Skip to main content
SpringerLink
Log in

Genomic and functional characterization of StCDPK1

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is located in the distal portion of chromosome 12. Western blot and immunolocalization assays (using confocal and transmission electron microscopy) performed with a specific antibody against StCDPK1 indicate that this kinase is mainly located in the plasma membrane of swelling stolons and sprouting tubers. Sucrose (4–8%) increased StCDPK1 protein content in non-induced stolons, however the amount detected in swelling stolons was higher. Transgenic lines with reduced expression of StCDPK1 (β7) did not differ from controls when cultured under multiplication conditions, but when grown under tuber inducing conditions some significant differences were observed: the β7 line tuberized earlier than controls without the addition of CCC (GA inhibitor), developed more tubers than wild type plants in the presence of hormones that promote tuberization in potato (ABA and BAP) and was more insensitive to GA action (stolons were significantly shorter than those of control plants). StCDPK1 expression was induced by GA, ABA and BAP. Our results suggest that StCDPK1 plays a role in GA-signalling and that this kinase could be a converging point for the inhibitory and promoting signals that influence the onset of potato tuberization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Abbasi F, Onodera H, Toki S, Tanaka H, Komatsu S (2004) OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath. Plant Mol Biol 55:541–552. doi:10.1007/s11103-004-1178-y

    Article  PubMed  CAS  Google Scholar 

  • Abo-el-Saad M, Wu R (1995) A rice membrane calcium-dependent protein kinase is induced by gibberellin. Plant Physiol 108:787–793. doi:10.1104/pp.108.2.787

    Article  PubMed  CAS  Google Scholar 

  • Achard P, Vriezen WH, Van Der Straeten D, Harberd NP (2003) Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function. Plant Cell 15:2816–2825. doi:10.1105/tpc.015685

    Article  PubMed  CAS  Google Scholar 

  • Asano T, Tanaka N, Yang G, Hayashi N, Komatsu S (2005) Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice. Plant Cell Physiol 46:356–366. doi:10.1093/pcp/pci035

    Article  PubMed  CAS  Google Scholar 

  • Bachmann M, Shiraishi N, Campbell WH, Yoo BC, Harmon AC, Huber SC (1996) Identification of the major regulatory phosphorylation site as Ser-543 in spinach leaf nitrate reductase and its phosphorylation by a calcium-dependent protein kinase in vitro. Plant Cell 8:505–517

    Article  PubMed  CAS  Google Scholar 

  • Balamani V, Veluthambi K, Poovaiah BW (1986) Effect of calcium on tuberization in potato. Plant Physiol 80:856–858

    Article  PubMed  CAS  Google Scholar 

  • Bonierbale MW, Plaisted RL, Pineda O, Tanksley SD (1994) QTL analysis of trichome-mediated insect resistance in potato. Theor Appl Genet 87:973–987. doi:10.1007/BF00225792

    Article  CAS  Google Scholar 

  • Busino L, Donzelli M, Chiesa M, Guardavaccaro D, Ganoth D, Dorello NV, Hershko A, Pagano M, Draetta GF (2003) Degradation of Cdc25A by b-TrCP during S phase and in response to DNA damage. Nature 426:87–91. doi:10.1038/nature02082

    Article  PubMed  CAS  Google Scholar 

  • Carrera E, Bou J, Garcia-Martinez JL, Prat S (2000) Changes in GA 20-oxidase gene expression strongly affect stem length, tuber induction and tuber yield of potato plants. Plant J 22:247–256. doi:10.1046/j.1365-313x.2000.00736.x

    Article  PubMed  CAS  Google Scholar 

  • Cheng S-H, Willmann MR, Chen H-C, Sheen J (2002) Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129:469–485. doi:10.1104/pp.005645

    Article  PubMed  CAS  Google Scholar 

  • Cutter EG (1978) Structure and development of the potato plant. In: Harris PM (ed) The potato crop. Halsted Press, Wiley, New York, pp 70–152

    Google Scholar 

  • de Lucas M, Davière JM, Rodríguez-Falcón M, Pontin M, Iglesias-Pedraz JM, Lorrain S, Fankhauser C, Blázquez MA, Titarenko E, Prat S (2008) A molecular framework for light and gibberellin control of cell elongation. Nature 451:480–484. doi:10.1038/nature06520

