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A CONSTANS-like transcriptional activator, OsCOL13, functions as a negative regulator of flowering downstream of OsphyB and upstream of Ehd1 in rice

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Abstract

Flowering time determines the adaptability of crop plants to different local environments, thus being one of the most important agronomic traits targeted in breeding programs. Photoperiod is one of the key factors that control flowering in plant. A number of genes that participate in the photoperiod pathway have been characterized in long-day plants such as Arabidopsis, as well as in short-day plants such as Oryza sativa. Of those, CONSTANS (CO) as a floral integrator promotes flowering in Arabidopsis under long day conditions. In rice, Heading date1 (Hd1), a homologue of CO, functions in an opposite way, which inhibits flowering under long day conditions and induces flowering under short day conditions. Here, we show that another CONSTANS-like (COL) gene, OsCOL13, negatively regulates flowering in rice under both long and short day conditions. Overexpression of OsCOL13 delays flowering regardless of day length. We also demonstrated that OsCOL13 has a constitutive and rhythmic expression pattern, and that OsCOL13 is localized to the nucleus. OsCOL13 displays transcriptional activation activity in the yeast assays and likely forms homodimers in vivo. OsCOL13 suppresses the florigen genes Hd3a and RFT1 by repressing Ehd1, but has no relationship with other known Ehd1 regulators as determined by using mutants or near isogenic lines. In addition, the transcriptional level of OsCOL13 significantly decreased in the osphyb mutant, but remained unchanged in the osphya and osphyc mutants. Thus, we conclude that OsCOL13 functions as a negative regulator downstream of OsphyB and upstream of Ehd1 in the photoperiodic flowering in rice.

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Acknowledgments

We thank Dr. Masahiro Yano (The National Institute of Agrobiological Sciences), Dr. Qifa Zhang (Hua zhong Agricultural University), Dr. Atsushi Yoshimura (Kyushu University) and Gynheung An (Pohang University of Science and Technology) for kindly providing NIL (Hd1, Hd3a and Ghd7), osphya, osphyb, osphyc and oscol4 mutants and their corresponding WTs respectively. This work was supported by the National Nonprofit Institute Research Grant of CAAS-ICS (Grant number: 2015JB01-004) and the National Transgenic Major Program (Grant number: 2015ZX08010004).

Author contributions

Jianmin Wan and Xuanming Liu supervised the project. Jianmin Wan, Xuanming Liu, Peike Sheng and Fuqing Wu designed the research. Peike Sheng wrote the paper, Yiqun Bao and Chuanyin Wu revised the paper. Peike Sheng and Fuqing Wu performed most of the experiments, Junjie Tan, Huan Zhang, Weiwei Ma, Liping Chen and Jiachang Wang performed some of the qRT–PCR analysis, yeast one-hybrid assay and the BiFC assay. Shanshan Zhu, Xiuping Guo and Xin Zhang generated the transgenic plants. Jie Wang, Jiulin Wang and Zhijun Cheng cultivated the transgenic plants in the field.

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Correspondence to Xuanming Liu or Jianmin Wan.

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Peike Sheng and Fuqing Wu have contributed equally to this work.

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Sheng, P., Wu, F., Tan, J. et al. A CONSTANS-like transcriptional activator, OsCOL13, functions as a negative regulator of flowering downstream of OsphyB and upstream of Ehd1 in rice. Plant Mol Biol 92, 209–222 (2016). https://doi.org/10.1007/s11103-016-0506-3

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