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Differential expression of genes encoding 1-aminocyclopropane-1-carboxylate synthase in Arabidopsis during hypoxia

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Abstract

Ethylene plays an essential role in response to hypoxic stress in plants. In most plant species, 1-aminocyclopropane-1-carboxylate synthase (ACS) is the key enzyme that regulates the production of ethylene. We examined the expression of ACS genes in Arabidopsis during hypoxia. Our data showed that the expression of 4 of the 12 Arabidopsis ACS genes, ACS2, ACS6, ACS7, and ACS9, is induced during hypoxia with three distinct patterns. The hypoxic induction of ACS9 is inhibited by aminooxy acetic acid, an inhibitor of ethylene biosynthesis. In addition, the hypoxic induction of ACS9 is also reduced in etr1-1 and ein2-1, two ethylene insensitive mutants in ethylene-signaling pathways, whereas the addition of 1-aminocyclopropane-1-carboxylic acid, a direct precursor of ethylene, does not induce ACS9 under normoxic conditions. These results indicate that ethylene is needed, but not sufficient, for the induction of ACS9 during hypoxia. This pattern of regulation is similar to that of ADH that encodes alcohol dehydrogenase, which we have reported previously. In contrast, the increased ethylene production during hypoxia has an inhibitory effect on ACS2 induction in roots, whereas ethylene has no effect on the hypoxic induction of ACS6 and ACS7. Based on these results, we propose that two signaling pathways are triggered during hypoxia. One pathway leads to the activation of ACS2, ACS6, and ACS7, whereas the other pathway leads to the activation of ADH and ACS9.

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

ACS:

ACC synthase

ADH:

alcohol dehydrogenase

AOA:

aminooxy acetic acid

RT:

reverse transcriptase

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Authors and Affiliations

  1. Department of Biological Sciences, University of Iowa, Iowa City, Iowa, 52242, USA

    Hsiao-Ping Peng, Ter-Yun Lin & Ming-Che Shih

  2. Department of Plant Biology and Ecology, Nankai University, Tianjing, 300071, P.R. China

    Ning-Ning Wang

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  1. Hsiao-Ping Peng
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  2. Ter-Yun Lin
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Correspondence to Ming-Che Shih.

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Peng, HP., Lin, TY., Wang, NN. et al. Differential expression of genes encoding 1-aminocyclopropane-1-carboxylate synthase in Arabidopsis during hypoxia. Plant Mol Biol 58, 15–25 (2005). https://doi.org/10.1007/s11103-005-3573-4

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