    Article  PubMed  Google Scholar 

  • Dill A, Thomas SG, Hu J, Steber CM, Sun TP (2004) The Arabidopsis Fbox protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. Plant Cell 16:1392–1405. doi:10.1105/tpc.020958

    Article  PubMed  CAS  Google Scholar 

  • Evans NH, McAinsh MR, Hetherington AM (2001) Calcium oscillations in higher plants. Curr Opin Plant Biol 4:415–420. doi:10.1016/S1369-5266(00)00194-1

    Article  PubMed  CAS  Google Scholar 

  • Ewing EE, Struik PC (1992) Tuber formation in potato: induction, initiation and growth. Hortic Rev (Am Soc Hortic Sci) 14:89–197

    Google Scholar 

  • Fan LM, Zhao ZX, Assmann SM (2004) Guard cells: a dynamic signaling model. Curr Opin Plant Biol 7:537–546. doi:10.1016/j.pbi.2004.07.009

    Article  PubMed  CAS  Google Scholar 

  • Feingold SE, Lloyd J, Norero N, Bonierbale M, Lorenzen J (2005) Map location and diversity values for new potato SSRs developed from EST databases. Theor Appl Genet 111:456–466. doi:10.1007/s00122-005-2028-2

    Article  PubMed  CAS  Google Scholar 

  • Fernie AR, Willmitzer L (2001) Molecular and biochemical triggers of potato tuber development. Plant Physiol 127:1459–1465. doi:10.1104/pp.010764

    Article  PubMed  CAS  Google Scholar 

  • Finkelstein RR, Gampala S, Rock C (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14:S15–S45

    PubMed  CAS  Google Scholar 

  • Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375

    CAS  Google Scholar 

  • Fu X, Harberd NP (2003) Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421:740–743. doi:10.1038/nature01387

    Article  PubMed  CAS  Google Scholar 

  • Fu X, Richards DE, Fleck B, Xie D, Burton N, Harberd NP (2004) The Arabidopsis Mutant sleepy1gar2–1 protein promotes plant growth by increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA protein substrates. Plant Cell 16:1406–1418. doi:10.1105/tpc.021386

    Article  PubMed  CAS  Google Scholar 

  • Furuichi T, Muto S (2005) H+-Coupled sugar transporter, an initiator of sugar-induced Ca2+-signaling in plant cells. Z Naturforsch 60:764–768

    CAS  Google Scholar 

  • Gargantini P, Gonzalez-Rizzo S, Chinchilla D, Raices M, Giammaria V, Ulloa R, Frugier F, Crespi M (2006) A CDPK isoform regulates nodule number in Medicago truncatula. Plant J 48:843–856. doi:10.1111/j.1365-313X.2006.02910.x

    Article  PubMed  CAS  Google Scholar 

  • Garner N, Blake J (1989) The induction and development of potato microtubers in vitro on media free of growth regulating substances. Ann Bot (Lond) 63:663–674

    CAS  Google Scholar 

  • Gibson SI (2004) Sugar and phytohormone response pathways: navigating a signalling network. J Exp Bot 55:253–264. doi:10.1093/jxb/erh048

    Article  PubMed  CAS  Google Scholar 

  • Gilroy S, Jones RL (1992) Gibberellic acid and abscisic acid coordinately regulate cytoplasmic calcium and secretory activity in barley aleurone protoplasts. Proc Natl Acad Sci USA 89:3591–3595. doi:10.1073/pnas.89.8.3591

    Article  PubMed  CAS  Google Scholar 

  • Gonzalo S, Linder ME (1998) SNAP-25 palmitoylation and plasma membrane targeting require a functional secretory pathway. Mol Biol Cell 9:585–597

    PubMed  CAS  Google Scholar 

  • Harmon AC (2003) Calcium-regulated protein kinases of plants. Gravit Space Biol Bull 16:83–90

    PubMed  Google Scholar 

  • Harris N (1994) Immunocytochemistry for light and electron microscopy. In: Harris S, Oparka KJ (eds) Plant cell biology. Oxford University Press, Oxford, pp 157–176

    Google Scholar 

  • Himmelbach A, Yang Y, Grill E (2003) Relay and control of abscisic acid signaling. Curr Opin Plant Biol 6:470–479. doi:10.1016/S1369-5266(03)00090-6

    Article  PubMed  CAS  Google Scholar 

  • Hrabak EM, Chan CW, Gribskov M, Harper JF, Choi JH, Halford N, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132:666–680. doi:10.1104/pp.102.011999

