Skip to main content

Advertisement

SpringerLink
Log in

Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray*

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Somatic embryogenesis (SE) is the developmental reprogramming of somatic cells toward the embryogenesis pathway and is a notable illustration of cell totipotency. To identify genes involved in SE, subtractive polymerase chain reaction (PCR) was performed to generate transcripts highly enriched for SE-related genes, using cDNA prepared from a mixture of embryogenic callus and preglobular somatic embryos, as the tester, and cDNA from nonembryogenic callus, as the driver. After differential screening and subsequent confirmation by reverse Northern blot analysis, a total of 671 differentially expressed cDNA fragments were identified, and 242 unigenes significantly up-regulated during cotton SE were recovered, as confirmed by Northern blot and reverse-transcription PCR analysis of representative cases, including most previously published SE-related genes in plants. In total, more than half had not been identified previously as SE-related genes, including dominant crucial genes involved in transcription, posttranscription, and transportation, and about one-third had not been reported previously to GenBank or were expected to be unknown, or newly identified genes. We used cDNA arrays to further investigate the expression patterns of these genes in differentiating gradient culture, ranging from proembryogenic masses to somatic embryos at every stage. The cDNA collection is composed of a broad repertoire of SE genes which is an important resource for understanding the genetic interactions underlying SE signaling and regulation. Our results suggested that a complicated and concerted mechanism involving multiple cellular pathways is responsible for cotton SE. This report represents a systematic and comprehensive analysis of genes involved in the process of somatic embryogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CE:

cotyledonary-stage embryo

EC:

embryogenic callus

EST:

expressed sequence tag

GE:

globular embryo

GO:

gene ontology

HE:

heart embryo

LTP:

lipid-transfer protein

NEC:

nonembryogenic callus

PEC:

primary embryogenic callus

PEM:

proembryogenic masses

PGE:

preglobular embryo

PPR:

pentatricopeptide repeat

RT-PCR:

reverse-transcription-polymerase chain reaction

SCR:

SCARECROW

SE:

somatic embryogenesis

SSH:

suppression subtractive hybridization

TE:

torpedo embryo

ZF-HD:

zinc finger homeodomain

References

  • A.B. Arpat M. Waugh J.P. Sullivan M. Gonzales D. Frisch D. Main T. Wood A. Leslie R.A. Wing T.A Wilkins (2004) ArticleTitleFunctional genomics of cell elongation in developing cotton fibers Plant Mol. Biol. 54 911–929 Occurrence Handle1:CAS:528:DC%2BD2cXhtVGjurnI Occurrence Handle15604659 Occurrence Handle10.1007/s11103-004-0392-y

    Article  CAS  PubMed  Google Scholar 

  • J.H. Benedict D.W. Altman (2001) Commercialization of transgenic cotton expressing insecticidal crystal protein J.N. Jenkins S. Saha (Eds) Genetic Improvement of Cotton Science Publishers Inc Enfield, NH 137–201

    Google Scholar 

  • M. Bevan I. Bancroft E. Bent K. Love H. Goodman C. Dean R. Bergkamp W. Dirkse Staveren ParticleVan M. W. Stiekema et al. (1998) ArticleTitleAnalysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana Nature 391 485–488 Occurrence Handle1:CAS:528:DyaK1cXhtVCjtr4%3D Occurrence Handle9461215 Occurrence Handle10.1038/35140

    Article  CAS  PubMed  Google Scholar 

  • R. Chasan (1992) ArticleTitleIn the realm of the homeodomain Plant Cell 4 237–240 Occurrence Handle1:STN:280:By2A287isV0%3D Occurrence Handle1354003

    CAS  PubMed  Google Scholar 

  • A. Chugh P. Khurana (2002) ArticleTitleGene expression during somatic embryogenesis: recent advances Curr. Sci. 83 715–730 Occurrence Handle1:CAS:528:DC%2BD38Xot1Wis7o%3D

