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Proteomic analysis of blue light-induced twining response in Cuscuta australis

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Abstract

The parasitic plant Cuscuta australis (dodder) invades a variety of species by entwining the stem and leaves of a host and developing haustoria. The twining response prior to haustoria formation is regarded as the first sign for dodders to parasitize host plants, and thus has been the focus of studies on the host-parasite interaction. However, the molecular mechanism is still poorly understood. In the present work, we have investigated the different effects of blue and white light on the twining response, and identified a set of proteins that were differentially expressed in dodder seedlings using a proteomic approach. Approximately 1,800 protein spots were detected on each 2-D gel, and 47 spots with increased or decreased protein levels were selected and analyzed with MALDI-TOF-MS. Peptide mass fingerprints (PMFs) obtained for these spots were used for protein identification through cross-species database searches. The results suggest that the blue light-induced twining response in dodder seedlings may be mediated by proteins involved in light signal transduction, cell wall degradation, cell structure, and metabolism.

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References

  • Ballaré LC (1999) Keeping up with the neighbours: phytochrome sensing and other signaling mechanisms. Trends Plant Sci 4(3):97–102

    Article  PubMed  Google Scholar 

  • Bowler C, Neuhaus G, Yamagata H, Chua NH (1994) Cyclic GMP and calcium mediate phytochrome phototransduction. Cell 77:73–81

    Article  CAS  PubMed  Google Scholar 

  • Carpentier CS, Witters E, Laukens K, Van Onckelen H, Swennen R, Panis B (2007) Banana (Musa spp.) as a model to study the meristem proteome: Acclimation to osmotic stress. Proteomics 7:92–105

    Article  CAS  PubMed  Google Scholar 

  • Cui S, Huang F, Wang J, Ma X, Cheng Y, Liu J (2005) A proteomic analysis of cold stress responses in rice seedlings. Proteomics 5:3162–3172

    Article  CAS  PubMed  Google Scholar 

  • Damerval C, de Vienne D, Zivy M, Thiellement H (1986) The technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophor 7:52–54

    Article  CAS  Google Scholar 

  • Fukaki H, Wysocka-Diller J, Kato T, Fukisawa H, Benfey PN, Tasaka M (1998) Genetic evidence that the endodermis is essential for shoot gravitropism in Arabidopsis thaliana. Plant J 14:425–430

    Article  CAS  PubMed  Google Scholar 

  • Furuhashi K, Kanno M, Morita T (1995) Photocontrol of parasitism in a parasitic flowering plant, Cuscuta japonica Chois, cultured in vitro. Plant Cell Physiol 36(3):533–536

    Article  CAS  Google Scholar 

  • García-Martinez JL, Gil J (2001) Light regulation of gibberellin biosynthesis and mode of action. J Plant Growth Regul 20:354–368

    Article  PubMed  Google Scholar 

  • Gharahdaghi F, Weinberg CR, Meagher DA, Imai BS, Mische SM (1999) Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity. Electrophor 20:601–605

    Article  CAS  Google Scholar 

  • Guo HW, Todd M, Hien D, Lin CT (2001) SUB1 an Arabidopsis Ca2+-Binding protein Involved in cryptochrome and phytochrome coaction. Sci 291:487–490

    Article  CAS  Google Scholar 

  • Haidar MA (2003) Characterisation of the interaction between cryptochromes and phytochromes in blue light-induced coiling and prehaustoria development of dodder (Cuscuta campestris) seedlings. Ann Appl Biol 143:57–62

    Article  CAS  Google Scholar 

  • Higgs NH, Peterson JK (2005) Phylogenetic analysis of the Formin Homology 2 Domain. Mol Biol Cell 16:1–13

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hu F, Kong CH (2003) Parasite plants chemical recognition to host. Acta Ecol Sin 23(5):965–971

    Google Scholar 

  • Huang JZ, Li YH (1991a) A study on the developmental anatomy of haustoria in Cuscuta japonica Choisy induced by exogenous cytokinin application. J Nanjing Agric Univ 14(2):23–28

    Google Scholar 

  • Huang JZ, Li YH (1991b) Relationship between haustorial formation of Cuscuta japonica induced by exogenous cytokinin and calmodulin. Acta Bot Boreal –Occident Sin 11(2):116–170

    Google Scholar 

  • Im YJ, Perera IY, Brglez I, Davis AJ, Stevenson-Paulik J, Phillippy BQ, Johannes E, Allen NS, Boss WF (2007) Increasing plasma membrane phosphatidylinositol (4, 5) bisphosphate biosynthesis increases phosphoinositide metabolism in Nicotiana tabacum. Plant Cell 19:1603–1616

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kelly CK (1992) Resource choice in Cuscuta europaea. Proc Natl Acad Sci 89:12194–12197

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Koornneef M, Elgersma A, Hanhart CJ, van Loenen-Martinet EP, van Rijn L, Zeevaart JAD (1985) A gibberellin insensitive mutant of Arabidopsis thaliana. Physiol Plant 65:33–39

    Article  CAS  Google Scholar 

  • Lane HC, Kasperbauer MJ (1965) Photomorphogenic responses of dodder seedlings. Plant Physiol 40:109–116

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lariguet P, Schepens I, Hodgson D, Pedmale UV, Trevisan M, Kami C, de Carbonnel M, Alonso JM, Ecker JR, Liscum E, Fankhauser C (2006) Phytochrome kinase substrate 1 is a phototropin 1 binding protein required for phototropism. PNAS 103(26):10134–10139

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li WZ, Chinnappa CC (2004) Isolation and characterization of PHYC gene from Stellaria longipes: differential expression regulated by different red/far-red light ratios and photoperiods. Planta 220(2):318–330

