Photosynthetica 2008, 46(4):581 | DOI: 10.1007/s11099-008-0098-5

Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling

W. H. Hu1,3, X. S. Song1, K. Shi1, X. J. Xia1, Y. H. Zhou1, J. Q. Yu1,2,*
1 Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou, P.R. China
2 Key Laboratory of Horticultural Plants Growth, Development and Biotechnology, Agricultural Ministry of China, Hangzhou, P.R. China
3 Department of Life Science, Jinggangshan University, Ji'an, P.R. China

In order to clarify the relationship between chill-induced disturbance in photosynthetic, respiratory electron transport and the metabolism of reactive oxygen species (ROS), leaf gas exchange, chlorophyll fluorescence quenching, respiration, and activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) were investigated in chloroplasts and mitochondria of cucumber (Cucumis sativus) leaves subjected to a chill (8 °C) for 4 d. Chilling decreased net photosynthetic rate (P N) and quantum efficiency of photosystem 2 (ΦPS2), but increased the ratio of ΦPS2 to the quantum efficiency of CO2 fixation (ΦCO2) and non-photochemical quenching (NPQ) in cucumber leaves. While chilling inhibited the activity of cytochrome respiration pathway, it induced an increase of alternative respiration pathway activity and the reduction level of Q-pool. Chilling also significantly increased O2 * production rate, H2O2 content, and SOD and APX activities in chloroplasts and mitochondria. There was a more significant increase in SOD and APX activities in chloroplasts than in mitochondria with the increase of membrane-bound Fe-SOD and tAPX in chloroplasts being more significant than other isoenzymes. Taken together, chilling inhibited P N and cytochrome respiratory pathway but enhanced the photosynthetic electron flux to O2 and over-reduction of respiratory electron transport chain, resulting in ROS accumulation in cucumber leaves. Meanwhile, chilling resulted in an enhancement of the protective mechanisms such as thermal dissipation, alternative respiratory pathway, and ROS-scavenging mechanisms (SODs and APXs) in chloroplasts and mitochondria.

Additional key words: ascorbate peroxidase; Cucumis sativus; net photosynthetic rate; photosynthetic electron transport; quantum efficiency; reactive oxidative metabolism; respiratory electron transport; respiration pathways; superoxide dismutase

Received: February 6, 2008; Accepted: July 16, 2008; Published: December 1, 2008  Show citation

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Hu, W.H., Song, X.S., Shi, K., Xia, X.J., Zhou, Y.H., & Yu, J.Q. (2008). Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling. Photosynthetica, 46(4), 581. doi: 10.1007/s11099-008-0098-5
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