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Mitochondrial acyl carrier proteins in Arabidopsis thaliana are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I

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Abstract

Arabidopsis mitochondria are predicted to contain three acyl carrier proteins (ACPs). These small proteins are involved in fatty acid and lipoic acid synthesis in other organisms and have been previously reported to be subunits of respiratory Complex I in mitochondria in mammals, fungi and plants. Recently, the mammalian mitochondrial ACP (mtACP) has been shown to be largely a soluble matrix protein but also to be minimally associated with Complex I (Cronan et al. 2005), consistent with its involvement in synthesis of lipoic acid for TCA cycle decarboxylating dehydrogenases in the matrix but contrary to earlier claims it was primarily a Complex I subunit. We have investigated the localization of the ACPs in Arabidopsis mitochondria. Evidence is presented that mtACP1 and mtACP2 dominate the ACP composition in Arabidopsis mitochondria, and both are present in the mitochondrial matrix rather than in the membrane. No significant amounts of mtACPs were detected in Complex I isolated by blue native gel electrophoresis, rather mtACPs were detected at low molecular mass in the soluble fraction, showing that in A. thaliana mtACPs are predominately free soluble matrix proteins.

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Abbreviations

ACP:

Acyl carrier protein

MS/MS:

Tandem mass spectrometry

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Acknowledgements

This work was funded through grants from the Australian Research Council (ARC) Centre of Excellence Programme to AHM. AHM is funded as an ARC Australian Professorial Fellow and JLH as an ARC Australian Post-doctoral Research Fellow.

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  1. ARC Center of Excellence in Plant Energy Biology, University of Western Australia, 4th Floor MCS Building M316, 35 Stirling Highway, 6009, Crawley, WA, Australia

    Etienne H. Meyer, Joshua L. Heazlewood & A. Harvey Millar

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  1. Etienne H. Meyer
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  2. Joshua L. Heazlewood
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  3. A. Harvey Millar
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Correspondence to A. Harvey Millar.

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Meyer, E.H., Heazlewood, J.L. & Millar, A.H. Mitochondrial acyl carrier proteins in Arabidopsis thaliana are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I. Plant Mol Biol 64, 319–327 (2007). https://doi.org/10.1007/s11103-007-9156-9

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