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Towards More Realistic In Vitro Release Measurement Techniques for Biodegradable Microparticles

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Abstract

Purpose

To better understand the importance of the environmental conditions for drug release from biodegradable microparticles allowing for the development of more appropriate in vitro release measurement techniques.

Methods

Propranolol HCl diffusion in various agarose gels was characterized by NMR and UV analysis. Fick’s law was used to theoretically predict the mass transport kinetics. Drug release from PLGA-based microparticles in such agarose gels was compared to that measured in agitated bulk fluids (“standard” method).

Results

NMR analysis revealed that the drug diffusivity was almost independent of the hydrogel concentration, despite of the significant differences in the systems’ mechanical properties. This is due to the small size of the drug molecules/ions with respect to the hydrogel mesh size. Interestingly, the theoretically predicted drug concentration-distance-profiles could be confirmed by independent experiments. Most important from a practical point of view, significant differences in the release rates from the same batch of PLGA-based microparticles into a well agitated bulk fluid versus a semi-solid agarose gel were observed.

Conclusion

Great care must be taken when defining the in vitro conditions for drug release measurements from biodegradable microparticles. The obtained new insight can help facilitating the development of more appropriate in vitro release testing procedures.

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Acknowledgements

The authors are grateful for the support of this work by the French Association for Cancer Research “ARC” (“Association pour la Recherche sur le Cancer”: postdoctoral fellowship for Dr. Florence Siepmann and doctoral fellowship for Mrs. Diana Klose). The NMR facilities were funded by the “Nord-Pas de Calais” Regional Council, the French Ministry and European Regional Development Fonds (FEDER).

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Authors and Affiliations

  1. College of Pharmacy, JE 2491, University of Lille, 3 rue du Professeur Laguesse, 59006, Lille, France

    D. Klose, F. Siepmann & J. Siepmann

  2. College of Pharmacy, Freie Universitaet Berlin, Kelchstr. 31, 12169, Berlin, Germany

    D. Klose

  3. College of Pharmacy, CNRS UMR 8009, University of Lille, 3 rue du Professeur Laguesse, 59006, Lille, France

    N. Azaroual & G. Vermeersch

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  1. D. Klose
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Correspondence to J. Siepmann.

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Klose, D., Azaroual, N., Siepmann, F. et al. Towards More Realistic In Vitro Release Measurement Techniques for Biodegradable Microparticles. Pharm Res 26, 691–699 (2009). https://doi.org/10.1007/s11095-008-9747-4

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  • DOI: https://doi.org/10.1007/s11095-008-9747-4

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