    Article  CAS  PubMed  Google Scholar 

  • Molloy MP, Phadke ND, Maddock JR, Andrews PC (2001) Two-dimensional electrophoresis and peptide mass fingerprinting of bacterial outer membrane proteins. Electrophor 22:1686–1696

    Article  CAS  Google Scholar 

  • Mortz E, Krogh TN, Vorum H, Görg A (2001) Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time-of-flight analysis. Proteomics 1:1359–1363

    Article  CAS  PubMed  Google Scholar 

  • Nagar R, Singh M, Sanwal GB (1984) Cell-wall degrading enzymes in Cuscuta reflexa and its host. J Exp Bot 35:1104–1112

    Article  CAS  Google Scholar 

  • Neuhaus G, Bowler C, Kern R, Chua NH (1993) Calcium/Calmodulin-dependent and independent phytochrome signal transduction pathways. Cell 73:937–952

    Article  CAS  PubMed  Google Scholar 

  • Nun NB, Mayer AM (1999) Culture of pectin methylesterase and polyphenoloxidase in Cuscuta campestris. Phytochem 50:719–727

    Article  CAS  Google Scholar 

  • Nun NB, Mor A, Mayer AM (1999) A cofactor requirement for polygalacturonase from Cuscuta campestris. Phytochem 52:1217–1221

    Article  CAS  Google Scholar 

  • Orr GL, Haidar MA, Orr DA (1996) Small seed dodder (Cuscuta planiflora) phototropism toward far-red when in white light. Weed Sci 44:233–240

    Article  CAS  Google Scholar 

  • Park SO, Zheng Z, Oppenheimer DG, Hauser BA (2005) The PRETTY FEW SEEDS2 gene encodes an Arabidopsis homeodomain protein that regulates ovule development. Dev 132:841–849

    Article  CAS  Google Scholar 

  • Porubleva L, Vander Velden K, Kothari S, Oliver DJ, Chitnis PR (2001) The proteome of maize of leaves: use of gene sequences and expressed sequence tag data for identification of proteins with peptide mass fingerprints. Electrophor 22(9):1724–1738

    Article  CAS  Google Scholar 

  • Pysh LD, Wysocka-Diller JW, Camilleri C, Bouchez D, Benfey PN (1999) The GRAs gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes. Plant J 18(1):111–119

    Article  CAS  PubMed  Google Scholar 

  • Requejo R, Tena M (2006) Maize response to acute arsenic toxicity as revealed by proteome analysis of plant shoots. Proteomics 6:S156–S162

    Article  PubMed  Google Scholar 

  • Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 68:850–858

    Article  CAS  PubMed  Google Scholar 

  • Silverstone AL, Ciampaglio CN, Sun TP (1998) The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. Plant Cell 10:155–169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tada Y, Sugai M, Furuhashi K (1996) Haustoria of Cuscuta japonica, a holoparasitic flowering plant, are induced by the cooperative effects of far-red light and tactile stimuli. Plant Cell Physiol 37:1049–1053

    Article  CAS  Google Scholar 

  • van der Kooij TAW, Krause K, Dörr I, Krupinska K (2000) Molecular, functional and ultrastructural characterisation of plastids from six species of the parasitic flowering plant genus Cuscuta. Planta 210:701–707

    Article  PubMed  Google Scholar 

  • Verrills NM, Harry JH, Walsh BJ, Hains PG, Robinson ES (2000) Cross-matching marsupial proteins with eutherian mammal databases: proteome analysis of cells from UV-induced skin tumours of an opossum (Monodelphis domestica). Electrophor 21:3810–3822

    Article  CAS  Google Scholar 

  • Wang YN, Liu MY, Li X (2004) Multidimensional nature of glyceraldehyde-3-phosphate dehydrogenase in plants. Acta Bot Boreal –Occident Sin 25(3):607–614

    Google Scholar 

  • Wilkins MR, Williams KL (1997) Cross-species protein identification using amino acid composition, peptide mass fingerprinting, isoelectric point and molecular mass: a theoretical evaluation. J Theor Biol 186:7–15

    Article  CAS  PubMed  Google Scholar 

  • Xu YY, Chong K (2005) Progress in research on plant stem cell organizer gene WUSCHEL. J Plant Physiol Mol Biol 31(5):461–468

    CAS  Google Scholar 

  • Yamagata CB, Neuhaus G, Chua NH (1994) Phytochrome signal transduction pathways are regulated by reciprocal control mechanisms. Genes Dev 8:2188–2202

    Article  PubMed  Google Scholar 

  • Zhao XY, Yu XH, Liu XM, Lin CT (2007) Light regulation of gibberellins metabolism in seedling development. J Integr Plant Biol 49(1):21–27

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank You Shang for the design and construction of blue light sources, and Xiaochun Wang for measuring the optical and electric parameters of light-emitting diodes.

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Authors and Affiliations

  1. School of Life Science, Xiamen University, 361005, Xiamen, China

    Dongxiao Li, Xiaopo Yang, Guoguang Zhang & Liang Chen

  2. Department of Genetics & Biochemistry, Clemson University, Clemson, SC, 29634, USA

    Liangjiang Wang

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  1. Dongxiao Li
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  2. Liangjiang Wang
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  3. Xiaopo Yang
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  4. Guoguang Zhang
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  5. Liang Chen
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Correspondence to Liang Chen.

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Li, D., Wang, L., Yang, X. et al. Proteomic analysis of blue light-induced twining response in Cuscuta australis. Plant Mol Biol 72, 205–213 (2010). https://doi.org/10.1007/s11103-009-9562-2

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  • DOI: https://doi.org/10.1007/s11103-009-9562-2

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