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Development and Validation of an HPLC Method with Mass Spectrometric Detection for Quantitative Determination of Chlormadinone Acetate and Ethinyl Estradiol in Human Blood Plasma

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An HPLC method with mass-spectrometric detection was developed for simultaneous quantitative determination of chlormadinone acetate and ethinyl estradiol in human blood plasma. The sample preparation method included liquid—liquid extraction using hexane—Et2O. The method was validated for selectivity, linearity, accuracy, precision, cross-transfer, and stability. The developed method enabled simultaneous determination of the chlormadinone acetate and ethinyl estradiol blood concentrations in volunteers and could be used for pharmacokinetics studies.

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Authors and Affiliations

  1. Sechenov First Moscow State Medical University, Moscow, 128018, Russia

    G. N. Gil’deeva & A. V. Belostotskii

  2. MDA Ltd., Moscow, Russia

    D. S. Sapozhnikova

Authors
  1. G. N. Gil’deeva
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  2. A. V. Belostotskii
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  3. D. S. Sapozhnikova
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Corresponding author

Correspondence to D. S. Sapozhnikova.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 51, No. 2, pp. 46 – 56, February, 2017.

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Gil’deeva, G.N., Belostotskii, A.V. & Sapozhnikova, D.S. Development and Validation of an HPLC Method with Mass Spectrometric Detection for Quantitative Determination of Chlormadinone Acetate and Ethinyl Estradiol in Human Blood Plasma. Pharm Chem J 51, 142–152 (2017). https://doi.org/10.1007/s11094-017-1573-8

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  • DOI: https://doi.org/10.1007/s11094-017-1573-8

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