    Article  PubMed  CAS  Google Scholar 

  • Jackson SD (1999) Multiple signaling pathways controls tuber induction in potato. Plant Physiol 119:1–8. doi:10.1104/pp.119.1.1

    Article  PubMed  CAS  Google Scholar 

  • Jackson PK, Eldridge AG (2002) The SCF ubiquitin ligase: an extended look. Mol Cell 9:923–925. doi:10.1016/S1097-2765(02)00538-5

    Article  PubMed  CAS  Google Scholar 

  • Khan MM, Jan A, Karibe H, Komatsu S (2005) Identification of phosphoproteins regulated by gibberellin in rice leaf sheath. Plant Mol Biol 58:27–40. doi:10.1007/s11103-005-4013-1

    Article  PubMed  CAS  Google Scholar 

  • Kim MS, Kim HS, Kim YS, Baek KH, Oh HW, Hahn KW, Bae RN, Lee IJ, Joung H, Jeon JH (2007) Superoxide anion regulates plant growth and tuber development of potato. Plant Cell Rep 26:1717–1725. doi:10.1007/s00299-007-0380-1

    Article  PubMed  CAS  Google Scholar 

  • Klimecka M, Muszyńska G (2007) Structure and functions of plant calcium-dependent protein kinases. Acta Biochim Pol 54:219–233

    PubMed  CAS  Google Scholar 

  • Kloosterman B, Navarro C, Bijsterbosch G, Lange T, Prat S, Visser RG, Bachem CW (2007) StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development. Plant J 52:362–373. doi:10.1111/j.1365-313X.2007.03245.x

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi M, Ohura I, Kawakita K, Yokota N, Fujiwara M, Shimamoto K, Doke N, Yoshioka H (2007) Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase. Plant Cell 19:1065–1080. doi:10.1105/tpc.106.048884

    Article  PubMed  CAS  Google Scholar 

  • Krauss A (1985) Interaction of nitrogen nutrition, phytohormones and tuberization. In: Li PH (ed) Potato physiology. Academic Press, London, pp 209–231

    Google Scholar 

  • Larsson C, Sommarin M, Widell S (1994) Isolation of highly purified plant plasma membranes and separation of inside-out and right-side-out vesicles. Methods Enzymol 228:451–469. doi:10.1016/0076-6879(94)28046-0

    Article  CAS  Google Scholar 

  • Lee SS, Cho HS, Yoon GM, Ahn J-W, Kim HH, Pai HS (2003) Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum. Plant J 33:825–840. doi:10.1046/j.1365-313X.2003.01672.x

    Article  PubMed  CAS  Google Scholar 

  • Lee SS, Yoon GM, Rho EJ, Moon E, Pai H-S (2006) Functional characterization of NtCDPK1 in tobacco. Mol Cells 21:141–146

    PubMed  CAS  Google Scholar 

  • Li A-L, Zhu Y-F, Tan X-M, Wang X, Wei B, Guo H-Z, Zhang Z-L, Chen X-B, Zhao G-Y, Kong X-Y, Jia J-Z, Mao L (2008) Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.). Plant Mol Biol 66:429–443. doi:10.1007/s11103-007-9281-5

    Article  PubMed  CAS  Google Scholar 

  • Lu SX, Hrabak EM (2002) An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum. Plant Physiol 128:1008–1021. doi:10.1104/pp.010770

    Article  PubMed  CAS  Google Scholar 

  • Lu B, Ding R, Zhang L, Yu X, Huang B, Chen W (2006) Molecular cloning and characterization of a novel calcium-dependent protein kinase gene IiCPK2 responsive to polyploidy from tetraploid Isatis indigotica. J Biochem Mol Biol 39:607–617

    PubMed  CAS  Google Scholar 

  • Ludwig AA, Romeis T, Jones JD (2004) CDPK-mediated signalling pathways: specificity and cross-talk. J Exp Bot 55:181–188. doi:10.1093/jxb/erh008

    Article  PubMed  CAS  Google Scholar 

  • MacIntosh GC, Ulloa RM, Raíces M, Téllez-Iñón MT (1996) Changes in calcium-dependent protein kinase activity during in vitro tuberization in potato. Plant Physiol 112:1541–1550

    PubMed  CAS  Google Scholar 

  • Martínez-García JF, Virgós-Soler A, Prat S (2002) Control of photoperiod-regulated tuberization in potato by the Arabidopsis flowering-time gene CONSTANS. Proc Natl Acad Sci USA 99:15211–15216. doi:10.1073/pnas.222390599