    CAS  Google Scholar 

  • M.A. Coca C. Almoguera J. Jordano (1994) ArticleTitleDevelopmental regulation of a gene coding for a low-molecular-weight heat shock protein during haustorium formation in the seedlings of a holoparasitic plant, Cuscuta japonica Plant Mol. Biol. 25 479–492 Occurrence Handle1:CAS:528:DyaK2cXmt1Kms7o%3D Occurrence Handle8049372 Occurrence Handle10.1007/BF00043876

    Article  CAS  PubMed  Google Scholar 

  • R.J. Cyr M.M. Bustos M.J. Guiltinan D.E. Fosket (1987) ArticleTitleDevelopmental modulation of tubulin protein and mRNA levels during somatic embryogenesis in cultured carrot cells Planta 171 365–376 Occurrence Handle1:CAS:528:DyaL2sXltFakur0%3D Occurrence Handle10.1007/BF00398682

    Article  CAS  Google Scholar 

  • G.H. Davidonis R.H. Hamilton (1983) ArticleTitlePlant regeneration from callus tissue of Gossypium hirsutum L Plant Sci. Lett. 32 89–93 Occurrence Handle1:CAS:528:DyaL2cXmsFWktw%3D%3D

    CAS  Google Scholar 

  • L. Diatchenko Y.F. Lau A.P. Campbell A. Chenchik F. Moqadam B. Huang S. Lukyanov K. Lukyanov N. Gurskaya E.D. Sverdlov P.D. Siebert (1996) ArticleTitleSuppression subtractive hybridization: a method for generating differentially regulated or tissue specific cDNA probes and libraries Proc. Natl. Acad. Sci. USA 93 6025–6030 Occurrence Handle1:CAS:528:DyaK28Xjs1Ojsbk%3D Occurrence Handle8650213 Occurrence Handle10.1073/pnas.93.12.6025

    Article  CAS  PubMed  Google Scholar 

  • L. Di Laurenzio J. Wysockadiller J.E. Malamy L. Pysh Y. Helariutta G. Freshour M.G. Hahn K.A. Feldmann P.N. Benfey (1996) ArticleTitleThe SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root Cell 86 423–433 Occurrence Handle1:CAS:528:DyaK28XltVSksLw%3D Occurrence Handle8756724

    CAS  PubMed  Google Scholar 

  • V.L. Dodeman G. Ducreux M. Kreis (1997) ArticleTitleZygotic embryogenesis versus somatic embryogenesis J. Exp. Bot. 48 1493–1509 Occurrence Handle1:CAS:528:DyaK2sXmsVCrsrk%3D Occurrence Handle10.1093/jexbot/48.313.1493

    Article  CAS  Google Scholar 

  • R. Galland B. Randoux J. Vasseur J.L. Hilbert (2001) ArticleTitleA glutathione S-transferase cDNA identified by mRNA differential display is upregulated during somatic embryogenesis in Cichorium Biochim. Biophys. Acta. 1522 212–216 Occurrence Handle1:CAS:528:DC%2BD38Xls1ehtg%3D%3D Occurrence Handle11779636

    CAS  PubMed  Google Scholar 

  • M. Ganesan N. Jayabalan (2004) ArticleTitleEvaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L. cv. SVPR 2) Plant Cell Rep. 23 181–187 Occurrence Handle1:CAS:528:DC%2BD2cXot1ymsr4%3D Occurrence Handle15278417 Occurrence Handle10.1007/s00299-004-0822-y

    Article  CAS  PubMed  Google Scholar 

  • M.J. Gao I.A.P. Parkin D.J. Lydiate A. Hannoufa (2004) ArticleTitleAn auxin-responsive SCARECROW- like transcriptional activator interacts with histone deacetylase Plant Mol. Biol. 55 417–431 Occurrence Handle1:CAS:528:DC%2BD2MXhsFWhtQ%3D%3D Occurrence Handle15604690 Occurrence Handle10.1007/s11103-004-0892-9

    Article  CAS  PubMed  Google Scholar 

  • W.J. Gehring (1992) ArticleTitleThe homeobox in perspective Trends Biochem. Sci. 17 277–280 Occurrence Handle1:CAS:528:DyaK38XmsVKisr0%3D Occurrence Handle1357790 Occurrence Handle10.1016/0968-0004(92)90434-B

