Photosynthetica 2018, 56(1):200-209 | DOI: 10.1007/s11099-018-0788-6

PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803

S. Biswas1, J. J. Eaton-Rye1,*
1 Department of Biochemistry, University of Otago, Dunedin, New Zealand

The PsbM (3.9 kDa) and PsbY (4.2 kDa) proteins are membrane-spanning, single-helix, subunits associated with the chlorophyll-binding CP47 pre-complex of photosystem II (PSII). Removal of PsbM resulted in accumulation of PSII pre-assembly complexes and impaired electron transfer between the primary (QA) and secondary (QB) plastoquinone electron acceptors of PSII indicating that the QB-binding site and bicarbonate binding to the non-heme iron were altered in this strain. Removal of PsbY alone had only a minor impact on PSII activity but deleting PsbY in the ΔPsbM background led to additional modification of the acceptor side resulting in ΔPsbM:ΔPsbY cells being susceptible to photodamage and this required protein synthesis for recovery. Addition of bicarbonate was able to compensate for the light-induced damage in ΔPsbM:ΔPsbY cells potentially re-occupying the modified bicarbonate-binding site in the ΔPsbM:ΔPsbY strain and complementation of ΔPsbM:ΔPsbY cells with the psbY gene restored the ΔPsbM phenotype.

Additional key words: assembly; bicarbonate; chlorophyll fluorescence; cytochrome b559; photoinhibition; repair

Received: October 3, 2017; Accepted: December 19, 2017; Published: March 1, 2018  Show citation

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Biswas, S., & Eaton-Rye, J.J. (2018). PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803. Photosynthetica, 56(SPECIAL ISSUE), 200-209. doi: 10.1007/s11099-018-0788-6
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