    Article  PubMed  Google Scholar 

  • McCubbin AG, Ritchie SM, Swanson SJ, Gilroy S (2004) The calcium-dependent protein kinase HvCDPK1 mediates the gibberellic acid response of the barley aleurone through regulation of vacuolar function. Plant J 39:206–218. doi:10.1111/j.1365-313X.2004.02121.x

    Article  PubMed  CAS  Google Scholar 

  • McGinnis KM, Thomas SG, Soule JD, Strader LC, Zale JM, Sun TP, Steber CM (2003) The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase. Plant Cell 15:1120–1130. doi:10.1105/tpc.010827

    Article  PubMed  CAS  Google Scholar 

  • Menzel CM (1983) Tuberization in potato at high temperatures: gibberellin content and transport from buds. Ann Bot (Lond) 52:697–702

    CAS  Google Scholar 

  • Menzel CM (1985) Tuberization in potato at high temperatures: interaction between temperature and irradiance. Ann Bot (Lond) 55:35–39

    CAS  Google Scholar 

  • Mori IC, Murata Y, Yang Y, Munemasa S, Wang Y-F, Andreoli S, Tiriac H, Alonso JM, Harper JF, Ecker JR, Kwak JM, Schroeder JI (2006) CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca2+-permeable channels and stomatal closure. PLoS Biol 4:e327. doi:10.1371/journal.pbio.0040327

    Article  PubMed  Google Scholar 

  • Müller-Röber B, Sonnewald U, Willmitzer L (1992) Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J 11:1229–1238

    PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325. doi:10.1093/nar/8.19.4321

    Article  PubMed  CAS  Google Scholar 

  • Nakajima M, Shimada A, Takashi Y, Kim YC, Park SH, Ueguchi-Tanaka M, Suzuki H, Katoh E, Iuchi S, Kobayashi M, Maeda T, Matsuoka M, Yamaguchi I (2006) Identification and characterization of Arabidopsis gibberellin receptors. Plant J 46:880–889. doi:10.1111/j.1365-313X.2006.02748.x

    Article  PubMed  CAS  Google Scholar 

  • Navarro-Lérida I, Alvarez-Barrientos A, Gavilanes F, Rodriguez-Crespo I (2002) Distance-dependent cellular palmitoylation of de-novo-designed sequences and their translocation to plasma membrane subdomains. J Cell Sci 115:3119–3130

    PubMed  Google Scholar 

  • Patton EE, Willems AR, Tyers M (1998) Combinatorial control in ubiquitin-dependent proteolysis: don’t Skp the F-box hypothesis. Trends Genet 14:236–243. doi:10.1016/S0168-9525(98)01473-5

    Article  PubMed  CAS  Google Scholar 

  • Perl A, Aviv D, Galun E (1991) Nuclear–organelle interaction in Solanum: interspecific hybridizations and their correlation with a plastome dendrogram. Mol Gen Genet 228:193–200

    PubMed  CAS  Google Scholar 

  • Prat S, Frommer WB, Hofgen R, Keil M, Kossmann J, Koster-Topfer M, Liu XJ, Muller B, Peña-Cortés H, Rocha-Sosa M, Sanchez-Serrano JJ, Sonnewald U, Willmitzer L (1990) Gene expression during tuber development in potato plants. FEBS Lett 268:334–338. doi:10.1016/0014-5793(90)81281-R

    Article  PubMed  CAS  Google Scholar 

  • Raices M, Chico JM, Tellez-Iñón MT, Ulloa RM (2001) Molecular characterization of StCDPK1, a calcium-dependent protein kinase from Solanum tuberosum that is induced at the onset of tuber development. Plant Mol Biol 46:591–601. doi:10.1023/A:1010661304980

    Article  PubMed  CAS  Google Scholar 

  • Raices M, Gargantini PR, Chinchilla D, Crespi M, Tellez-Iñón MT, Ulloa RM (2003a) Regulation of CDPK isoforms during tuber development. Plant Mol Biol 52:1011–1024. doi:10.1023/A:1025478315648

    Article  PubMed  CAS  Google Scholar 

  • Raices M, Ulloa RM, MacIntosh GC, Crespi M, Tellez-Iñón MT (2003b) StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration. J Exp Bot 54:2589–2591. doi:10.1093/jxb/erg282

