent stimulation of root growth that contributed eight times more to final P uptake compared to the change in external efficiency. Comparisons of model simulations with empirical data suggested that measured differences in external efficiency between Nipponbare and NIL-Pup1 were sufficiently large to account for the increase in P uptake. A segregation analysis using several pairs of contrasting NILs (at the Pup1 locus) further confirmed this as Pup1 co-segregated with external efficiency but not with seedling root growth or internal efficiency.

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  1. International Rice Research Institute, Crop, Soil, and Water Sciences Division, Los Banos, Laguna, The Philippines

    Matthias Wissuwa

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  1. Matthias Wissuwa
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Wissuwa, M. Combining a modelling with a genetic approach in establishing associations between genetic and physiological effects in relation to phosphorus uptake. Plant Soil 269, 57–68 (2005). https://doi.org/10.1007/s11104-004-2026-1

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  • DOI: https://doi.org/10.1007/s11104-004-2026-1

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