Photosynthetica 2016, 54(3):422-429 | DOI: 10.1007/s11099-016-0185-y

Chlorophyll a fluorescence analysis of high-yield rice (Oryza sativa L.) LYPJ during leaf senescence

Y. W. Wang1, C. Xu1, C. F. Lv1, M. Wu1,2, X. J. Cai1, Z. T. Liu1, X. M. Song1, G. X. Chen1,*, C. G. Lv3
1 Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, China
2 Zijin College, Nanjing University of Science and Technology, Nanjing, China
3 Institute of Food and Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China

Photosystem II (PSII) photochemistry was examined by chlorophyll (Chl) a fluorescence analysis in high-yield rice LYPJ flag leaves during senescence. Parameters deduced from the JIP-test showed that inhibition of the donor side of PSII was greater than that of the acceptor side in hybrid rice LYPJ. The natural senescence process was accompanied by the increased inactivation of oxygen-evolving complex (OEC) and a lower total number of active reaction centers per absorption. It indicated that the inhibition of electron transport caused by natural senescence might be caused partly by uncoupling of the OEC and/or inactivation of PSII reaction centers. Chl fluorescence parameters analyzed in this study suggested that energy dissipation was enhanced in order to protect senescent leaves from photodamage. Nevertheless, considerably reduced PSI electron transport activity was observed at the later senescence. Thus, natural senescence inhibited OEC-PSII electron transport, but also significantly limited the PSII-PSI electron flow.

Additional key words: JIP-test; natural senescence; PSII efficiency; thylakoid membrane