    Article  CAS  PubMed  Google Scholar 

  • R.W. Giroux K.P. Pauls (1996) ArticleTitleCharacterization of embryogenesis-related proteins in alfalfa (Medicago sativa L.) Plant Physiol. 96 585–592 Occurrence Handle1:CAS:528:DyaK28Xisleqsb4%3D

    CAS  Google Scholar 

  • R.W. Giroux K.P. Pauls (1997) ArticleTitleCharacterization of somatic embryogenesis-related cDNAs from alfalfa (Medicago sativa L.) Plant Mol. Biol. 33 393–404 Occurrence Handle1:CAS:528:DyaK2sXhvVyqsr0%3D Occurrence Handle9049261 Occurrence Handle10.1023/A:1005786826672

    Article  CAS  PubMed  Google Scholar 

  • G. Hahne J.E. Mayer H. Lorz (1988) ArticleTitleEmbryogenic and callus-specific proteins in somatic embryogenesis of the grass, DacTylis glomerata L Plant Sci. 55 267–279 Occurrence Handle1:CAS:528:DyaL1cXkslSms70%3D Occurrence Handle10.1016/0168-9452(88)90070-2

    Article  CAS  Google Scholar 

  • S. Hake E. Volbrecht M. Freeling (1989) ArticleTitleCloning Knotted, the dominant morphological mutant in maize using Ds2 as a transposon tag EMBO J. 8 15–22 Occurrence Handle1:CAS:528:DyaL1MXhs1Oitbg%3D

    CAS  Google Scholar 

  • A. Holk R. Kaldenhoff G. Richter (1996) ArticleTitleRegulation of an embryogenic carrot gene (DC 2.15) and identification of its active promoter sites Plant Mol. Biol. 31 1153–1161 Occurrence Handle1:CAS:528:DyaK28XmvFWqt7k%3D Occurrence Handle8914531 Occurrence Handle10.1007/BF00040832

    Article  CAS  PubMed  Google Scholar 

  • J.C. Kader (1996) ArticleTitleLipid-transfer proteins in plants Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 627–654 Occurrence Handle1:CAS:528:DyaK28XjtlWgt7w%3D Occurrence Handle15012303 Occurrence Handle10.1146/annurev.arplant.47.1.627

    Article  CAS  PubMed  Google Scholar 

  • N. Kamiya J.I. Itoh A. Morikami Y. Nagato M. Matsuoka (2003) ArticleTitleThe SCARECROW gene’s role in asymmetric cell divisions in rice plants Plant J. 36 45–54 Occurrence Handle1:CAS:528:DC%2BD3sXos12mtrc%3D Occurrence Handle12974810 Occurrence Handle10.1046/j.1365-313X.2003.01856.x

    Article  CAS  PubMed  Google Scholar 

  • T. Kapros L. Bogre K. Nemeth L. Bako J. GyOrgyey S.C. Wu D. Dudits (1992) ArticleTitleDifferential expression of histone H3 gene variants during cell cycle and somatic embryogenesis in alfalfa Plant Physiol. 98 621–625 Occurrence Handle1:CAS:528:DyaK38XhslSgur4%3D

    CAS  Google Scholar 

  • R. Kawahara A. Komamine H. Fukuda (1995) ArticleTitleIsolation and characterization of homeobox-containing genes of carrot Plant Mol. Biol. 27 155–164 Occurrence Handle1:STN:280:ByqC28bpvFM%3D Occurrence Handle7865785 Occurrence Handle10.1007/BF00019187

    Article  CAS  PubMed  Google Scholar 

  • J. Kehle D. Beuchle S. Treuheit B. Christen J.A. Kennison M. Bienz J. Muller (1998) ArticleTitledMi-2, a hunchback-interacting protein that functions in Polycomb repression Science 282 1897–1900 Occurrence Handle1:CAS:528:DyaK1cXotVWit7k%3D Occurrence Handle9836641 Occurrence Handle10.1126/science.282.5395.1897

