ABSTRACT
Purpose
To produce and examine the aerosol performance of protein nano-matrix particles with different surface roughness.
Methods
Aqueous lysozyme solutions were poured into isopropanol during high-shear mixing to produce nanoparticles by precipitation. The size of the nanoparticles was varied by adjusting the precipitation conditions. The resultant suspensions were spray-dried to obtain micron-sized aggregates (nano-matrices). Smooth particles were made by spray-drying a lysozyme solution. The aggregate size distribution, surface roughness, and cohesion were evaluated. The aerosol performance was assessed by dispersing 10 mg of powder from a Rotahaler® at 60 L/min or an Aerolizer® at 100 L/min into a Next Generation Impactor, followed by chemical assay (n = 3).
Results
The median volume diameter and span of the nano-matrix particles were 1.0–1.2 μm and 1.5–1.6, respectively, which were comparable to those of the smooth particles. Surface roughness increased with the size of the primary nanoparticles. The nano-matrix particles were significantly less cohesive than the smooth particles. The fine particle fraction increased linearly with increasing surface roughness and decreasing cohesion.
Conclusions
Nano-matrix particles with controlled surface architecture were successfully produced by spray-drying nanosuspensions. Aerosol performance was enhanced with increasing surface roughness due to the reduction in cohesion forces.
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ACKNOWLEDGMENTS
The work was financially supported by the Australian Research Council (Discovery Project 0985367).
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Kwok, P.C.L., Tunsirikongkon, A., Glover, W. et al. Formation of Protein Nano-Matrix Particles with Controlled Surface Architecture for Respiratory Drug Delivery. Pharm Res 28, 788–796 (2011). https://doi.org/10.1007/s11095-010-0332-2
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DOI: https://doi.org/10.1007/s11095-010-0332-2