    Article  PubMed  CAS  Google Scholar 

  • Raices M, MacIntosh GC, Ulloa RM, Gargantini PR, Vozza NF, Tellez-Iñón MT (2003c) Sucrose increases calcium-dependent protein kinase and phosphatase activities in potato plants. Cell Mol Biol 49:959–964

    PubMed  CAS  Google Scholar 

  • Ray S, Agarwal P, Arora R, Kapoor S, Tyagi AK (2007) Expression analysis of calcium-dependent protein kinase gene family during reproductive development and abiotic stress conditions in rice (Oryza sativa L. ssp. indica). Mol Genet Genomics 278:493–505. doi:10.1007/s00438-007-0267-4

    Article  PubMed  CAS  Google Scholar 

  • Rodríguez-Falcón M, Bou J, Prat S (2006) Seasonal control of tuberization in potato: conserved elements with the flowering response. Annu Rev Plant Biol 57:151–180. doi:10.1146/annurev.arplant.57.032905.105224

    Article  PubMed  Google Scholar 

  • Rosin FM, Hart JK, Horner HT, Davies PJ, Hannapel DJ (2003) Overexpression of a knox gene of potato alters vegetative development by decreasing gibberellin accumulation. Plant Physiol 132:106–111. doi:10.1104/pp.102.015560

    Article  PubMed  CAS  Google Scholar 

  • Ross JJ, Reid JB, Swain SM, Hasan O, Poole AT, Hedden P, Willis CL (1995) Genetic regulation of gibberellin deactivation in Pisum. Plant J 7:513–523. doi:10.1046/j.1365-313X.1995.7030513.x

    Article  CAS  Google Scholar 

  • Sarkar D (2008) The signal transduction pathways controlling in planta tuberization in potato: an emerging synthesis. Plant Cell Rep 27:1–8. doi:10.1007/s00299-007-0457-x

    Article  PubMed  CAS  Google Scholar 

  • Sasaki A, Itoh H, Gomi K, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Jeong DH, An G, Kitano H, Ashikari M, Matsuoka M (2003) Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science 299:1896–1898. doi:10.1126/science.1081077

    Article  PubMed  CAS  Google Scholar 

  • Shapiro MB, Senapathy P (1987) RNA splice junctions of different classes of eukaryotes; sequence statistics and functional implications in gene expression. Nucleic Acids Res 15:7155–7174. doi:10.1093/nar/15.17.7155

    Article  PubMed  CAS  Google Scholar 

  • Sharma A, Komatsu S (2002) Involvement of a Ca(2+)-dependent protein kinase component downstream to the gibberellin-binding phosphoprotein, RuBisCO activase, in rice. Biochem Biophys Res Commun 290:690–695. doi:10.1006/bbrc.2001.6269

    Article  PubMed  CAS  Google Scholar 

  • Simko I (1994) Sucrose application causes hormonal changes associated with potato tuber induction. J Plant Growth Regul 13:73–77. doi:10.1007/BF00210950

    Article  CAS  Google Scholar 

  • Smotrys JE, Linder ME (2004) Palmitoylation of intracellular signaling proteins: regulation and function. Annu Rev Biochem 73:559–587. doi:10.1146/annurev.biochem.73.011303.073954

    Article  PubMed  CAS  Google Scholar 

  • Sonnewald U, Hajirezaei MR, Kossmann J, Heyer A, Trethewey RN, Willmitzer L (1997) Increased potato tuber size resulting from apoplastic expression of a yeast invertase. Nat Biotechnol 15:794–797. doi:10.1038/nbt0897-794

    Article  PubMed  CAS  Google Scholar 

  • Struik PC, Boon EJ, Vreugdenhil D (1987) Effects of extracellular extracts from leaves on the tuberization of cuttings of potato (Solanum tuberosum L.). Plant Physiol 84:214–217

    Article  PubMed  Google Scholar 

  • Thomas SG, Phillips AL, Hedden P (1999) Molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation. Proc Natl Acad Sci USA 96:4698–4703. doi:10.1073/pnas.96.8.4698

    Article  PubMed  CAS  Google Scholar 

  • Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, Matsuoka M (2005) Gibberellin insensitive dwarf1 encodes a soluble receptor for gibberellin. Nature 437:693–698. doi:10.1038/nature04028