Received: April 14, 2015; Accepted: November 5, 2015; Published: September 1, 2016  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Wang, Y.W., Xu, C., Lv, C.F., Wu, M., Cai, X.J., Liu, Z.T., ... Lv, C.G. (2016). Chlorophyll a fluorescence analysis of high-yield rice (Oryza sativa L.) LYPJ during leaf senescence. Photosynthetica, 54(3), 422-429. doi: 10.1007/s11099-016-0185-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alberta K.R., Mikkelsen T.N., Ro-Poulsen H.: Ambient UV-B radiation decreases photosynthesis in high arctic Vaccinium uliginosum.-Physiol. Plantarum 133: 199-210, 2008. Go to original source...
    2. Boureima S., Oukarroum A., Diouf M. et al.: Screening for drought tolerance in mutant germplasm of sesame (Sesamum indicum) probing by chlorophyll a fluorescence.-Environ. Exp. Bot. 81: 37-43, 2012. Go to original source...
    3. Breątic M., ®ivcák M., Oląovská K. et al.: Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves.-Plant Physiol. Biochem. 81: 74-83, 2014. Go to original source...
    4. Chen C., Chen H., Lin Y.S. et al.: A two-locus interaction causes interspecific hybrid weakness in rice.-Nat. Commun. 5: 3357-3367, 2014. Go to original source...
    5. Chhotaray D., Chandrakala Y., Mishra C.S.K. et al.: Farm practices influence the photosynthetic performance and plant efficiency of Oryza sativa L.-Acta Physiol. Plant. 36: 1501-1511, 2014. Go to original source...
    6. Ceppi M.G., Oukarroum A., Çiçek N. et al.: The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves: a study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress.-Physiol. Plantarum 144: 277-288, 2012. Go to original source...
    7. D'Amici G.M., Timperio A.M., Zolla L.: Coupling of native liquid phase electrofocusing and blue native polyacrylamide gel electrophoresis: a potent tool for native membrane multiprotein complex separation.-J. Proteome Res. 7: 1326-1240, 2008. Go to original source...
    8. Feng B., Liu P., Li G. et al.: Effect of heat stress on the photosynthetic characteristics in flag leaves at the grain-Filling stage of different heat-resistant winter wheat varieties.-J. Agro. Crop. Sci. 200: 143-155, 2014. Go to original source...
    9. Holland V., Koller S., Brüggemann W.: Insight into the photosynthetic apparatus in evergreen and deciduous European oaks during autumn senescence using OJIP fluorescence transient analysis.-Plant Biol. 16: 801-808, 2014. Go to original source...
    10. Hsu B.D.: On the possibility of using a chlorophyll fluorescence parameter as an indirect indicator for the growth of Phalaenopsis seedlings.-Plant Sci. 172: 604-608, 2007. Go to original source...
    11. Hussain M.I., Reigosa M.J.: A chlorophyll fluorescence analysis of photosynthetic efficiency, quantum yield and photon energy dissipation in PSII antennae of Lactuca sativa L. leaves exposed to cinnamic acid.-Plant Physiol. Biochem. 49: 1290-1298, 2011. Go to original source...
    12. Jiao D., Ji B., Li X.: Characteristics of chlorophyll fluorescence and membrane-lipid peroxidation during senescence of flag leaf in different cultivars of rice.-Photosynthetica 41: 33-41, 2003. Go to original source...
    13. Kalachanis D., Manetas Y.: Analysis of fast chlorophyll fluorescence rise (O-K-J-I-P) curves in green fruits indicates electron flow limitations at the donor side of PSII and the acceptor sides of both photosystems.-Physiol. Plantarum 139: 313-323, 2010. Go to original source...
    14. Kalaji H.M., Oukarroum A., Alexandrov V. et al.: Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements.-Plant Physiol. Biochem. 81: 16-25, 2014. Go to original source...
    15. Kreslavski V.D., Lankin A.V., Vasilyeva G.K. et al.: Effects of polyaromatic hydrocarbons on photosystem II activity in pea leaves.-Plant Physiol. Biochem. 81: 135-142, 2014. Go to original source...
    16. Kumar K.S., Dahms H.U., Lee J.S. et al.: Algal photosynthetic responses to toxic metals and herbicides assessed by chlorophyll a fluorescence.-Ecotoxicol. Environ. Safe. 104: 51-71, 2014. Go to original source...
    17. Kügler M., Jänsch L., Kruft V. et al.: Analysis of the chloroplast protein complexes by blue-native polyacrylamide gel electrophoresis (BN-PAGE).-Photosynth. Res. 53: 35-44, 1997. Go to original source...
    18. Lazár D.: The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light.-Funct. Plant Biol. 33: 9-30, 2006. Go to original source...
    19. Lazár D., Nauą J.: Statistical properties of chlorophyll fluorescence induction parameters.-Photosynthetica 35: 121-127, 1998. Go to original source...
    20. Li X.M., Chen M.J., Li J. et al.: Effect of endophyte infection on chlorophyll a fluorescence in salinity stressed rice.-Biol. Plantarum 58: 589-594, 2014. Go to original source...
    21. Mohapatra P.K., Khillar R., Hansdah B. et al.: Photosynthetic and fluorescence responses of Solanum melangena L. to field application of dimethoate.-Ecotoxicol. Environ. Safe. 73: 78-83, 2010. Go to original source...