    Article  CAS  PubMed  Google Scholar 

  • E. Kitamiya S. Suzuki T. Sano T. Nagata (2000) ArticleTitleIsolation of two genes that were induced upon the initiation of somatic embryogenesis on carrot hypocotyls by high concentrations of 2,4-D Plant Cell Rep. 19 551–557 Occurrence Handle1:CAS:528:DC%2BD3cXltV2lt7o%3D Occurrence Handle10.1007/s002990050772

    Article  CAS  Google Scholar 

  • Kohel, R.J. and Yu, Z.H., 1999. Cotton genomics: a review. AgBiotechNet 1: ABN 025

  • A.M. Koltunow T. Hidaka S.P. Robinson (1996) ArticleTitlePolyembryony in citrus: accumulation of seed storage proteins in seeds and in embryos cultured in vitro Plant Physiol. 110 599–609 Occurrence Handle1:CAS:528:DyaK28XhtF2ms7w%3D Occurrence Handle8742336 Occurrence Handle10.1104/pp.110.2.599

    Article  CAS  PubMed  Google Scholar 

  • R. Kumria V.G. Sunnichan D.K. Das S.K. Gupta V.S. Reddy R.K. Bhatnagar S. Leelavathi (2003) ArticleTitleHigh-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum L.) through metabolic stress Plant Cell Rep. 21 635–639 Occurrence Handle1:CAS:528:DC%2BD3sXitFakt7c%3D Occurrence Handle12789412

    CAS  PubMed  Google Scholar 

  • S.J. Kwon S.W. Hong N.S. Kim J.C. Kim (2004) ArticleTitleIsolation of callus-specific mRNAs from differentiating embryogenic somatic calli of Pimpinella brachycarpa by cDNA-AFLP Mik. Cell 17 39–44 Occurrence Handle1:CAS:528:DC%2BD2cXivF2hsrc%3D

    CAS  Google Scholar 

  • J. Lim Y. Helariutta C.D. Specht J. Jung L. Sims W.B. Bruce S. Diehn P.N. Benfey (2000) ArticleTitleMolecular analysis of the SCARECROW gene in maize reveals a common basis for radial patterning in diverse meristems Plant Cell 12 1307–1318 Occurrence Handle1:CAS:528:DC%2BD3cXmsVyjsL8%3D Occurrence Handle10948251 Occurrence Handle10.1105/tpc.12.8.1307

    Article  CAS  PubMed  Google Scholar 

  • W. Liu D.F. Hildebrand W.S. Grayburn G.C. Philips G.B. Collins (1991) ArticleTitleEffects of exogenous auxins on expression of lipoxygenases in cultured soybean embryos Plant Physiol. 97 969–976 Occurrence Handle1:CAS:528:DyaK38XhvVCluw%3D%3D

    CAS  Google Scholar 

  • C. Lurin C. Andrés S. Aubourg M. Bellaoui F. Bitton C. Bruyere M. Caboche C. Debast J. Gualberto B. Hoffmann A. Lecharny M.L. Ret M.L. Martin-Magniette H. Mireau N. Peeters J.P. Renou B. Szurek L. Taconnat I. Small (2004) ArticleTitleGenome-wide analysis of Arabidopsis pentatrico-peptide repeat proteins reveals their essential role in organelle biogenesis Plant Cell 16 2089–2103 Occurrence Handle1:CAS:528:DC%2BD2cXmvVansbc%3D Occurrence Handle15269332 Occurrence Handle10.1105/tpc.104.022236

    Article  CAS  PubMed  Google Scholar 

  • R. Mahalingam A. Gomez-Buitrago N. Eckardt N. Shah A. Guevara-Garcia P. Day R. Raina N.V. Fedoroff (2003) ArticleTitleCharacterizing the stress/defense transcriptome of Arabidopsis Genome Biol. 4 R20.1–R20.14 Occurrence Handle10.1186/gb-2003-4-3-r20

    Article  Google Scholar 

  • J. McElver D. Patton M. Rumbaugh C.M. Liu L.J. Yang D. Meinke (2000) ArticleTitleThe TITAN5 gene of Arabidopsis encodes a protein related to the ADP ribosylation factor family of GTP binding proteins Plant Cell 12 1379–1392 Occurrence Handle1:CAS:528:DC%2BD3cXmsVyjsLk%3D Occurrence Handle10948257 Occurrence Handle10.1105/tpc.12.8.1379