    Article  PubMed  CAS  Google Scholar 

  • Visser RGF, Vreugdenhil D, Hendriks T, Jacobsen EJ (1994) Gene expression and carbohydrate content during stolon to tuber transition in potatoes (Solanum tuberosum). Physiol Plant 90:285–292. doi:10.1111/j.1399-3054.1994.tb00389.x

    Article  CAS  Google Scholar 

  • Vreugdenhil D, Sergeeva LI (1999) Gibberellins and tuberization in potato. Potato Res 42:471–481. doi:10.1007/BF02358163

    Article  CAS  Google Scholar 

  • Willige BC, Ghosh S, Nill C, Zourelidou M, Dohmann EM, Maier A, Schwechheimer C (2007) The DELLA domain of GA INSENSITIVE mediates the interaction with the GA INSENSITIVE DWARF1A gibberellin receptor of Arabidopsis. Plant Cell 19:1209–1220. doi:10.1105/tpc.107.051441

    Article  PubMed  CAS  Google Scholar 

  • Woolley DJ, Wareing PF (1972) Environmental effects on endogenous cytokinins and gibberellin levels in Solanum tuberosum. New Phytol 71:1015–1025. doi:10.1111/j.1469-8137.1972.tb01979.x

    Article  CAS  Google Scholar 

  • Xu X, van Lammeren AA, Vermeer E, Vreugdenhil D (1998) The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol 117:575–584. doi:10.1104/pp.117.2.575

    Article  PubMed  CAS  Google Scholar 

  • Yoon GM, Cho HS, Ha HJ, Liu JR, Lee HP (1999) Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein. Plant Mol Biol 39:991–1001. doi:10.1023/A:1006170512542

    Article  PubMed  CAS  Google Scholar 

  • Yuan X, Deng KQ, Zhao XY, Wu XJ, Qin YZ, Tang DY, Liu XM (2007) A calcium-dependent protein kinase is involved in plant hormone signal transduction in Arabidopsis. J Plant Physiol Mol Biol 33:227–234

    CAS  Google Scholar 

  • Zhang M, Liang S, Lu YT (2005) Cloning and functional characterization of NtCPK4, a new tobacco calcium-dependent protein kinase. Biochim Biophys Acta 1729:174–185

    PubMed  CAS  Google Scholar 

  • Zhu S-Y, Yu X-C, Wang X-J, Zhao R, Li Y, Fan R-C, Shang Y, Du S-Y, Wang X-F, Wu F-Q, Xu Y-H, Zhang X-Y, Zhang D-P (2007) Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis. Plant Cell 19:3019–3036. doi:10.1105/tpc.107.050666

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr S. Prat Monguío (CSIC, Spain) for the Ga2-oxidase and the GA20-oxidase clones and Dr Mark Taylor for the Solanum tuberosum var. Desireé genomic library. This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the University of Buenos Aires, Argentina and the Agencia Nacional de Promoción Científica y Tecnológica (FONCYT-ANPCYT) and the USDA-SCRP-FAS project: A biotechnology tool to broaden the genetic base of potato in a cooperative framework. We thank the technical work of Silvina Divito (INTA, Balcarce). RMU and SM are members of the National Research Council, CONICET. PRG, VG and CG are fellows from CONICET.

Author information

Author notes

  1. Pablo Rubén Gargantini

    Present address: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Sao Paulo, Brazil

Authors and Affiliations

  1. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, piso 2, 1428, Buenos Aires, Argentina

    Pablo Rubén Gargantini, Verónica Giammaria, Carolina Grandellis & Rita María Ulloa

  2. Laboratorio de Agro-Biotecnología, INTA-EEA Balcarce, CC276, 7620, Balcarce, Argentina

    Sergio E. Feingold

  3. Departamento de Biodiversidad y Biología Experimental, Lab. 17 Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA, Buenos Aires, Argentina

    Sara Maldonado

Authors
  1. Pablo Rubén Gargantini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Verónica Giammaria
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carolina Grandellis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sergio E. Feingold
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sara Maldonado
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rita María Ulloa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rita María Ulloa.

Additional information

The StCDPK1 codifying and genomic sequences have the GenBank Database accession numbers AF115406, and DQ507862.1 respectively.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gargantini, P.R., Giammaria, V., Grandellis, C. et al. Genomic and functional characterization of StCDPK1. Plant Mol Biol 70, 153–172 (2009). https://doi.org/10.1007/s11103-009-9462-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11103-009-9462-5

Keywords

Search

Navigation