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Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco

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Abstract

Eukaryotic initiation factor eIF4E plays a pivotal role in translation initiation. As a component of the ternary eIF4F complex, eIF4E interacts with the mRNA cap structure to facilitate recruitment of the 40S ribosomal subunit onto mRNA. Plants contain two distinct cap-binding proteins, eIF4E and eIFiso4E, that assemble into different eIF4F complexes. To study the functional roles of eIF4E and eIFiso4E in tobacco, we isolated two corresponding cDNAs, NteIF4E1 and NteIFiso4E1, and used these to deplete cap-binding protein levels in planta by antisense downregulation. Antibodies raised against recombinant NteIF4E1 detected three distinct cap-binding proteins in tobacco leaf extracts; NteIF4E and two isoforms of NteIFiso4E. The three cap-binding proteins were immuno-detected in all tissues analysed and were coordinately regulated, with peak expression in anthers and pollen. Transgenic tobacco plants showing significant depletion of either NteIF4E or the two NteIFiso4E isoforms displayed normal vegetative development and were fully fertile. Interestingly, NteIFiso4E depletion resulted in a compensatory increase in NteIF4E levels, whereas the down-regulation of NteIF4E did not trigger a reciprocal increase in NteIFiso4E levels. The antisense depletion of both NteIF4E and NteIFiso4E resulted in plants with a semi-dwarf phenotype and an overall reduction in polyribosome loading, demonstrating that both eIF4E and eIFiso4E support translation initiation in planta, which suggests their potential role in the regulation of plant growth.

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Authors and Affiliations

  1. Department of Biology, University of Leicester, LE1 7RH, Leicester, United Kingdom

    Jonathan P. Combe & David Twell

  2. Department of Biochemistry and Molecular Biology, Oklahoma State University, 246 NRC, Stillwater, OK, 74078, USA

    Marie E. Petracek

  3. Bayer BioScience N.V., Technologie park 38, B-9052, Gent, Belgium

    Gerben van Eldik & Frank Meulewaeter

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  1. Jonathan P. Combe
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  2. Marie E. Petracek
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  3. Gerben van Eldik
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  4. Frank Meulewaeter
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  5. David Twell
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Correspondence to David Twell.

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Combe, J.P., Petracek, M.E., van Eldik, G. et al. Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco. Plant Mol Biol 57, 749–760 (2005). https://doi.org/10.1007/s11103-005-3098-x

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