    Article  CAS  PubMed  Google Scholar 

  • A.H. Meijer E. Scarpella E.L. Dijk Particlevan L. Qin A.J.C. Taal S. Rueb S.E. Harrington S.R. McCouch R.A. Schilperoort J.H.C. Hoge (1997) ArticleTitleTranscriptional repression by Oshox1, a novel homeodomain leucine zipper protein from rice Plant J. 11 263–276 Occurrence Handle1:CAS:528:DyaK2sXitFCktb0%3D Occurrence Handle9076993 Occurrence Handle10.1046/j.1365-313X.1997.11020263.x

    Article  CAS  PubMed  Google Scholar 

  • R. Mishra H.Y Wang N.R. Yadav T.A. Wilkins (2003) ArticleTitleDevelopment of a highly regenerable elite acala cotton (Gossypium hirsutum cv. Maxxa): a step toward genotype-independent regeneration Plant Cell Tiss. Org. Cult. 73 21–35 Occurrence Handle1:CAS:528:DC%2BD3sXhslOkt7c%3D Occurrence Handle10.1023/A:1022666822274

    Article  CAS  Google Scholar 

  • M.C. Pedroso M.S. Pais (1995) ArticleTitleFactors controlling somatic embryogenesis Plant Cell Tiss. Org. Cult. 43 147–154 Occurrence Handle10.1007/BF00052170

    Article  Google Scholar 

  • H.J. Price R.H. Smith (1979) ArticleTitleSomatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss Planta 145 305–307 Occurrence Handle1:CAS:528:DyaE1MXitVSntLo%3D Occurrence Handle10.1007/BF00454456

    Article  CAS  Google Scholar 

  • S. Sabatini R. Heidstra M. Wildwater B. Scheres (2003) ArticleTitleSCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem Genes Dev. 17 354–358 Occurrence Handle1:CAS:528:DC%2BD3sXhtF2mtLg%3D Occurrence Handle12569126 Occurrence Handle10.1101/gad.252503

    Article  CAS  PubMed  Google Scholar 

  • Sakhanokho, H.P., Zipf, A., Sharma, G.C., Karaca, M., Saha, S. and Rajasekaran, K. 2000. Induction of highly embryogenic calli and plant regeneration in diploid and tetraploid cottons. In: National Cotton Council (Ed.), Proceedings of the Beltwide Cotton Product Research Conference. Memphis, National Cotton Council, pp. 570–575

  • S. Sato T. Toya R. Kawahara R.F. Whittier H. Fukuda A. Komamine (1995) ArticleTitleIsolation of a carrot gene expressed specifically during early-stage somatic embryogenesis Plant Mol. Biol. 28 39–46 Occurrence Handle1:CAS:528:DyaK2MXmsFemtrc%3D Occurrence Handle7787186 Occurrence Handle10.1007/BF00042036

    Article  CAS  PubMed  Google Scholar 

  • M. Schena R.W. Davis (1992) ArticleTitleHD-Zip proteins: members of an Arabidopsis homeodomain protein superfamily Proc. Natl. Acad. Sci. USA 89 3894–3898 Occurrence Handle1:CAS:528:DyaK3sXit1yntro%3D Occurrence Handle1349174

    CAS  PubMed  Google Scholar 

  • E.D.L. Schmidt F. Guzzo M.A.J. Toonen S.C. Vries Particlede (1997) ArticleTitleA leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos Development 124 2049–2062 Occurrence Handle1:CAS:528:DyaK2sXjvV2qsLc%3D Occurrence Handle9169851

    CAS  PubMed  Google Scholar 

  • K.B Singh R.C. Foley L. Onate-Sanchez (2002) ArticleTitleTranscription factors in plant defense and stress responses Curr. Opin. Plant Biol. 5 430–436 Occurrence Handle1:CAS:528:DC%2BD38XmtlGjtbw%3D Occurrence Handle12183182 Occurrence Handle10.1016/S1369-5266(02)00289-3

