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Enhanced drought tolerance in Arabidopsis via genetic manipulation aimed at the reduction of glucosamine-induced ROS generation

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Abstract

In animals, high glucose exerts some of its deleterious effects by activation of the hexosamine biosynthesis pathway (HBP), a branch of the glycolytic pathway that produces amino sugars (Daniels et al. in Mol Endocrinol 7:1041–1048, 1993; Du et al. in Proc Natl Acad Sci USA 97:12222–12226, 2000). Glucosamine (GlcN) is a naturally occurring amino sugar produced by amidation of fructose-6-phosphate. Previously, we observed that glucosamine (GlcN) inhibits hypocotyl elongation in Arabidopsis thaliana by a process involving the significant increase of reactive oxygen species. The present study investigated the relationship between GlcN-induced ROS generation and abiotic stress responses in Arabidopsis by generating two types of transgenic plant. Scavenging of endogenous GlcN by ectopic expression of E. coli glucosamine-6-phosphate deaminase (NagB) was observed to confer enhanced tolerance to oxidative, drought, and cold stress. Consistent with this result, overproduction of GlcN by the ectopic expression of E. coli glucosamine-6-phosphate synthase (GlmS) induced cell death at an early stage. Taken together, these data suggest that genetic manipulation of endogenous GlcN level can effectively lead to the generation of abiotic stress-tolerant transgenic crop plants.

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Acknowledgments

This work was supported by a grant from Korea Research Foundation to Hojoung Lee (grant # 2009-0065693) and in part by a grant from Korea Research Foundation to Suk-Whan Hong (grant #2009-0074729 and 2010-0020141).

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Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Korea University, 1, 5-ka Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea

    Seung Hee Chu, Ha-na Noh, Sooah Kim, Kyoung Heon Kim, Suk-Whan Hong & Hojoung Lee

  2. Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Institute, Chonnam National University, Gwangju, Korea

    Suk-Whan Hong

Authors
  1. Seung Hee Chu
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  2. Ha-na Noh
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  3. Sooah Kim
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  4. Kyoung Heon Kim
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Corresponding authors

Correspondence to Suk-Whan Hong or Hojoung Lee.

Additional information

Seung Hee Chu and Ha-na Noh contributed equally to this work.

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11103_2010_9691_MOESM1_ESM.jpg

Figure S1. Cold stress tolerance of wild-type and NagB-ox plants. (A) Plates with 7-day-old seedlings grown at room temperature were transferred to a 4°C chamber with light for 7 days. The photograph was taken at the end of the cold treatment. (B) Root lengths were measured from (A) and plotted. (JPEG 41 kb)

11103_2010_9691_MOESM2_ESM.jpg

Figure S2. Two-DE Analysis of Col-0 and NagB-ox plants subjected to glucosamine treatment. Two-week-old col-0 and NagB-ox plantlets were grown on control media and then sampled or treated for 4 h with 1 mM glucosamine. Specific proteins exhibiting large differences in expression were analyzed by MALDI-TOF (JPEG 42 kb)

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Chu, S.H., Noh, Hn., Kim, S. et al. Enhanced drought tolerance in Arabidopsis via genetic manipulation aimed at the reduction of glucosamine-induced ROS generation. Plant Mol Biol 74, 493–502 (2010). https://doi.org/10.1007/s11103-010-9691-7

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