    22. Osório J., Osório M.L., Correia P.J. et al.: Chlorophyll fluorescence imaging as a tool to understand the impact of iron deficiency and resupply on photosynthetic performance of strawberry plants.-Sci. Hortic.-Amsterdam 165: 148-155, 2014. Go to original source...
    23. Panda D., Sarkar R.K.: Natural leaf senescence: probed by chlorophyll fluorescence, CO2 photosynthetic rate and antioxidant enzyme activities during grain filling in different rice cultivars.-Physiol. Mol. Biol. Plants 19: 43-51, 2013. Go to original source...
    24. Pollastrini M., Holland V., Brüggemann W. et al.: Interactions and competition processes among tree species in young experimental mixed forests, assessed with chlorophyll fluorescence and leaf morphology.-Plant Biol. 16: 323-331, 2014. Go to original source...
    25. Qiu Z., Wang L., Zhou Q.: Effects of bisphenol A on growth, photosynthesis and chlorophyll fluorescence in above-ground organs of soybean seedlings.-Chemosphere 90: 1274-1280, 2013. Go to original source...
    26. Schansker G., Tóth S.Z., Strasser R.J.: Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP.-Biochim. Biophys. Acta 1706: 250-261, 2005. Go to original source...
    27. Sharma D.K., Fernández J.O., Rosenqvist E. et al.: Genotypic response of detached leaves versus intact plants for chlorophyll fluorescence parameters under high temperature stress in wheat.-J. Plant Physiol. 171: 576-586, 2014. Go to original source...
    28. Shen W.J., Chen G.X., Xu J.G. et al.: Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane.-Photosynthetica 53: 436-446, 2015. Go to original source...
    29. Stefanov D., Petkova V., Denev I.D.: Screening for heat tolerance in common bean (Phaseolus vulgaris L.) lines and cultivars using JIP-test.-Sci. Hortic.-Amsterdam 128: 1-6, 2011. Go to original source...
    30. Stirbet A., Govindjee: On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and Photosystem II: Basics and applications of the OJIP fluorescence transient.-J. Photochem. Photobiol. B 104: 236-257, 2011. Go to original source...
    31. Strasser R.J., Srivastava A., Govindjee: Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria.-Photochem. Photobiol. 61: 32-42, 1995. Go to original source...
    32. Strasser B.J., Strasser R.J.: Measuring fast fluorescence transients to address environmental questions: The JIP-test.-In: Mathis P (ed.): Photosynthesis: from Light to Biosphere. Pp. 977-980. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    33. Tang Y.L., Wen X.G., Lu C.M.: Differential changes in degradation of chlorophyll-protein complexes of photosystem I and photosystem II during flag leaf senescence of rice.-Plant Physiol. Bioch. 43: 193-201, 2005. Go to original source...
    34. Timperio A.M., D'Amici G.M., Barta C. et al.: Proteomics, pigment composition, and organization of thylakoid membranes in iron-deficient spinach leaves.-J. Exp. Bot. 58: 3695-3710, 2007. Go to original source...
    35. Tsimilli-Michael M., Strasser R.J.: In vivo assessment of plants' vitality: applications in detecting and evaluating the impact of Mycorrhization on host plants.-In: Varma A. (ed.): Mycorrhiza: State of the Art, Genetics and Molecular Biology, Ecofunction, Biotechnology, Eco-Physiology, Structure and Systematics (3rd edition). Pp. 679-703. Springer, Dordrecht 2008. Go to original source...
    36. Wang G., Hao Z., Anken R.H. et al.: Effects of UV-B radiation on photosynthesis activity of Wolffia arrhiza as probed by chlorophyll fluorescence transients.-Adv. Space Res. 45: 839-845, 2010a. Go to original source...
    37. Wang L.J., Fan L., Loescher W. et al.: Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves.-BMC Plant Biol. 10: 34-43, 2010b. Go to original source...
    38. Wang Y.W., Zhang J.J., Yu J. et al.: Photosynthetic changes of flag leaves during senescence stage in super high-yield hybrid rice LYPJ grown in field condition.-Plant Physiol. Bioch. 82: 194-201, 2014. Go to original source...
    39. Wu H.B., Wang B., Chen Y. et al.: Characterization and fine mapping of the rice premature senescence mutant ospse1.-Theor. Appl. Genet. 126: 1897-1907, 2013. Go to original source...
    40. Wu M., Wang P.Y., Sun L.G. et al.: Alleviation of cadmium toxicity by cerium in rice seedlings is related to improved photosynthesis, elevated antioxidant enzymes and decreased oxidative stress.-Plant Growth Regul. 74: 251-260, 2014. Go to original source...
    41. Xia J., Li Y., Zou D.: Effects of salinity stress on PSII in Ulva lactuca as probed by chlorophyll fluorescence measurements.-Aquat. Bot. 80: 129-137, 2004. Go to original source...
    42. Yu G.H., Li W., Yuan Z.Y. et al.: The effects of enhanced UV-B radiation on photosynthetic and biochemical activities in superhigh-yield hybrid rice Liangyoupeijiu at the reproductive stage.-Photosynthetica 51: 33-44, 2013. Go to original source...
    43. Zhang C.J., Chu H.J., Chen G.X. et al.: Photosynthetic and biochemical activities in flag leaves of a newly developed super high-yield hybrid rice (Oryza sativa) and its parents during the reproductive stage.-J. Plant Res. 120: 209-217, 2007. Go to original source...

    Return to the content