    Article  CAS  PubMed  Google Scholar 

  • N.R. Sinha R.E. Williams S. Hake (1993) ArticleTitleOver expression of the maize homeobox gene, KNOTTED-1, causes a switch from determinate to indeterminate cell fates Genes Dev. 7 787–795 Occurrence Handle1:CAS:528:DyaK3sXkvFWnsr0%3D Occurrence Handle7684007

    CAS  PubMed  Google Scholar 

  • L. Sossountzov L. Ruiz-Avila F. Vignols A. Jolliot V. Aroundel F. Tchang M. Grosbois F. Guerbette E. Miginiac M. Delseny (1991) ArticleTitleSpatial and temporal expression of a maize lipid transfer protein gene Plant Cell 3 923–933 Occurrence Handle1:CAS:528:DyaK38XhsVGgs70%3D Occurrence Handle1822992 Occurrence Handle10.1105/tpc.3.9.923

    Article  CAS  PubMed  Google Scholar 

  • P. Sterk H. Booij G.A. Schellekens A. Kammen Particlevan S.C. Vries Particlede (1991) ArticleTitleCell-specific expression of the carrot EP2 lipid transfer protein gene Plant Cell 3 907–921 Occurrence Handle1:CAS:528:DyaK3sXhslahtA%3D%3D Occurrence Handle1822991 Occurrence Handle10.1105/tpc.3.9.907

    Article  CAS  PubMed  Google Scholar 

  • F.C. Steward M.O. Mapes J. Smith (1958) ArticleTitleGrowth and organized development of cultured cells. I. Growth and division of freely suspended cells Am. J. Bot. 45 693–703

    Google Scholar 

  • S. Stirn A.P. Mordhorst S. Fuchs H. Lörz (1995) ArticleTitleMolecular and biochemical markers for embryogenic potential and regenerative capacity of barley (Hordeum ulgare L) cell cultures Plant Sci. 106 195–206 Occurrence Handle1:CAS:528:DyaK2MXkvVart7g%3D Occurrence Handle10.1016/0168-9452(95)04084-8

    Article  CAS  Google Scholar 

  • Z.R. Sung R. Okimoto (1981) ArticleTitleEmbryonic proteins in somatic embryos of carrot Proc. Natl. Acad. Sci. USA 78 3683–3687 Occurrence Handle1:CAS:528:DyaL3MXksFyhs78%3D

    CAS  Google Scholar 

  • K. Takahata M. Takeuchi M. Fujita J. Azuma H. Kamada F. Sato (2004) ArticleTitleIsolation of putative glycol-protein gene from early somatic embryo of carrot and its possible involvement in zsomatic embryo development Plant Cell Physiol. 45 1658–1668 Occurrence Handle1:CAS:528:DC%2BD2cXhtVGis7jK Occurrence Handle15574842 Occurrence Handle10.1093/pcp/pch188

    Article  CAS  PubMed  Google Scholar 

  • F. Thibaud-Nissen R.T. Shealy A. Khanna L.O. Vodkin (2003) ArticleTitleClustering of microarray data reveals transcript patterns associated with somatic embryogenesis in soybean Plant Physiol. 132 118–136 Occurrence Handle1:CAS:528:DC%2BD3sXktVGgs7s%3D Occurrence Handle12746518 Occurrence Handle10.1104/pp.103.019968

    Article  CAS  PubMed  Google Scholar 

  • S. Thoma U. Hecht A. Kippers J. Boletlla S. Vries Particlede C. Somerville (1994) ArticleTitleTissue-specific expression of a gene encoding a cell wall-localized lipid transfer protein from Arabidopsis Plant Physiol. 105 35–45 Occurrence Handle1:CAS:528:DyaK2cXltVSruro%3D Occurrence Handle8029357 Occurrence Handle10.1104/pp.105.1.35

    Article  CAS  PubMed  Google Scholar 

  • D.M. Tieman J.A. Ciardi M.G. Taylor H.J. Klee (2001) ArticleTitleMembers of the tomato LeEIL (EIN3-like) gene family are functionally redundant and regulate ethylene responses throughout plant development Plant J. 26 47–58 Occurrence Handle1:CAS:528:DC%2BD3MXks1Kls7Y%3D Occurrence Handle11359609 Occurrence Handle10.1046/j.1365-313x.2001.01006.x

    Article  CAS  PubMed  Google Scholar 

  • M.A.J Toonen J.A. Verhees E.D.L. Schmidt A. Kammen Particlevan S.C. Vries Particlede (1997) ArticleTitleAtLTP1 luciferase expression during carrot somatic embryogenesis Plant J. 12 1213–1221 Occurrence Handle1:CAS:528:DyaK1cXktlKhsA%3D%3D Occurrence Handle9418058 Occurrence Handle10.1046/j.1365-313X.1997.12051213.x

    Article  CAS  PubMed  Google Scholar 

  • E. Volbrecht B. Veit N. Sinha S. Hake (1991) ArticleTitleThe developmental gene Knotted-1 is a member of a maize homeobox gene family Nature 350 241–243

    Google Scholar 

  • T.A. Wilkins R. Mishra N.L. Trolinder (2004) ArticleTitleAgrobacterium-mediated transformation and regeneration of cotton J. Food, Environ. Agricult. 2 179–187

    Google Scholar 

  • T.A. Wilkins K. Rajasekaran D.M. Anderson (2000) ArticleTitleCotton biotechnology Crit. Rev. Plant Sci. 19 511–550 Occurrence Handle1:CAS:528:DC%2BD3MXhsFan

    CAS  Google Scholar 

  • J.H. Wu X.L. Zhang Y.C. Nie S.X. Jin S.G. Liang (2004) ArticleTitleFactors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cotton (Gossypium hirsutum L.) In Vitro Cell. Del. Biol. 40 371–375 Occurrence Handle1:CAS:528:DC%2BD2cXot1Oku7o%3D

    CAS  Google Scholar 

  • E.S. Wurtele H. Wang S. Durgerian B.J. Nikolau T.H. Ulrich (1993) ArticleTitleCharacterization of a gene that is expressed early in somatic embryogenesis of Daucus carota Plant Physiol. 102 303–312 Occurrence Handle1:CAS:528:DyaK3sXkvFWntrg%3D Occurrence Handle8108498 Occurrence Handle10.1104/pp.102.1.303

    Article  CAS  PubMed  Google Scholar 

  • J.W. Wysocka-Diller Y. Helariutta H. Fukaki J.E. Malamy P.N. Benfey (2000) ArticleTitleMolecular analysis of SCARECROW function reveals a radial patterning mechanism common to root and shoot Development 127 595–603 Occurrence Handle1:CAS:528:DC%2BD3cXhsVylsLg%3D Occurrence Handle10631180

    CAS  PubMed  Google Scholar 

  • X.L. Zhang J.Z. Sun J.L. Liu (1991) ArticleTitleSomatic embryogenesis and plant regeneration in upland cotton Acta Genet. Sin. 18 461–467

    Google Scholar 

  • X.L. Zhang J.M. Zhang M.J. Yiao J.Z. Sun J.L. Liu (1993) ArticleTitleStudies on somatic cell culture and its application in cotton (Gossypium hirsutum L.) improvement J. Huazhong Agri .Univ. 12 421–426

    Google Scholar 

  • J.L. Zimmerman (1993) ArticleTitleSomatic embryogenesis: a model for␣early development in higher plants Plant Cell 5 1411–1423 Occurrence Handle12271037 Occurrence Handle10.1105/tpc.5.10.1411

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, P. R. China

    Fanchang Zeng, Xianlong Zhang, Longfu Zhu, Lili Tu, Xiaoping Guo & Yichun Nie

Authors
  1. Fanchang Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xianlong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Longfu Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lili Tu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaoping Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yichun Nie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xianlong Zhang.

Additional information

*Accession numbers for the sequence data: DN762415–DN762656.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zeng, F., Zhang, X., Zhu, L. et al. Isolation and Characterization of Genes Associated to Cotton Somatic Embryogenesis by Suppression Subtractive Hybridization and Macroarray*. Plant Mol Biol 60, 167–183 (2006). https://doi.org/10.1007/s11103-005-3381-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11103-005-3381-x

Keywords

Search

Navigation